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Literature summary extracted from

  • Cobb, S.L.; Deng, H.; McEwan, A.R.; Naismith, J.H.; OHagan, D.; Robinson, D.A.
    Substrate specificity in enzymatic fluorination. The fluorinase from Streptomyces cattleya accepts 2-deoxyadenosine substrates (2006), Org. Biomol. Chem., 4, 1458-1460.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.63 purified recombinant enzyme, free from tags or bound adenosine, complexed to 2'-deoxy-5'-fluoroadenosine, 4 mg/ml protein is incubated with 20 mM ligand at 25°C for 4 h, followed by crystallization using vapour diffusion against a reservoir containing 30% PEG 1000, 0.1 M phosphate-citrate pH 4.5, 0.2 M Li2SO4, X-ray diffraction structure determination and analysis at 2.4 A resolution, molecular replacement Streptomyces cattleya

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.63 S-adenosyl-L-methionine + fluoride Streptomyces cattleya first committed enzymatic step on the biosynthetic pathway to the fluorometabolites fluoroacetate and 4-fluorothreonine 5'-deoxy-5'-fluoroadenosine + L-methionine
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 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.63 Streptomyces cattleya
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-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.63 2'-deoxyadenosyl-L-methionine + fluoride C-F bond formation, substrate binding structure, NMR and mass spectrometry, overview Streptomyces cattleya 2'-deoxy-5'-fluoroadenosine + L-methionine in the reverse reaction the enzyme shows 10% activity with the 2'-deoxy compound compared to the activity with the 5'-derivative r
2.5.1.63 additional information substrate specificity, the enzyme does not require a planar ribose conformation of the substrate to catalyse C-F bond formation, overview, the enzyme catalyzes also transhalogenation from 2'-deoxy-5'-chloroadenosine to 2'-deoxy-fluoroadenosine via 2'-deoxy-SeAM, overview Streptomyces cattleya ?
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 ?
2.5.1.63 S-adenosyl-L-methionine + fluoride first committed enzymatic step on the biosynthetic pathway to the fluorometabolites fluoroacetate and 4-fluorothreonine Streptomyces cattleya 5'-deoxy-5'-fluoroadenosine + L-methionine
-
 ?
2.5.1.63 S-adenosyl-L-methionine + fluoride C-F bond formation, substrate binding structure, NMR and mass spectrometry, overview Streptomyces cattleya 5'-deoxy-5'-fluoroadenosine + L-methionine
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 r

Synonyms

EC Number Synonyms Comment Organism
2.5.1.63 fluorinase
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 Streptomyces cattleya