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Literature summary extracted from

  • Taomoto, J.; Yoshida, K.; Wada, Y.; Tanabe, K.; Konishi, K.; Tahara, H.; Fukushima, M.
    Overexpression of the orotate phosphoribosyl-transferase gene enhances the effect of 5-fluorouracil on gastric cancer cell lines (2006), Oncology, 70, 458-464.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.10 OPRT, stable overexpression in the gastric cancer cell lines TMK-1 and MKN-45, enhancing the effect of 5-fluorouracil on the cells, transfected cells show a similar growth curve as the wild-type parent cells, while the drug sensitivity is increased compared to the untransfected cells, overview Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.10 52000
-
 x * 52000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.10 5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate Homo sapiens
-
 5-fluorouridine 5'-phosphate + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.10 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.10 gastric cancer cell
-
 Homo sapiens
-
2.4.2.10 MKN-45 cell gastric cancer cell line with low baseline expression levels of OPRT Homo sapiens
-
2.4.2.10 TMK-1 cell gastric cancer cell line with low baseline expression levels of OPRT Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.10 0.0000045
-
 untransfected TMK-1 cells Homo sapiens
2.4.2.10 0.000009
-
 untransfected MKN-45 cells Homo sapiens
2.4.2.10 0.000072
-
 transfected MKN-45-OPRT cells Homo sapiens
2.4.2.10 0.000172
-
 transfected TMK-OPRT cells Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.10 5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Homo sapiens 5-fluorouridine 5'-phosphate + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.10 ? x * 52000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.4.2.10 OPRT
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.10 7.4
-
 assay at Homo sapiens