BRENDA - Enzyme Database

A heteromeric plastidic pyruvate kinase complex involved in seed oil biosynthesis in Arabidopsis

Andre, C.; Froehlich, J.E.; Moll, M.R.; Benning, C.; Plant Cell 19, 2006-2022 (2007)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.40
6-phosphogluconate
192% of activity at 0.05 mM
Arabidopsis thaliana
2.7.1.40
additional information
not activated by 6-phosphogluconate
Arabidopsis thaliana
Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.40
expressed in Escherichia coli
Arabidopsis thaliana
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.7.1.40
K314L
no increased activity at pH 7.0
Arabidopsis thaliana
2.7.1.40
K325L
no increased activity at pH 7.0
Arabidopsis thaliana
2.7.1.40
K344L
no increased activity at pH 7.0
Arabidopsis thaliana
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.40
AMP
1 mM, 77% of activity remaining
Arabidopsis thaliana
2.7.1.40
ATP
1 mM, 77% of activity remaining; 1 mM, 87% of activity remaining
Arabidopsis thaliana
2.7.1.40
glyoxylate
5 mM, 79% of activity remaining; 5 mM, 83% of activity remaining
Arabidopsis thaliana
2.7.1.40
iso-citrate
10 mM, 70% of activity remaining; 10 mM, 75% of activity remaining
Arabidopsis thaliana
2.7.1.40
L-glutamate
5 mM, 30% of activity remaining, IC50: 2.1 mM, IC50: 6.2 mM; 5 mM, 59% of activity remaining, IC50: 6.2 mM
Arabidopsis thaliana
2.7.1.40
additional information
not inhibited by 6-phosphogluconate, dithiothreitol or sodium tetrathionate; not inhibited by AMP, dithiothreitol or sodium tetrathionate
Arabidopsis thaliana
2.7.1.40
oxalate
0.2 mM, 50% of activity remaining; 0.2 mM, 71% of activity remaining, IC50: 0.41 mM
Arabidopsis thaliana
2.7.1.40
oxaloacetate
2 mM, 82% of activity remaining; 2 mM, 88% of activity remaining
Arabidopsis thaliana
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.40
plastid
-
Arabidopsis thaliana
9536
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.40
K+
strict requirement
Arabidopsis thaliana
2.7.1.40
Mg2+
strict requirement
Arabidopsis thaliana
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.40
57000
-
4 * 60000 + 4 * 57000, gel filtration
Arabidopsis thaliana
2.7.1.40
60000
-
4 * 60000 + 4 * 57000, gel filtration
Arabidopsis thaliana
2.7.1.40
463000
-
gel filtration
Arabidopsis thaliana
2.7.1.40
476000
-
gel filtration
Arabidopsis thaliana
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.40
Arabidopsis thaliana
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.40
Ni-NTA resin chromatography
Arabidopsis thaliana
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.40
seed
-
Arabidopsis thaliana
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.40
ADP + phosphoenolpyruvate
-
676434
Arabidopsis thaliana
ATP + pyruvate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.40
octamer
4 * 60000 + 4 * 57000, gel filtration
Arabidopsis thaliana
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.1.40
50
55
completely inactivated after 3 min of incubation at 50 to 55°C
Arabidopsis thaliana
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.40
8
-
-
Arabidopsis thaliana
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.40
ADP
pyruvate kinase activity is not observed in the absence of ADP
Arabidopsis thaliana
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
2.7.1.40
0.41
-
0.2 mM, 71% of activity remaining, IC50: 0.41 mM
Arabidopsis thaliana
oxalate
2.7.1.40
6.2
-
5 mM, 30% of activity remaining, IC50: 2.1 mM, IC50: 6.2 mM; 5 mM, 59% of activity remaining, IC50: 6.2 mM
Arabidopsis thaliana
L-glutamate
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.40
6-phosphogluconate
192% of activity at 0.05 mM
Arabidopsis thaliana
2.7.1.40
additional information
not activated by 6-phosphogluconate
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.40
expressed in Escherichia coli
Arabidopsis thaliana
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.40
ADP
pyruvate kinase activity is not observed in the absence of ADP
Arabidopsis thaliana
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.7.1.40
K314L
no increased activity at pH 7.0
Arabidopsis thaliana
2.7.1.40
K325L
no increased activity at pH 7.0
Arabidopsis thaliana
2.7.1.40
K344L
no increased activity at pH 7.0
Arabidopsis thaliana
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
2.7.1.40
0.41
-
0.2 mM, 71% of activity remaining, IC50: 0.41 mM
Arabidopsis thaliana
oxalate
2.7.1.40
6.2
-
5 mM, 30% of activity remaining, IC50: 2.1 mM, IC50: 6.2 mM; 5 mM, 59% of activity remaining, IC50: 6.2 mM
Arabidopsis thaliana
L-glutamate
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.40
AMP
1 mM, 77% of activity remaining
Arabidopsis thaliana
2.7.1.40
ATP
1 mM, 77% of activity remaining; 1 mM, 87% of activity remaining
Arabidopsis thaliana
2.7.1.40
glyoxylate
5 mM, 79% of activity remaining; 5 mM, 83% of activity remaining
Arabidopsis thaliana
2.7.1.40
iso-citrate
10 mM, 70% of activity remaining; 10 mM, 75% of activity remaining
Arabidopsis thaliana
2.7.1.40
L-glutamate
5 mM, 30% of activity remaining, IC50: 2.1 mM, IC50: 6.2 mM; 5 mM, 59% of activity remaining, IC50: 6.2 mM
Arabidopsis thaliana
2.7.1.40
additional information
not inhibited by 6-phosphogluconate, dithiothreitol or sodium tetrathionate; not inhibited by AMP, dithiothreitol or sodium tetrathionate
Arabidopsis thaliana
2.7.1.40
oxalate
0.2 mM, 50% of activity remaining; 0.2 mM, 71% of activity remaining, IC50: 0.41 mM
Arabidopsis thaliana
2.7.1.40
oxaloacetate
2 mM, 82% of activity remaining; 2 mM, 88% of activity remaining
Arabidopsis thaliana
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.40
plastid
-
Arabidopsis thaliana
9536
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.40
K+
strict requirement
Arabidopsis thaliana
2.7.1.40
Mg2+
strict requirement
Arabidopsis thaliana
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.40
57000
-
4 * 60000 + 4 * 57000, gel filtration
Arabidopsis thaliana
2.7.1.40
60000
-
4 * 60000 + 4 * 57000, gel filtration
Arabidopsis thaliana
2.7.1.40
463000
-
gel filtration
Arabidopsis thaliana
2.7.1.40
476000
-
gel filtration
Arabidopsis thaliana
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.40
Ni-NTA resin chromatography
Arabidopsis thaliana
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.40
seed
-
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.40
ADP + phosphoenolpyruvate
-
676434
Arabidopsis thaliana
ATP + pyruvate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.40
octamer
4 * 60000 + 4 * 57000, gel filtration
Arabidopsis thaliana
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.1.40
50
55
completely inactivated after 3 min of incubation at 50 to 55°C
Arabidopsis thaliana
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.40
8
-
-
Arabidopsis thaliana