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Literature summary extracted from

  • Almeida, M.G.; Humanes, M.; Melo, R.; Silva, J.A.; da Silva,J.J.; Wever, R.
    Purification and characterisation of vanadium haloperoxidases from the brown alga Pelvetia canaliculata (2005), Phytochemistry, 54, 5-11.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.11.1.B6 iodoperoxidase PcI is inactivated by extensive diafiltration using a Centricon-30 (Amicon) device against 100 mM citrate/phosphate pH 3.8 buffer in the presence of 1 mM EDTA, followed by a second diafiltration with 50 mM Tris-HCl (pH 9.0), reactivation by vanadium, iodoperoxidase PcI Pelvetia canaliculata
1.11.1.B6 iodoperoxidase PcII is inactivated by extensive diafiltration using a Centricon-30 (Amicon) device against 100 mM citrate/phosphate pH 3.8 buffer in the presence of 1 mM EDTA, followed by a second diafiltration with 50 mM Tris-HCl (pH 9.0), reactivation by vanadium, iodoperoxidase PcI Pelvetia canaliculata

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.B6 I- iodoperoxidases PcII Pelvetia canaliculata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.B6 0.11
-
 H2O2 iodoperoxidases PcI Pelvetia canaliculata
1.11.1.B6 0.2
-
 H2O2 iodoperoxidases PcII Pelvetia canaliculata
1.11.1.B6 2.1
-
 I- iodoperoxidases PcI Pelvetia canaliculata
1.11.1.B6 2.4
-
 I- iodoperoxidases PcII Pelvetia canaliculata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.B6 vanadate essential for catalytic activity, iodoperoxidases PcI Pelvetia canaliculata
1.11.1.B6 vanadate essential for catalytic activity, iodoperoxidases PcII Pelvetia canaliculata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.B6 additional information
-
-
 Pelvetia canaliculata
1.11.1.B6 66000
-
 x * 66000, iodoperoxidases PcI, SDS-PAGE Pelvetia canaliculata
1.11.1.B6 166000
-
 iodoperoxidases PcI, gel filtration Pelvetia canaliculata

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.B6 Pelvetia canaliculata
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.11.1.B6 glycoprotein iodoperoxidases PcI and iodoperoxidase PcII differ in their binding to ConA-Sepharose, which implies a different glycosylation pattern Pelvetia canaliculata

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.B6 iodoperoxidases PcI Pelvetia canaliculata
1.11.1.B6 iodoperoxidases PcII Pelvetia canaliculata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.B6 Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione i.e. monochlorodimedone, about 5% of the iodoperoxidase activity, iodoperoxidase PcI Pelvetia canaliculata ?
-
 ?
1.11.1.B6 Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione i.e. monochlorodimedone, about 5% of the iodoperoxidase activity, iodoperoxidase PcII Pelvetia canaliculata ?
-
 ?
1.11.1.B6 I- + H2O2 iodoperoxidase PcI show mainly iodoperoxidase activity, the specific activity of iodoperoxidase I is half of that of iodoperoxidase II Pelvetia canaliculata triiodide
-
 ?
1.11.1.B6 I- + H2O2 iodoperoxidase PcII shows mainly iodoperoxidase activity, the specific activity of iodoperoxidase I is half of that of iodoperoxidase II Pelvetia canaliculata triiodide
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.B6 ? x * 66000, iodoperoxidases PcI, SDS-PAGE Pelvetia canaliculata
1.11.1.B6 More
-
 Pelvetia canaliculata

Synonyms

EC Number Synonyms Comment Organism
1.11.1.B6 iodoperoxidases PcI
-
 Pelvetia canaliculata
1.11.1.B6 iodoperoxidases PcII
-
 Pelvetia canaliculata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.B6 40
-
 1 h, stable, iodoperoxidases PcI Pelvetia canaliculata
1.11.1.B6 40
-
 1 h, stable, iodoperoxidases PcII Pelvetia canaliculata
1.11.1.B6 50
-
 1 h, about 25% loss of activity, iodoperoxidases PcII Pelvetia canaliculata
1.11.1.B6 60
-
 1 h, about 50% loss of activity, iodoperoxidases PcII Pelvetia canaliculata
1.11.1.B6 60
-
 1 h, more than 90% loss of activity, iodoperoxidases PcI Pelvetia canaliculata
1.11.1.B6 70
-
 1 h, about 60% loss of activity, iodoperoxidase PcII Pelvetia canaliculata
1.11.1.B6 70
-
 1 h, complete inactivation, iodoperoxidases PcI Pelvetia canaliculata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.B6 6
-
 iodoperoxidases PcI Pelvetia canaliculata
1.11.1.B6 6.5
-
 iodoperoxidases PcII Pelvetia canaliculata

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.11.1.B6 0.06
-
 I- iodoperoxidases PcII Pelvetia canaliculata
1.11.1.B6 127
-
 I-
-
 Pelvetia canaliculata