Literature summary extracted from
Xu, M.; Hillwig, M.L.; Prisic, S.; Coates, R.M.; Peters, R.J.
Functional identification of rice syn-copalyl diphosphate synthase and its role in initiating biosynthesis of diterpenoid phytoalexin/allelopathic natural products (2004), Plant J., 39, 309-318.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
5.5.1.14 |
expression of the full-length preprotein including the transit peptid in Escherichia coli, protein alone and as a C-terminal fusion to glutathione-S-transferase. Additionally, a partial cDNA sequence resembling the mature native protein was expressed alone as a potential pseudomature protein |
Oryza sativa |
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
5.5.1.14 |
plastid |
deduced from the existence of a transit peptide sequence |
Oryza sativa |
9536 |
- |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
5.5.1.14 |
geranylgeranyl diphosphate |
Oryza sativa |
- |
syn-copalyl diphosphate |
precursor of the phytoalexins oryzalexin S, momilactone A, and momilactone B |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
5.5.1.14 |
Oryza sativa |
Q6E7D7 |
indica cultivar-group |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
5.5.1.14 |
hydroxyapatite chromatography, only partially purified |
Oryza sativa |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
5.5.1.14 |
geranylgeranyl diphosphate = 9alpha-copalyl diphosphate |
in contrast to EC 5.5.1.12 and EC 5.5.1.13, this enzyme produces copalyl diphosphate having the syn conformation |
Oryza sativa |
|
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
5.5.1.14 |
leaf |
- |
Oryza sativa |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
5.5.1.14 |
geranylgeranyl diphosphate |
- |
Oryza sativa |
syn-copalyl diphosphate |
precursor of the phytoalexins oryzalexin S, momilactone A, and momilactone B |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
5.5.1.14 |
OsCPSsyn |
- |
Oryza sativa |
5.5.1.14 |
syn-copalyl diphosphate synthase |
- |
Oryza sativa |