EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.142 | carbon monoxide | - |
Sophora flavescens | |
1.14.14.142 | cytochrome c | 70% inhibition at 10 mM | Sophora flavescens | |
1.14.14.142 | Metyrapone | 34% inhibition at 1 mM | Sophora flavescens | |
1.14.14.142 | miconazole | 36% inhibition at 1 mM | Sophora flavescens | |
1.14.14.142 | additional information | no or poor inhibition by ancymidol and KCN | Sophora flavescens | |
1.14.14.142 | tropolon | 30% inhibition at 1 mM | Sophora flavescens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.142 | 0.055 | - |
8-dimethylallylnaringenin | - |
Sophora flavescens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.14.142 | membrane | the enzyme is tightly bound to the membrane | Sophora flavescens | 16020 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.142 | 8-dimethylallylnaringenin + [reduced NADPH-hemoprotein reductase] + O2 | Sophora flavescens | the enzyme is crucial for lavandulylated flavanone formation, the enzyme catalyzes the 2'-hydroxylation of 8-DMAN, which is then formed to sophoraflavanone G, pathway overview | leachianone G + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.142 | Sophora flavescens | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.14.142 | cell culture | - |
Sophora flavescens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.142 | (-)-(2S)-8-dimethylallylnaringenin + [oxidized NADPH-hemoprotein reductase] + H2O | i.e. sophoraflavanone B, the enzyme catalyzes the 2'-hydroxylation of 8-DMAN and is O2-dependent | Sophora flavescens | leachianone G + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.142 | 8-dimethylallylnaringenin + [reduced NADPH-hemoprotein reductase] + O2 | the enzyme is crucial for lavandulylated flavanone formation, the enzyme catalyzes the 2'-hydroxylation of 8-DMAN, which is then formed to sophoraflavanone G, pathway overview | Sophora flavescens | leachianone G + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.142 | additional information | the enzyme is a cytochrome P450 monooxygenase | Sophora flavescens | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.142 | 8-DMAN 2'-hydroxylase | - |
Sophora flavescens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.14.142 | 30 | - |
assay at | Sophora flavescens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.14.142 | 8.5 | - |
- |
Sophora flavescens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.142 | additional information | the enzyme is a cytochrome P450 monooxygenase | Sophora flavescens |