BRENDA - Enzyme Database show

Characterization of peroxidase in buckwheat seed

Suzuki, T.; Honda, Y.; Mukasa, Y.; Kim, S.J.; Phytochemistry 67, 219-224 (2006)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.11.1.7
NH4+
0.18 mM, activation to 148% of control
Fagopyrum esculentum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.11.1.7
histidine
0.18 mM, 43% inhibition of the activity with quercetin, 13% inhibition of the activity with o-dianisidine, isoenzyme POX II; 0.18 mM, 71% inhibition of the activity with quercetin, 95% inhibition of the activity with o-dianisidine, isoenzyme POX I
Fagopyrum esculentum
1.11.1.7
imidazole
0.18 mM, 24% inhibition of the activity with quercetin, 81% inhibition of the activity with o-dianisidine, isoenzyme POX II; 0.18 mM, 72% inhibition of the activity with quercetin, 97% inhibition of the activity with o-dianisidine, isoenzyme POX I
Fagopyrum esculentum
1.11.1.7
linoleic acid
3.6 mM, 76% inhibition of the activity with quercetin, 48% inhibition of the activity with o-dianisidine, isoenzyme POX II; 3.6 mM, no inhibition of the activity with quercetin, 32% inhibition of the activity with o-dianisidine, isoenzyme POX I
Fagopyrum esculentum
1.11.1.7
linolenic acid
3.6 mM, 16% inhibition of the activity with quercetin, 21% inhibition of the activity with o-dianisidine, isoenzyme POX I; 3.6 mM, 35% inhibition of the activity with quercetin, 37% inhibition of the activity with o-dianisidine, isoenzyme POX II
Fagopyrum esculentum
1.11.1.7
NH4+
0.18 mM, 6% inhibition of the activity with quercetin; 0.18 mM, no inhibition of the activity with quercetin, 27% inhibition of the activity with o-dianisidine, isoenzyme POX I
Fagopyrum esculentum
1.11.1.7
oleic acid
3.6 mM, isoenzyme POX I, 62% inhibition of the activity with quercetin, 19% inhibition of the activity with o-dianisidine, isoenzyme POX I; 3.6 mM, isoenzyme POX II, 72% inhibition of the activity with quercetin, 41% inhibition of the activity with o-dianisidine, isoenzyme POX II
Fagopyrum esculentum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.11.1.7
0.016
-
reduced 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid)
isoenzyme POX II
Fagopyrum esculentum
1.11.1.7
0.028
-
quercetin
isoenzyme POX II
Fagopyrum esculentum
1.11.1.7
0.029
-
ascorbic acid
isoenzyme POX II
Fagopyrum esculentum
1.11.1.7
0.043
-
ascorbic acid
isoenzyme POX I
Fagopyrum esculentum
1.11.1.7
0.071
-
quercetin
isoenzyme POX I
Fagopyrum esculentum
1.11.1.7
0.137
-
o-Dianisidine
isoenzyme POX II
Fagopyrum esculentum
1.11.1.7
0.202
-
guaiacol
isoenzyme POX II
Fagopyrum esculentum
1.11.1.7
0.229
-
o-Dianisidine
isoenzyme POX I
Fagopyrum esculentum
1.11.1.7
0.288
-
guaiacol
isoenzyme POX I
Fagopyrum esculentum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.11.1.7
46000
-
isoenzyme POX I, gel filtration
Fagopyrum esculentum
1.11.1.7
58100
-
isoenzyme POX II, gel filtration
Fagopyrum esculentum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.11.1.7
Fagopyrum esculentum
-
Moench var. Kitawasesoba
-
Purification (Commentary)
EC Number
Commentary
Organism
1.11.1.7
isoenzyme POX I; isoenzyme POX II
Fagopyrum esculentum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.11.1.7
seed
isoenzyme POX I; isoenzyme POX II
Fagopyrum esculentum
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.11.1.7
ascorbic acid + H2O
isoenzyme POX I
676374
Fagopyrum esculentum
?
-
-
-
?
1.11.1.7
ascorbic acid + H2O
isoenzyme POX II
676374
Fagopyrum esculentum
?
-
-
-
?
1.11.1.7
guaiacol + H2O2
isoenzyme POX I
676374
Fagopyrum esculentum
?
-
-
-
?
1.11.1.7
guaiacol + H2O2
isoenzyme POX II
676374
Fagopyrum esculentum
?
-
-
-
?
1.11.1.7
additional information
isoenzyme POX I shows no activity with reduced 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid)
676374
Fagopyrum esculentum
?
-
-
-
-
1.11.1.7
o-dianisidine + H2O2
isoenzyme POX I
676374
Fagopyrum esculentum
?
-
-
-
?
1.11.1.7
o-dianisidine + H2O2
isoenzyme POX II
676374
Fagopyrum esculentum
?
-
-
-
?
1.11.1.7
quercetin + H2O
isoenzyme POX I
676374
Fagopyrum esculentum
?
-
-
-
?
1.11.1.7
quercetin + H2O
isoenzyme POX II
676374
Fagopyrum esculentum
?
-
-
-
?
1.11.1.7
reduced 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) + H2O2
isoenzyme POX II
676374
Fagopyrum esculentum
oxidized 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) + H2O
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.11.1.7
10
-
isoenzyme POX II
Fagopyrum esculentum
1.11.1.7
30
-
isoenzyme POX I
Fagopyrum esculentum
Temperature Range [C]
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
1.11.1.7
-
50
more than 50% of the activity of POX I is retained in the temperature range of 050C
Fagopyrum esculentum
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.11.1.7
-
30
isoenzyme POX I is stable
Fagopyrum esculentum
1.11.1.7
20
-
isoenzyme POX II is stable
Fagopyrum esculentum
1.11.1.7
30
-
isoenzyme POX II is unstable above
Fagopyrum esculentum
1.11.1.7
40
-
isoenzyme POX I is unstable
Fagopyrum esculentum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.11.1.7
3
-
activity with reduced 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid), isoenzyme POX II
Fagopyrum esculentum
1.11.1.7
4.5
-
activity with quercetin, isoenzyme POX II
Fagopyrum esculentum
1.11.1.7
5
-
activity with o-dianisidine, isoenzyme POX II
Fagopyrum esculentum
1.11.1.7
6
-
activity with o-dianisidine, isoenzyme POX I
Fagopyrum esculentum
1.11.1.7
7
-
activity with ascorbic acid, isoenzyme POX I; activity with ascorbic acid, isoenzyme POX II
Fagopyrum esculentum
1.11.1.7
8
-
activity with quercetin, isoenzyme POX I
Fagopyrum esculentum
1.11.1.7
9
-
activity with guaiacol, isoenzyme POX I; activity with guaiacol, isoenzyme POX II
Fagopyrum esculentum
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.11.1.7
NH4+
0.18 mM, activation to 148% of control
Fagopyrum esculentum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.11.1.7
histidine
0.18 mM, 43% inhibition of the activity with quercetin, 13% inhibition of the activity with o-dianisidine, isoenzyme POX II; 0.18 mM, 71% inhibition of the activity with quercetin, 95% inhibition of the activity with o-dianisidine, isoenzyme POX I
Fagopyrum esculentum
1.11.1.7
imidazole
0.18 mM, 24% inhibition of the activity with quercetin, 81% inhibition of the activity with o-dianisidine, isoenzyme POX II; 0.18 mM, 72% inhibition of the activity with quercetin, 97% inhibition of the activity with o-dianisidine, isoenzyme POX I
Fagopyrum esculentum
1.11.1.7
linoleic acid
3.6 mM, 76% inhibition of the activity with quercetin, 48% inhibition of the activity with o-dianisidine, isoenzyme POX II; 3.6 mM, no inhibition of the activity with quercetin, 32% inhibition of the activity with o-dianisidine, isoenzyme POX I
Fagopyrum esculentum
1.11.1.7
linolenic acid
3.6 mM, 16% inhibition of the activity with quercetin, 21% inhibition of the activity with o-dianisidine, isoenzyme POX I; 3.6 mM, 35% inhibition of the activity with quercetin, 37% inhibition of the activity with o-dianisidine, isoenzyme POX II
Fagopyrum esculentum
1.11.1.7
NH4+
0.18 mM, 6% inhibition of the activity with quercetin; 0.18 mM, no inhibition of the activity with quercetin, 27% inhibition of the activity with o-dianisidine, isoenzyme POX I
Fagopyrum esculentum
1.11.1.7
oleic acid
3.6 mM, isoenzyme POX I, 62% inhibition of the activity with quercetin, 19% inhibition of the activity with o-dianisidine, isoenzyme POX I; 3.6 mM, isoenzyme POX II, 72% inhibition of the activity with quercetin, 41% inhibition of the activity with o-dianisidine, isoenzyme POX II
Fagopyrum esculentum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.11.1.7
0.016
-
reduced 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid)
isoenzyme POX II
Fagopyrum esculentum
1.11.1.7
0.028
-
quercetin
isoenzyme POX II
Fagopyrum esculentum
1.11.1.7
0.029
-
ascorbic acid
isoenzyme POX II
Fagopyrum esculentum
1.11.1.7
0.043
-
ascorbic acid
isoenzyme POX I
Fagopyrum esculentum
1.11.1.7
0.071
-
quercetin
isoenzyme POX I
Fagopyrum esculentum
1.11.1.7
0.137
-
o-Dianisidine
isoenzyme POX II
Fagopyrum esculentum
1.11.1.7
0.202
-
guaiacol
isoenzyme POX II
Fagopyrum esculentum
1.11.1.7
0.229
-
o-Dianisidine
isoenzyme POX I
Fagopyrum esculentum
1.11.1.7
0.288
-
guaiacol
isoenzyme POX I
Fagopyrum esculentum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.11.1.7
46000
-
isoenzyme POX I, gel filtration
Fagopyrum esculentum
1.11.1.7
58100
-
isoenzyme POX II, gel filtration
Fagopyrum esculentum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.11.1.7
isoenzyme POX I; isoenzyme POX II
Fagopyrum esculentum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.11.1.7
seed
isoenzyme POX I; isoenzyme POX II
Fagopyrum esculentum
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.11.1.7
ascorbic acid + H2O
isoenzyme POX I
676374
Fagopyrum esculentum
?
-
-
-
?
1.11.1.7
ascorbic acid + H2O
isoenzyme POX II
676374
Fagopyrum esculentum
?
-
-
-
?
1.11.1.7
guaiacol + H2O2
isoenzyme POX I
676374
Fagopyrum esculentum
?
-
-
-
?
1.11.1.7
guaiacol + H2O2
isoenzyme POX II
676374
Fagopyrum esculentum
?
-
-
-
?
1.11.1.7
additional information
isoenzyme POX I shows no activity with reduced 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid)
676374
Fagopyrum esculentum
?
-
-
-
-
1.11.1.7
o-dianisidine + H2O2
isoenzyme POX I
676374
Fagopyrum esculentum
?
-
-
-
?
1.11.1.7
o-dianisidine + H2O2
isoenzyme POX II
676374
Fagopyrum esculentum
?
-
-
-
?
1.11.1.7
quercetin + H2O
isoenzyme POX I
676374
Fagopyrum esculentum
?
-
-
-
?
1.11.1.7
quercetin + H2O
isoenzyme POX II
676374
Fagopyrum esculentum
?
-
-
-
?
1.11.1.7
reduced 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) + H2O2
isoenzyme POX II
676374
Fagopyrum esculentum
oxidized 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) + H2O
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.11.1.7
10
-
isoenzyme POX II
Fagopyrum esculentum
1.11.1.7
30
-
isoenzyme POX I
Fagopyrum esculentum
Temperature Range [C] (protein specific)
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
1.11.1.7
-
50
more than 50% of the activity of POX I is retained in the temperature range of 050C
Fagopyrum esculentum
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.11.1.7
-
30
isoenzyme POX I is stable
Fagopyrum esculentum
1.11.1.7
20
-
isoenzyme POX II is stable
Fagopyrum esculentum
1.11.1.7
30
-
isoenzyme POX II is unstable above
Fagopyrum esculentum
1.11.1.7
40
-
isoenzyme POX I is unstable
Fagopyrum esculentum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.11.1.7
3
-
activity with reduced 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid), isoenzyme POX II
Fagopyrum esculentum
1.11.1.7
4.5
-
activity with quercetin, isoenzyme POX II
Fagopyrum esculentum
1.11.1.7
5
-
activity with o-dianisidine, isoenzyme POX II
Fagopyrum esculentum
1.11.1.7
6
-
activity with o-dianisidine, isoenzyme POX I
Fagopyrum esculentum
1.11.1.7
7
-
activity with ascorbic acid, isoenzyme POX I; activity with ascorbic acid, isoenzyme POX II
Fagopyrum esculentum
1.11.1.7
8
-
activity with quercetin, isoenzyme POX I
Fagopyrum esculentum
1.11.1.7
9
-
activity with guaiacol, isoenzyme POX I; activity with guaiacol, isoenzyme POX II
Fagopyrum esculentum