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Literature summary extracted from

  • Speare, D.M.; Fleming, S.M.; Beckett, M.N.; Li, J.J.; Bugg, T.D.
    Synthetic 6-aryl-2-hydroxy-6-ketohexa-2,4-dienoic acid substrates for C-C hydrolase BphD: investigation of a general base catalytic mechanism (2004), Org. Biomol. Chem., 2, 2942-2950.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.7.1.8 overexpression in Escherichia coli BL21(DE3)(pLysS1)/pAIA51 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7.1.8 additional information
-
 additional information values between 0.00013 and 0.00106 mM for processing of the 6-acryl-2-hydroxy-6-ketohexa-2,4-dienoic acid substrates Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.8 Pseudomonas sp.
-
 LB400
-
3.7.1.8 Pseudomonas sp. LB400
-
 LB400
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.7.1.8 to more than 90% homogeneitiy Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.7.1.8 6.4
-
 purified enzyme Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.8 2,6-dihydroxy-6-phenylhexa-2,4-dienoate
-
 Pseudomonas sp. benzaldehyde + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
3.7.1.8 2,6-dihydroxy-6-phenylhexa-2,4-dienoate
-
 Pseudomonas sp. LB400 benzaldehyde + (2E)-2-hydroxypenta-2,4-dienoic acid
-
 ?
3.7.1.8 additional information 6-acryl-2-hydroxy-6-ketohexa-2,4-dienoic acids synthesized as substrates for C-C hydrolase BphD Pseudomonas sp. ?
-
 ?
3.7.1.8 additional information 6-acryl-2-hydroxy-6-ketohexa-2,4-dienoic acids synthesized as substrates for C-C hydrolase BphD Pseudomonas sp. LB400 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.7.1.8 BphD
-
 Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.7.1.8 additional information
-
 additional information values between 0.88 and 5.85 s-1 for processing of the 6-acryl-2-hydroxy-6-ketohexa-2,4-dienoic acid substrates Pseudomonas sp.