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Literature summary extracted from

  • Chemler, J.A.; Yan, Y.; Leonard, E.; Koffas, M.A.
    Combinatorial mutasynthesis of flavonoid analogues from acrylic acids in microorganisms (2007), Org. Lett., 9, 1855-1858.
    View publication on PubMed

Application

EC Number Application Comment Organism
5.5.1.6 synthesis combinatorial expression of 4-coumaroyl:Co A ligase, chalcone synthase, chalcone isomerase, and flavanone 3beta-hydrolase in Saccharomyces cerevisiae for production of unnatural flavanones when fed with aromatic acrylic acids Scutellaria baicalensis
6.2.1.12 biotechnology viability of a flavonoid network to utilize acrylic acid analogues and describe the combinatorial mutasynthesis of novel unnatural flavonoids using recombinant Saccharomyces cerevisiae, overview Saccharomyces cerevisiae
6.2.1.12 synthesis viability of a flavonoid network to utilize acrylic acid analogues and describe the combinatorial mutasynthesis of novel unnatural flavonoids using recombinant Saccharomyces cerevisiae, overview Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.5.1.6 combinatorial expression of 4-coumaroyl:Co A ligase, chalcone synthase, chalcone isomerase, and flavanone 3beta-hydrolase in Saccharomyces cerevisiae Scutellaria baicalensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.12 Mg2+
-
 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA Saccharomyces cerevisiae the enzyme is involved in the phenylpropanoid pathway AMP + diphosphate + 4-coumaroyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.5.1.6 Scutellaria baicalensis
-
-
-
6.2.1.12 Saccharomyces cerevisiae
-
 gene 4CL
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.5.1.6 (2E)-3-(2-fluorophenyl)-1-(2,4,6-trihydroxyphenyl)prop-2-en-1-one based on substrate (2E)-3-(2-fluorophenyl)acrylic acid in combinatorial expression system of 4-coumaroyl:Co A ligase, chalcone synthase, chalcone isomerase, and flavanone 3beta-hydrolase Scutellaria baicalensis (2R)-2-(2-fluorophenyl)-5,7-dihydroxy-2,3-dihydro-4H-chromen-4-one
-
 ?
5.5.1.6 (2E)-3-(2-hydroxyphenyl)-1-(2,4,6-trihydroxyphenyl)prop-2-en-1-one based on substrate (2E)-3-(2-hydroxyphenyl)acrylic acid in combinatorial expression system of 4-coumaroyl:Co A ligase, chalcone synthase, chalcone isomerase, and flavanone 3beta-hydrolase Scutellaria baicalensis (2R)-5,7-dihydroxy-2-(2-hydroxyphenyl)-2,3-dihydro-4H-chromen-4-one
-
 ?
5.5.1.6 (2E)-3-(3-hydroxyphenyl)-1-(2,4,6-trihydroxyphenyl)prop-2-en-1-one based on substrate (2E)-3-(3-hydroxyphenyl)acrylic acid in combinatorial expression system of 4-coumaroyl:Co A ligase, chalcone synthase, chalcone isomerase, and flavanone 3beta-hydrolase Scutellaria baicalensis (2R)-5,7-dihydroxy-2-(3-hydroxyphenyl)-2,3-dihydro-4H-chromen-4-one
-
 ?
5.5.1.6 (2E)-3-(4-aminophenyl)-1-(2,4,6-trihydroxyphenyl)prop-2-en-1-one based on substrate (2E)-3-(4-aminophenyl)acrylic acid in combinatorial expression system of 4-coumaroyl:Co A ligase, chalcone synthase, chalcone isomerase, and flavanone 3beta-hydrolase Scutellaria baicalensis (2R)-2-(4-aminophenyl)-5,7-dihydroxy-2,3-dihydro-4H-chromen-4-one
-
 ?
5.5.1.6 (2E)-3-(4-fluorophenyl)-1-(2,4,6-trihydroxyphenyl)prop-2-en-1-one based on substrate (2E)-3-(4-fluorophenyl)acrylic acid in combinatorial expression system of 4-coumaroyl:Co A ligase, chalcone synthase, chalcone isomerase, and flavanone 3beta-hydrolase Scutellaria baicalensis (2R)-2-(4-fluorophenyl)-5,7-dihydroxy-2,3-dihydro-4H-chromen-4-one
-
 ?
5.5.1.6 (2E)-3-(4-hydroxyphenyl)-1-(2,4,6-trihydroxyphenyl)prop-2-en-1-one based on substrate (2E)-3-(4-hydroxyphenyl)acrylic acid in combinatorial expression system of 4-coumaroyl:Co A ligase, chalcone synthase, chalcone isomerase, and flavanone 3beta-hydrolase Scutellaria baicalensis (2S)-5,7-dihydroxy-2-(4-hydroxyphenyl)-2,3-dihydro-4H-chromen-4-one
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA
-
 Saccharomyces cerevisiae AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA the enzyme is involved in the phenylpropanoid pathway Saccharomyces cerevisiae AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 additional information the enzyme performs synthesis of (5,7-dihydroxy-4-chromanone)-propenoic acid in concert with chalcone synthase, chalcone isomerase, and flavanone 3‚-hydroxylase, fed with a number of aromatic acrylic acids, mechanism, overview Saccharomyces cerevisiae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.12 4-coumaroyl:CoA ligase
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.12 ATP
-
 Saccharomyces cerevisiae