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Literature summary extracted from
- Characterization of peroxidase in buckwheat seed (2006), Phytochemistry, 67, 219-224.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.11.1.7 | NH4+ | 0.18 mM, activation to 148% of control | Fagopyrum esculentum |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.11.1.7 | histidine | 0.18 mM, 43% inhibition of the activity with quercetin, 13% inhibition of the activity with o-dianisidine, isoenzyme POX II; 0.18 mM, 71% inhibition of the activity with quercetin, 95% inhibition of the activity with o-dianisidine, isoenzyme POX I | Fagopyrum esculentum | |
| 1.11.1.7 | imidazole | 0.18 mM, 24% inhibition of the activity with quercetin, 81% inhibition of the activity with o-dianisidine, isoenzyme POX II; 0.18 mM, 72% inhibition of the activity with quercetin, 97% inhibition of the activity with o-dianisidine, isoenzyme POX I | Fagopyrum esculentum | |
| 1.11.1.7 | linoleic acid | 3.6 mM, 76% inhibition of the activity with quercetin, 48% inhibition of the activity with o-dianisidine, isoenzyme POX II; 3.6 mM, no inhibition of the activity with quercetin, 32% inhibition of the activity with o-dianisidine, isoenzyme POX I | Fagopyrum esculentum | |
| 1.11.1.7 | linolenic acid | 3.6 mM, 16% inhibition of the activity with quercetin, 21% inhibition of the activity with o-dianisidine, isoenzyme POX I; 3.6 mM, 35% inhibition of the activity with quercetin, 37% inhibition of the activity with o-dianisidine, isoenzyme POX II | Fagopyrum esculentum | |
| 1.11.1.7 | NH4+ | 0.18 mM, 6% inhibition of the activity with quercetin; 0.18 mM, no inhibition of the activity with quercetin, 27% inhibition of the activity with o-dianisidine, isoenzyme POX I | Fagopyrum esculentum | |
| 1.11.1.7 | oleic acid | 3.6 mM, isoenzyme POX I, 62% inhibition of the activity with quercetin, 19% inhibition of the activity with o-dianisidine, isoenzyme POX I; 3.6 mM, isoenzyme POX II, 72% inhibition of the activity with quercetin, 41% inhibition of the activity with o-dianisidine, isoenzyme POX II | Fagopyrum esculentum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.11.1.7 | 0.016 | - |
reduced 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) | isoenzyme POX II | Fagopyrum esculentum | |
| 1.11.1.7 | 0.028 | - |
quercetin | isoenzyme POX II | Fagopyrum esculentum | |
| 1.11.1.7 | 0.029 | - |
ascorbic acid | isoenzyme POX II | Fagopyrum esculentum | |
| 1.11.1.7 | 0.043 | - |
ascorbic acid | isoenzyme POX I | Fagopyrum esculentum | |
| 1.11.1.7 | 0.071 | - |
quercetin | isoenzyme POX I | Fagopyrum esculentum | |
| 1.11.1.7 | 0.137 | - |
o-Dianisidine | isoenzyme POX II | Fagopyrum esculentum | |
| 1.11.1.7 | 0.202 | - |
guaiacol | isoenzyme POX II | Fagopyrum esculentum | |
| 1.11.1.7 | 0.229 | - |
o-Dianisidine | isoenzyme POX I | Fagopyrum esculentum | |
| 1.11.1.7 | 0.288 | - |
guaiacol | isoenzyme POX I | Fagopyrum esculentum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.7 | 46000 | - |
isoenzyme POX I, gel filtration | Fagopyrum esculentum |
| 1.11.1.7 | 58100 | - |
isoenzyme POX II, gel filtration | Fagopyrum esculentum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.11.1.7 | Fagopyrum esculentum | - |
Moench var. Kitawasesoba | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.11.1.7 | isoenzyme POX I | Fagopyrum esculentum |
| 1.11.1.7 | isoenzyme POX II | Fagopyrum esculentum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.11.1.7 | seed | isoenzyme POX I | Fagopyrum esculentum | - |
| 1.11.1.7 | seed | isoenzyme POX II | Fagopyrum esculentum | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.11.1.7 | ascorbic acid + H2O | isoenzyme POX I | Fagopyrum esculentum | ? | - |
? | |
| 1.11.1.7 | ascorbic acid + H2O | isoenzyme POX II | Fagopyrum esculentum | ? | - |
? | |
| 1.11.1.7 | guaiacol + H2O2 | isoenzyme POX I | Fagopyrum esculentum | ? | - |
? | |
| 1.11.1.7 | guaiacol + H2O2 | isoenzyme POX II | Fagopyrum esculentum | ? | - |
? | |
| 1.11.1.7 | additional information | isoenzyme POX I shows no activity with reduced 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) | Fagopyrum esculentum | ? | - |
? | |
| 1.11.1.7 | o-dianisidine + H2O2 | isoenzyme POX I | Fagopyrum esculentum | ? | - |
? | |
| 1.11.1.7 | o-dianisidine + H2O2 | isoenzyme POX II | Fagopyrum esculentum | ? | - |
? | |
| 1.11.1.7 | quercetin + H2O | isoenzyme POX I | Fagopyrum esculentum | ? | - |
? | |
| 1.11.1.7 | quercetin + H2O | isoenzyme POX II | Fagopyrum esculentum | ? | - |
? | |
| 1.11.1.7 | reduced 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) + H2O2 | isoenzyme POX II | Fagopyrum esculentum | oxidized 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.11.1.7 | POX I | - |
Fagopyrum esculentum |
| 1.11.1.7 | POX II | - |
Fagopyrum esculentum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.7 | 10 | - |
isoenzyme POX II | Fagopyrum esculentum |
| 1.11.1.7 | 30 | - |
isoenzyme POX I | Fagopyrum esculentum |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.7 | - |
50 | more than 50% of the activity of POX I is retained in the temperature range of 050°C | Fagopyrum esculentum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.7 | - |
30 | isoenzyme POX I is stable | Fagopyrum esculentum |
| 1.11.1.7 | 20 | - |
isoenzyme POX II is stable | Fagopyrum esculentum |
| 1.11.1.7 | 30 | - |
isoenzyme POX II is unstable above | Fagopyrum esculentum |
| 1.11.1.7 | 40 | - |
isoenzyme POX I is unstable | Fagopyrum esculentum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.7 | 3 | - |
activity with reduced 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid), isoenzyme POX II | Fagopyrum esculentum |
| 1.11.1.7 | 4.5 | - |
activity with quercetin, isoenzyme POX II | Fagopyrum esculentum |
| 1.11.1.7 | 5 | - |
activity with o-dianisidine, isoenzyme POX II | Fagopyrum esculentum |
| 1.11.1.7 | 6 | - |
activity with o-dianisidine, isoenzyme POX I | Fagopyrum esculentum |
| 1.11.1.7 | 7 | - |
activity with ascorbic acid, isoenzyme POX I | Fagopyrum esculentum |
| 1.11.1.7 | 7 | - |
activity with ascorbic acid, isoenzyme POX II | Fagopyrum esculentum |
| 1.11.1.7 | 8 | - |
activity with quercetin, isoenzyme POX I | Fagopyrum esculentum |
| 1.11.1.7 | 9 | - |
activity with guaiacol, isoenzyme POX I | Fagopyrum esculentum |
| 1.11.1.7 | 9 | - |
activity with guaiacol, isoenzyme POX II | Fagopyrum esculentum |
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