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Prostaglandin-H-synthase (PGHS)-1 and -2 microtiter assays for the testing of herbal drugs and in vitro inhibition of PGHS-isoenzyms by polyunsaturated fatty acids from Platycodi radix

Reininger, E.A.; Bauer, R.; Phytomedicine 13, 164-169 (2006)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.99.1
alpha-linolenic acid
;
Ovis aries
1.14.99.1
DCM-extract of Angelicae dahuricae radix
0.1% inhibition of PGHS-1; 38.8% inhibition of PGHS-2
Ovis aries
1.14.99.1
DCM-extract of Angelicae sinsesis radix
55.8% inhibition of PGHS-2; 75.0% inhibition of PGHS-1
Ovis aries
1.14.99.1
DCM-extract of Atractylodis lanceae rhizoma
46.9% inhibition of PGHS-1; 50.3% inhibition of PGHS-2
Ovis aries
1.14.99.1
DCM-extract of Atractylodis macrocephalae rhizoma
47.0% inhibition of PGHS-2; 58.6% inhibition of PGHS-1
Ovis aries
1.14.99.1
DCM-extract of Cinnamomi ramulus
48.4% inhibition of PGHS-2; 73.5% inhibition of PGHS-1
Ovis aries
1.14.99.1
DCM-extract of Houttuyniae herba
40.9% inhibition of PGHS-2; 46.8% inhibition of PGHS-1
Ovis aries
1.14.99.1
DCM-extract of Notopterygii rhizoma seu radix
-2.1% inhibition of PGHS-2; 42.6% inhibition of PGHS-1
Ovis aries
1.14.99.1
DCM-extract of Piperis sarmentosi herba
10.1% inhibition of PGHS-2; 47.2% inhibition of PGHS-1
Ovis aries
1.14.99.1
DCM-extract of Platycodi radix
70.1% inhibition of PGHS-2; 77.8% inhibition of PGHS-1
Ovis aries
1.14.99.1
DCM-extract of Zanthoxyli pericarpium
18.3% inhibition of PGHS-1; 31.3% inhibition of PGHS-2
Ovis aries
1.14.99.1
DCM-extract of Zingiberis rhizoma
41.3% inhibition of PGHS-2; 52.9% inhibition of PGHS-1
Ovis aries
1.14.99.1
indomethacin
;
Ovis aries
1.14.99.1
linoleic acid
;
Ovis aries
1.14.99.1
n-hexane extract of Angelicae dahuricae radix
42.4% inhibition of PGHS-2; 52.5% inhibition of PGHS-1
Ovis aries
1.14.99.1
n-hexane extract of Angelicae sinsesis radix
61.5% inhibition of PGHS-2; 73.0% inhibition of PGHS-1
Ovis aries
1.14.99.1
n-hexane extract of Atractylodis lanceae rhizoma
67.4% inhibition of PGHS-1; 68.3% inhibition of PGHS-2
Ovis aries
1.14.99.1
n-hexane extract of Atractylodis macrocephalae rhizoma
46.1% inhibition of PGHS-1; 48.9% inhibition of PGHS-2
Ovis aries
1.14.99.1
n-hexane extract of Cinnamomi ramulus
23.6% inhibition of PGHS-2; 46.6% inhibition of PGHS-1
Ovis aries
1.14.99.1
n-hexane extract of Houttuyniae herba
43.4% inhibition of PGHS-2; 50.3% inhibition of PGHS-1
Ovis aries
1.14.99.1
n-hexane extract of Notopterygii rhizoma seu radix
64.9% inhibition of PGHS-2; 69.6% inhibition of PGHS-1
Ovis aries
1.14.99.1
n-hexane extract of Piperis sarmentosi herba
52.4% inhibition of PGHS-1; 65.0% inhibition of PGHS-2
Ovis aries
1.14.99.1
n-hexane extract of Platycodi radix
48.7% inhibition of PGHS-1; 55.1% inhibition of PGHS-2
Ovis aries
1.14.99.1
n-hexane extract of Zanthoxyli pericarpium
24.9% inhibition of PGHS-2; 48.5% inhibition of PGHS-1
Ovis aries
1.14.99.1
n-hexane extract of Zingiberis rhizoma
77.5% inhibition of PGHS-2; 83.4% inhibition of PGHS-1
Ovis aries
1.14.99.1
NS-398
;
Ovis aries
1.14.99.1
oleic acid
;
Ovis aries
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.99.1
arachidonate + AH2 + O2
Ovis aries
-
prostaglandin H2 + A + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.99.1
Ovis aries
P05979
PGHS-1
-
1.14.99.1
Ovis aries
P79208
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.99.1
seminal vesicle
PGHS-1
Ovis aries
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.99.1
arachidonate + AH2 + O2
-
676390
Ovis aries
prostaglandin H2 + A + H2O
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.99.1
22
-
activity assay at room temperature; activity assay at room temperature
Ovis aries
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.99.1
8
-
activity assay; activity assay
Ovis aries
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.99.1
Hematin
;
Ovis aries
1.14.99.1
additional information
epinephrine-hydrogentartrate can serve as cofactor; epinephrine-hydrogentartrate can servve as cofactor
Ovis aries
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
1.14.99.1
0.0008
-
inhibition of PGHS-2
Ovis aries
indomethacin
1.14.99.1
0.0009
-
inhibition of PGHS-1
Ovis aries
indomethacin
1.14.99.1
0.0026
-
inhibition of PGHS-2
Ovis aries
linoleic acid
1.14.99.1
0.0026
-
inhibition of PGHS-2
Ovis aries
NS-398
1.14.99.1
0.003
-
inhibition of PGHS-2
Ovis aries
alpha-linolenic acid
1.14.99.1
0.0198
-
inhibition of PGHS-1
Ovis aries
linoleic acid
1.14.99.1
0.0212
-
inhibition of PGHS-1
Ovis aries
alpha-linolenic acid
1.14.99.1
0.0507
-
inhibition of PGHS-1
Ovis aries
NS-398
1.14.99.1
0.1226
-
inhibition of PGHS-2
Ovis aries
oleic acid
1.14.99.1
0.1245
-
inhibition of PGHS-1
Ovis aries
oleic acid
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.99.1
Hematin
-
Ovis aries
1.14.99.1
additional information
epinephrine-hydrogentartrate can serve as cofactor
Ovis aries
1.14.99.1
additional information
epinephrine-hydrogentartrate can servve as cofactor
Ovis aries
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
1.14.99.1
0.0008
-
inhibition of PGHS-2
Ovis aries
indomethacin
1.14.99.1
0.0009
-
inhibition of PGHS-1
Ovis aries
indomethacin
1.14.99.1
0.0026
-
inhibition of PGHS-2
Ovis aries
linoleic acid
1.14.99.1
0.0026
-
inhibition of PGHS-2
Ovis aries
NS-398
1.14.99.1
0.003
-
inhibition of PGHS-2
Ovis aries
alpha-linolenic acid
1.14.99.1
0.0198
-
inhibition of PGHS-1
Ovis aries
linoleic acid
1.14.99.1
0.0212
-
inhibition of PGHS-1
Ovis aries
alpha-linolenic acid
1.14.99.1
0.0507
-
inhibition of PGHS-1
Ovis aries
NS-398
1.14.99.1
0.1226
-
inhibition of PGHS-2
Ovis aries
oleic acid
1.14.99.1
0.1245
-
inhibition of PGHS-1
Ovis aries
oleic acid
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.99.1
alpha-linolenic acid
-
Ovis aries
1.14.99.1
DCM-extract of Angelicae dahuricae radix
0.1% inhibition of PGHS-1
Ovis aries
1.14.99.1
DCM-extract of Angelicae dahuricae radix
38.8% inhibition of PGHS-2
Ovis aries
1.14.99.1
DCM-extract of Angelicae sinsesis radix
75.0% inhibition of PGHS-1
Ovis aries
1.14.99.1
DCM-extract of Angelicae sinsesis radix
55.8% inhibition of PGHS-2
Ovis aries
1.14.99.1
DCM-extract of Atractylodis lanceae rhizoma
46.9% inhibition of PGHS-1
Ovis aries
1.14.99.1
DCM-extract of Atractylodis lanceae rhizoma
50.3% inhibition of PGHS-2
Ovis aries
1.14.99.1
DCM-extract of Atractylodis macrocephalae rhizoma
58.6% inhibition of PGHS-1
Ovis aries
1.14.99.1
DCM-extract of Atractylodis macrocephalae rhizoma
47.0% inhibition of PGHS-2
Ovis aries
1.14.99.1
DCM-extract of Cinnamomi ramulus
73.5% inhibition of PGHS-1
Ovis aries
1.14.99.1
DCM-extract of Cinnamomi ramulus
48.4% inhibition of PGHS-2
Ovis aries
1.14.99.1
DCM-extract of Houttuyniae herba
46.8% inhibition of PGHS-1
Ovis aries
1.14.99.1
DCM-extract of Houttuyniae herba
40.9% inhibition of PGHS-2
Ovis aries
1.14.99.1
DCM-extract of Notopterygii rhizoma seu radix
42.6% inhibition of PGHS-1
Ovis aries
1.14.99.1
DCM-extract of Notopterygii rhizoma seu radix
-2.1% inhibition of PGHS-2
Ovis aries
1.14.99.1
DCM-extract of Piperis sarmentosi herba
47.2% inhibition of PGHS-1
Ovis aries
1.14.99.1
DCM-extract of Piperis sarmentosi herba
10.1% inhibition of PGHS-2
Ovis aries
1.14.99.1
DCM-extract of Platycodi radix
77.8% inhibition of PGHS-1
Ovis aries
1.14.99.1
DCM-extract of Platycodi radix
70.1% inhibition of PGHS-2
Ovis aries
1.14.99.1
DCM-extract of Zanthoxyli pericarpium
18.3% inhibition of PGHS-1
Ovis aries
1.14.99.1
DCM-extract of Zanthoxyli pericarpium
31.3% inhibition of PGHS-2
Ovis aries
1.14.99.1
DCM-extract of Zingiberis rhizoma
52.9% inhibition of PGHS-1
Ovis aries
1.14.99.1
DCM-extract of Zingiberis rhizoma
41.3% inhibition of PGHS-2
Ovis aries
1.14.99.1
indomethacin
-
Ovis aries
1.14.99.1
linoleic acid
-
Ovis aries
1.14.99.1
n-hexane extract of Angelicae dahuricae radix
52.5% inhibition of PGHS-1
Ovis aries
1.14.99.1
n-hexane extract of Angelicae dahuricae radix
42.4% inhibition of PGHS-2
Ovis aries
1.14.99.1
n-hexane extract of Angelicae sinsesis radix
73.0% inhibition of PGHS-1
Ovis aries
1.14.99.1
n-hexane extract of Angelicae sinsesis radix
61.5% inhibition of PGHS-2
Ovis aries
1.14.99.1
n-hexane extract of Atractylodis lanceae rhizoma
67.4% inhibition of PGHS-1
Ovis aries
1.14.99.1
n-hexane extract of Atractylodis lanceae rhizoma
68.3% inhibition of PGHS-2
Ovis aries
1.14.99.1
n-hexane extract of Atractylodis macrocephalae rhizoma
46.1% inhibition of PGHS-1
Ovis aries
1.14.99.1
n-hexane extract of Atractylodis macrocephalae rhizoma
48.9% inhibition of PGHS-2
Ovis aries
1.14.99.1
n-hexane extract of Cinnamomi ramulus
46.6% inhibition of PGHS-1
Ovis aries
1.14.99.1
n-hexane extract of Cinnamomi ramulus
23.6% inhibition of PGHS-2
Ovis aries
1.14.99.1
n-hexane extract of Houttuyniae herba
50.3% inhibition of PGHS-1
Ovis aries
1.14.99.1
n-hexane extract of Houttuyniae herba
43.4% inhibition of PGHS-2
Ovis aries
1.14.99.1
n-hexane extract of Notopterygii rhizoma seu radix
69.6% inhibition of PGHS-1
Ovis aries
1.14.99.1
n-hexane extract of Notopterygii rhizoma seu radix
64.9% inhibition of PGHS-2
Ovis aries
1.14.99.1
n-hexane extract of Piperis sarmentosi herba
52.4% inhibition of PGHS-1
Ovis aries
1.14.99.1
n-hexane extract of Piperis sarmentosi herba
65.0% inhibition of PGHS-2
Ovis aries
1.14.99.1
n-hexane extract of Platycodi radix
48.7% inhibition of PGHS-1
Ovis aries
1.14.99.1
n-hexane extract of Platycodi radix
55.1% inhibition of PGHS-2
Ovis aries
1.14.99.1
n-hexane extract of Zanthoxyli pericarpium
48.5% inhibition of PGHS-1
Ovis aries
1.14.99.1
n-hexane extract of Zanthoxyli pericarpium
24.9% inhibition of PGHS-2
Ovis aries
1.14.99.1
n-hexane extract of Zingiberis rhizoma
83.4% inhibition of PGHS-1
Ovis aries
1.14.99.1
n-hexane extract of Zingiberis rhizoma
77.5% inhibition of PGHS-2
Ovis aries
1.14.99.1
NS-398
-
Ovis aries
1.14.99.1
oleic acid
-
Ovis aries
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.99.1
arachidonate + AH2 + O2
Ovis aries
-
prostaglandin H2 + A + H2O
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.99.1
seminal vesicle
PGHS-1
Ovis aries
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.99.1
arachidonate + AH2 + O2
-
676390
Ovis aries
prostaglandin H2 + A + H2O
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.99.1
22
-
activity assay at room temperature
Ovis aries
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.99.1
8
-
activity assay
Ovis aries