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Literature summary extracted from

  • Mihovilovic, M.D.; Rudroff, F.; Winninger, A.; Schneider, T.; Schulz, F.; Reetz, M.T.
    Microbial Baeyer-Villiger oxidation: stereopreference and substrate acceptance of cyclohexanone monooxygenase mutants prepared by directed evolution (2006), Org. Lett., 8, 1221-1224.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.22 overexpression of wild-type and mutants in Escherichia coli Acinetobacter sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.22 F432I random mutagenesis, the mutant shows altered substrate specificity and increased enantioselectivity compared to the wild-type enzyme Acinetobacter sp.
1.14.13.22 F432S random mutagenesis, the mutant shows altered substrate specificity and increased enantioselectivity compared to the wild-type enzyme Acinetobacter sp.
1.14.13.22 F432Y/K500R random mutagenesis, the mutant shows altered substrate specificity and increased enantioselectivity compared to the wild-type enzyme Acinetobacter sp.
1.14.13.22 K78E/F432S random mutagenesis, the mutant shows altered substrate specificity and increased enantioselectivity compared to the wild-type enzyme Acinetobacter sp.
1.14.13.22 L426P/A541V random mutagenesis, the mutant shows altered substrate specificity and increased enantioselectivity compared to the wild-type enzyme Acinetobacter sp.
1.14.13.22 additional information screening of random mutants of the enzyme against a library of structurally diverse ketones for modifications in the substrate acceptance profile and stereopreference of the enzymatic Baeyer-Villiger biooxidation, improved and/or divergent stereoselectivities are observed for several substrates, overview Acinetobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.22 4-hydroxy-cyclohexanone + NADPH + O2 Acinetobacter sp.
-
 4-hydroxy-hexano-6-lactone + NADP+ + H2O
-
 ?
1.14.13.22 4-hydroxy-cyclohexanone + NADPH + O2 Acinetobacter sp. NCIMB 9871
-
 4-hydroxy-hexano-6-lactone + NADP+ + H2O
-
 ?
1.14.13.22 cyclohexanone + NADPH + O2 Acinetobacter sp.
-
 hexano-6-lactone + NADP+ + H2O
-
 ?
1.14.13.22 cyclohexanone + NADPH + O2 Acinetobacter sp. NCIMB 9871
-
 hexano-6-lactone + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.22 Acinetobacter sp.
-
-
-
1.14.13.22 Acinetobacter sp. NCIMB 9871
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.22 4-hydroxy-cyclohexanone + NADPH + O2
-
 Acinetobacter sp. 4-hydroxy-hexano-6-lactone + NADP+ + H2O
-
 ?
1.14.13.22 4-hydroxy-cyclohexanone + NADPH + O2 an enantioselective Baeyer-Villiger oxidation Acinetobacter sp. 4-hydroxy-hexano-6-lactone + NADP+ + H2O
-
 ?
1.14.13.22 4-hydroxy-cyclohexanone + NADPH + O2
-
 Acinetobacter sp. NCIMB 9871 4-hydroxy-hexano-6-lactone + NADP+ + H2O
-
 ?
1.14.13.22 4-hydroxy-cyclohexanone + NADPH + O2 an enantioselective Baeyer-Villiger oxidation Acinetobacter sp. NCIMB 9871 4-hydroxy-hexano-6-lactone + NADP+ + H2O
-
 ?
1.14.13.22 cyclohexanone + NADPH + O2
-
 Acinetobacter sp. hexano-6-lactone + NADP+ + H2O
-
 ?
1.14.13.22 cyclohexanone + NADPH + O2
-
 Acinetobacter sp. NCIMB 9871 hexano-6-lactone + NADP+ + H2O
-
 ?
1.14.13.22 additional information substrate specificity and enantioselectivity of wild-type and mutant enzymes, overview Acinetobacter sp. ?
-
 ?
1.14.13.22 additional information substrate specificity and enantioselectivity of wild-type and mutant enzymes, overview Acinetobacter sp. NCIMB 9871 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.22 CHMO
-
 Acinetobacter sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.22 NADPH
-
 Acinetobacter sp.