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Literature summary extracted from

  • Yamamoto, H.; Yatou, A.; Inoue, K.
    8-dimethylallylnaringenin 2'-hydroxylase, the crucial cytochrome P450 mono-oxygenase for lavandulylated flavanone formation in Sophora flavescens cultured cells (2001), Phytochemistry, 58, 671-676.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.142 carbon monoxide
-
Sophora flavescens
1.14.14.142 cytochrome c 70% inhibition at 10 mM Sophora flavescens
1.14.14.142 Metyrapone 34% inhibition at 1 mM Sophora flavescens
1.14.14.142 miconazole 36% inhibition at 1 mM Sophora flavescens
1.14.14.142 additional information no or poor inhibition by ancymidol and KCN Sophora flavescens
1.14.14.142 tropolon 30% inhibition at 1 mM Sophora flavescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.142 0.055
-
8-dimethylallylnaringenin
-
Sophora flavescens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.142 membrane the enzyme is tightly bound to the membrane Sophora flavescens 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.142 8-dimethylallylnaringenin + [reduced NADPH-hemoprotein reductase] + O2 Sophora flavescens the enzyme is crucial for lavandulylated flavanone formation, the enzyme catalyzes the 2'-hydroxylation of 8-DMAN, which is then formed to sophoraflavanone G, pathway overview leachianone G + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.142 Sophora flavescens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.142 cell culture
-
Sophora flavescens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.142 (-)-(2S)-8-dimethylallylnaringenin + [oxidized NADPH-hemoprotein reductase] + H2O i.e. sophoraflavanone B, the enzyme catalyzes the 2'-hydroxylation of 8-DMAN and is O2-dependent Sophora flavescens leachianone G + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1.14.14.142 8-dimethylallylnaringenin + [reduced NADPH-hemoprotein reductase] + O2 the enzyme is crucial for lavandulylated flavanone formation, the enzyme catalyzes the 2'-hydroxylation of 8-DMAN, which is then formed to sophoraflavanone G, pathway overview Sophora flavescens leachianone G + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1.14.14.142 additional information the enzyme is a cytochrome P450 monooxygenase Sophora flavescens ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.142 8-DMAN 2'-hydroxylase
-
Sophora flavescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.142 30
-
assay at Sophora flavescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.142 8.5
-
-
Sophora flavescens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.142 additional information the enzyme is a cytochrome P450 monooxygenase Sophora flavescens