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Literature summary extracted from

  • Jung, S.; Lee, Y.; Back, K.
    A tobacco plastidal transit sequence cannot override the dual targeting capacity of Myxococcus xanthus protoporphyrinogen oxidase in transgenic rice (2006), Pestic. Biochem. Physiol., 86, 49-56.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.3.3.4 additional information presence of the plastidal transit sequence neither excludes the intrinsic ability of subcellular translocation of Protox nor changes herbicide resistance in TTS lines Myxococcus xanthus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.3.4 full-length cDNA encoding Protox fused to a plastidal transit sequence at its N-terminus and subcloned into pGA1611 under the control of the constitutive maize ubiquitin promoter, with the hygromycin phosphotransferase gene as a selectable marker, overexpression in transgenic rice plants Myxococcus xanthus

Protein Variants

EC Number Protein Variants Comment Organism
1.3.3.4 additional information transgenic rice plant lines expressing Protox with and without the tobacco plastidal transit sequence, expression level in transgenic lines TTS3 and TTS4 are lower than that in transgenic line M4 Myxococcus xanthus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.3.4 oxyXuorfen transgenic rice lines TTS3 and TTS4 exhibit a reduced Protox activity, however, it is much greater than that of uninhibited Protox activity of wild type, seeds from all transgenic lines are able to germinate when treated with up to 0.5 mM, whereas seeds from the wild-type fail to germinate even when treated at levels as low as 0.001 mM, great accumulation of photodynamic protoporphyrin IX only in oxyfuorfen-treated wild-type plants, not in oxyfuorfen-treated TTS lines Myxococcus xanthus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.3.4 chloroplast prominent increase of Protox activity in transgenic rice plant lines TTS3 and TTS4 relative to the wild-type, increase is lower than that in transgenic line M4 Myxococcus xanthus 9507
-
1.3.3.4 mitochondrion prominent increase of Protox activity in transgenic rice plant lines TTS3 and TTS4 relative to the wild-type, increase is lower than that in transgenic line M4 Myxococcus xanthus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.3.4 48000
-
 immunoblot analysis, transgenic lines TTS3 and TTS4, presumably breakdown products of Protox Myxococcus xanthus
1.3.3.4 50000
-
 immunoblot analysis, transgenic lines TTS3 and TTS4 Myxococcus xanthus

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.4 Myxococcus xanthus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.4 protoporphyrinogen-IX + 3 O2
-
 Myxococcus xanthus protoporphyrin-IX + 3 H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.3.4 protox
-
 Myxococcus xanthus