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Literature summary extracted from

  • Yun, M.S.; Chen, W.; Deng, F.; Kiyokawa, T.; Mametsuka, K.; Yogo, Y.
    An in vitro screening assay to discover novel inhibitors of 4-coumarate:CoA ligase (2006), Pest Manag. Sci., 62, 1065-1071.
    View publication on PubMed

Application

EC Number Application Comment Organism
6.2.1.12 drug development the enzyme is a target for developing effective plant growth inhibitors, overview Nicotiana tabacum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.12 gene 4CL, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM105 Nicotiana tabacum

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.12 alachlor slight inhibition Nicotiana tabacum
6.2.1.12 bentazone slight inhibition Nicotiana tabacum
6.2.1.12 butachlor
-
 Nicotiana tabacum
6.2.1.12 chlornitrofen
-
 Nicotiana tabacum
6.2.1.12 dimepiperate
-
 Nicotiana tabacum
6.2.1.12 fenoxaprop-ethyl
-
 Nicotiana tabacum
6.2.1.12 linuron slight inhibition Nicotiana tabacum
6.2.1.12 mefenacet slight inhibition Nicotiana tabacum
6.2.1.12 molinate slight inhibition Nicotiana tabacum
6.2.1.12 additional information development and optimization of a high-throughput miniaturized screening system of plant growth inhibitors targeting 4CL in the phenylpropanoid pathway, in vitro inhibitor screening using 28 existing herbicides, overview, no inhibition by pendimethalin, trifluralin, alloxydim-sodium, bispyribac-sodium, dimethenamid, metolachlor, pyrazolate, bensulfuron-methyl, and imazosulfuron Nicotiana tabacum
6.2.1.12 nitrofen slight inhibition Nicotiana tabacum
6.2.1.12 Pentachlorophenol slight inhibition Nicotiana tabacum
6.2.1.12 pretilachlor slight inhibition Nicotiana tabacum
6.2.1.12 propanil 72% inhibition at 0.1 mM Nicotiana tabacum
6.2.1.12 pyrazosulfuron-ethyl slight inhibition Nicotiana tabacum
6.2.1.12 simazine slight inhibition Nicotiana tabacum
6.2.1.12 simetryn slight inhibition Nicotiana tabacum
6.2.1.12 swep 98% inhibition at 0.1 mM Nicotiana tabacum
6.2.1.12 thenylchlor
-
 Nicotiana tabacum
6.2.1.12 thiobencarb slight inhibition Nicotiana tabacum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.12 Mg2+
-
 Nicotiana tabacum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA Nicotiana tabacum the enzyme is involved in the phenylpropanoid pathway AMP + diphosphate + 4-coumaroyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.12 Nicotiana tabacum
-
 gene 4CL
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.12 recombinant enzyme 6.3fold from Escherichia coli strain JM105 by a multistep process involving anion exchange chromatography and gel filtration Nicotiana tabacum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.12 3.624
-
 purified recombinnat enzyme, substrate 4-coumaric acid Nicotiana tabacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA the enzyme is involved in the phenylpropanoid pathway Nicotiana tabacum AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA 4-hydroxycinnamic acid, 98%, predominantly trans-isomer Nicotiana tabacum AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + caffeic acid + CoA 3,4-dihydroxycinnamic acid, 98% Nicotiana tabacum AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + caffeic acid + CoA 3,4-dihydroxycinnamic acid, 98%, predominantly trans-isomer Nicotiana tabacum AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + cinnamic acid + CoA 3-phenyl-2-propenoic acid, 98% Nicotiana tabacum AMP + diphosphate + cinnamoyl-CoA
-
 ?
6.2.1.12 ATP + ferulic acid + CoA 4-hydroxy-3-methoxycinnamic acid, 99% Nicotiana tabacum AMP + diphosphate + 4-feruloyl-CoA
-
 ?
6.2.1.12 ATP + sinapic acid + CoA 3,5-dimethoxy-4-hydroxycinnamic acid, 98%, predominantly trans isomer Nicotiana tabacum AMP + diphosphate + 4-sinapoyl-CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.12 4-coumarate:CoA ligase
-
 Nicotiana tabacum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.12 30
-
 assay at Nicotiana tabacum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.12 7.8
-
 assay at Nicotiana tabacum

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.12 ATP
-
 Nicotiana tabacum