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Literature summary extracted from

  • Inui, T.; Tamura, K.; Fujii, N.; Morishige, T.; Sato, F.
    Overexpression of Coptis japonica norcoclaurine 6-O-methyltransferase overcomes the rate-limiting step in Benzylisoquinoline alkaloid biosynthesis in cultured Eschscholzia californica (2007), Plant Cell Physiol., 48, 252-262.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.128 synthesis production of the economically important analgesic morphine and the antimicrobial agent berberine Eschscholzia californica
2.1.1.128 synthesis production of the economically important analgesic morphine and the antimicrobial agent berberine Coptis japonica

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.116 full-length cDNA of Coptis japonica 4'OMT is cloned into the binary vector pBITXEl2 for introduction into Agrobacterium tumefaciens, for infection Eschscholzia californica seedlings are co-cultured with Agrobacterium tumefaciens Coptis japonica
2.1.1.116 into the pET-21d vector for expression in Escherichia coli BL21DE3 cells Coptis japonica
2.1.1.116 into the pET-41a+ vector for expression in Escherichia coli BL21DE3 cells Eschscholzia californica
2.1.1.128 expressed in Escherichia coli Eschscholzia californica
2.1.1.128 expressed in Eschscholzia californica Coptis japonica
2.1.1.128 full-length cDNA of Coptis japonica 6OMT is cloned into the binary vector pBITXEl2 for introduction into Agrobacterium tumefaciens, for infection Eschscholzia californica seedlings are co-cultured with Agrobacterium tumefaciens Coptis japonica
2.1.1.128 into the pET-21d vector for expression in Escherichia coli BL21DE3 cells Coptis japonica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.128 40000
-
 x * 40000, SDS-PAGE, recombinant Ec4’OMT Eschscholzia californica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.116 S-adenosyl-L-homocysteine + (S)-reticuline Eschscholzia californica
-
 S-adenosyl-L-methionine + 3'-hydroxy-N-methyl-(S)-coclaurine
-
 ?
2.1.1.116 S-adenosyl-L-homocysteine + (S)-reticuline Coptis japonica
-
 S-adenosyl-L-methionine + 3'-hydroxy-N-methyl-(S)-coclaurine
-
 ?
2.1.1.116 S-Adenosyl-L-methionine + 3'-hydroxy-N-methyl-(S)-coclaurine Coptis japonica
-
 S-Adenosyl-L-homocysteine + (S)-reticuline
-
 ?
2.1.1.128 S-adenosyl-L-homocysteine + (S)-reticuline Coptis japonica
-
 S-adenosyl-L-methionine + 3'-hydroxy-N-methyl-(S)-coclaurine
-
 ?
2.1.1.128 S-adenosyl-L-methionine + (S)-norcoclaurine Coptis japonica
-
 S-adenosyl-L-homocysteine + (S)-coclaurine
-
 ?
2.1.1.128 S-adenosyl-L-methionine + (S)-norcoclaurine Coptis japonica putative rate-limiting step enzymes in benzylisoquinoline alkaloid biosynthesis S-adenosyl-L-homocysteine + (S)-coclaurine
-
 ?
2.1.1.128 S-adenosyl-L-methionine + (S)-norlaudanosoline Eschscholzia californica putative rate-limiting step enzymes in benzylisoquinoline alkaloid biosynthesis S-adenosyl-L-homocysteine + 6-O-methylnorlaudanosoline
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.116 Coptis japonica Q9LEL5
-
-
2.1.1.116 Eschscholzia californica
-
-
-
2.1.1.128 Coptis japonica Q9LEL6
-
-
2.1.1.128 Eschscholzia californica
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.116 seedling
-
 Coptis japonica
-
2.1.1.128 seedling
-
 Coptis japonica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.116 S-adenosyl-L-homocysteine + (S)-reticuline
-
 Eschscholzia californica S-adenosyl-L-methionine + 3'-hydroxy-N-methyl-(S)-coclaurine
-
 ?
2.1.1.116 S-adenosyl-L-homocysteine + (S)-reticuline
-
 Coptis japonica S-adenosyl-L-methionine + 3'-hydroxy-N-methyl-(S)-coclaurine
-
 ?
2.1.1.116 S-Adenosyl-L-methionine + 3'-hydroxy-N-methyl-(S)-coclaurine
-
 Coptis japonica S-Adenosyl-L-homocysteine + (S)-reticuline
-
 ?
2.1.1.128 S-adenosyl-L-homocysteine + (S)-reticuline
-
 Coptis japonica S-adenosyl-L-methionine + 3'-hydroxy-N-methyl-(S)-coclaurine
-
 ?
2.1.1.128 S-adenosyl-L-methionine + (S)-norcoclaurine
-
 Eschscholzia californica S-adenosyl-L-homocysteine + (S)-coclaurine
-
 ?
2.1.1.128 S-adenosyl-L-methionine + (S)-norcoclaurine
-
 Coptis japonica S-adenosyl-L-homocysteine + (S)-coclaurine
-
 ?
2.1.1.128 S-adenosyl-L-methionine + (S)-norcoclaurine putative rate-limiting step enzymes in benzylisoquinoline alkaloid biosynthesis Coptis japonica S-adenosyl-L-homocysteine + (S)-coclaurine
-
 ?
2.1.1.128 S-adenosyl-L-methionine + (S)-norlaudanosoline putative rate-limiting step enzymes in benzylisoquinoline alkaloid biosynthesis Eschscholzia californica S-adenosyl-L-homocysteine + 6-O-methylnorlaudanosoline
-
 ?
2.1.1.128 S-adenosyl-L-methionine + laudanosoline low activity Eschscholzia californica ?
-
 ?
2.1.1.128 S-adenosyl-L-methionine + laudanosoline low activity Coptis japonica ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.128 ? x * 40000, SDS-PAGE, recombinant Ec4’OMT Eschscholzia californica

Synonyms

EC Number Synonyms Comment Organism
2.1.1.116 3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase
-
 Eschscholzia californica
2.1.1.116 3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase
-
 Coptis japonica
2.1.1.116 4'OMT
-
 Eschscholzia californica
2.1.1.116 4'OMT
-
 Coptis japonica
2.1.1.128 3’-hydroxy-N-methylcoclaurine 4’-O-methyltransferase Ec4’OMT shows clear 6-O-methylation activity for norcoclaurine and produced coclaurine Eschscholzia californica
2.1.1.128 6OMT
-
 Coptis japonica
2.1.1.128 Ec4’OMT
-
 Eschscholzia californica
2.1.1.128 Norcoclaurine 6-O-methyltransferase
-
 Eschscholzia californica
2.1.1.128 Norcoclaurine 6-O-methyltransferase
-
 Coptis japonica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.116 30
-
 OMT enzyme assay Eschscholzia californica
2.1.1.116 30
-
 OMT enzyme assay Coptis japonica
2.1.1.128 30
-
 OMT enzyme assay Coptis japonica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.116 7.5
-
 OMT enzyme assay Eschscholzia californica
2.1.1.116 7.5
-
 OMT enzyme assay Coptis japonica
2.1.1.128 7.5
-
 OMT enzyme assay Coptis japonica