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Literature summary extracted from

  • Alexandre, J.A.; Roy, B.; Topalis, D.; Pochet, S.; Perigaud, C.; Deville-Bonne, D.
    Enantioselectivity of human AMP, dTMP and UMP-CMP kinases (2007), Nucleic Acids Res., 35, 4895-4904.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.14 overexpression in Escherichia coli Homo sapiens
2.7.4.25 overexpression in Escherichia coli Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.4.25 of the R188M mutant alone or with dCMP Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.25 D132A site directed mutagenesis Escherichia coli
2.7.4.25 D132H site directed mutagenesis Escherichia coli
2.7.4.25 D132N site directed mutagenesis Escherichia coli
2.7.4.25 D132S site directed mutagenesis Escherichia coli
2.7.4.25 R110M site directed mutagenesis Escherichia coli
2.7.4.25 R188M site directed mutagenesis Escherichia coli
2.7.4.25 S36A site directed mutagenesis Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.14 L-CMP excess of L-CMP Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.9 0.02
-
 D-dTMP
-
 Homo sapiens
2.7.4.9 0.38
-
 L-dTMP
-
 Homo sapiens
2.7.4.14 0.02
-
 D-CMP
-
 Homo sapiens
2.7.4.14 0.05
-
 D-UMP
-
 Homo sapiens
2.7.4.14 0.15
-
 L-3TCMP
-
 Homo sapiens
2.7.4.14 0.36
-
 beta-D-2'-azido-2'-dCMP
-
 Homo sapiens
2.7.4.14 0.7
-
 L-dUMP L-enantiomers have lower reaction rates Homo sapiens
2.7.4.14 0.73
-
 L-dCMP L-enantiomers have lower reaction rates Homo sapiens
2.7.4.14 0.75
-
 L-CMP
-
 Homo sapiens
2.7.4.14 0.9
-
 beta-D-2'-azido-2'-dUMP
-
 Homo sapiens
2.7.4.14 1
-
 D-dCMP
-
 Homo sapiens
2.7.4.14 1.2
-
 beta-L-2'-azido-2'-dCMP
-
 Homo sapiens
2.7.4.14 1.3
-
 D-dUMP
-
 Homo sapiens
2.7.4.14 1.3
-
 beta-L-2'-azido-2'-dUMP
-
 Homo sapiens
2.7.4.25 0.035
-
 CMP wild type Escherichia coli
2.7.4.25 0.038
-
 CMP D132S mutant Escherichia coli
2.7.4.25 0.055
-
 dCMP D132H mutant Escherichia coli
2.7.4.25 0.08
-
 dCMP D132N mutant Escherichia coli
2.7.4.25 0.09
-
 dCMP D132S mutant Escherichia coli
2.7.4.25 0.094
-
 dCMP wild type Escherichia coli
2.7.4.25 0.77
-
 dCMP R110M mutant Escherichia coli
2.7.4.25 0.93
-
 UMP wild type Escherichia coli
2.7.4.25 1
-
 CMP R110M mutant Escherichia coli
2.7.4.25 1.3
-
 CMP D132H mutant Escherichia coli
2.7.4.25 1.8
-
 dCMP D132A mutant Escherichia coli
2.7.4.25 1.9
-
 UMP S36A mutant Escherichia coli
2.7.4.25 2.5
-
 CMP S36A mutant Escherichia coli
2.7.4.25 2.6
-
 CMP D132N mutant Escherichia coli
2.7.4.25 2.9
-
 CMP D132A mutant Escherichia coli
2.7.4.25 3.5
-
 dCMP S36A mutant Escherichia coli
2.7.4.25 3.9
-
 UMP D132H mutant Escherichia coli
2.7.4.25 4.5
-
 UMP D132N mutant Escherichia coli
2.7.4.25 7.3
-
 dCMP R110M mutant Escherichia coli
2.7.4.25 7.9
-
 UMP D132A mutant Escherichia coli
2.7.4.25 8
-
 UMP D132S mutant Escherichia coli
2.7.4.25 11.3
-
 UMP R110M mutant Escherichia coli
2.7.4.25 20.2
-
 CMP R110M mutant Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.9 L-dTMP + ATP Homo sapiens
-
 L-dTDP + ADP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.9 Homo sapiens P23919
-
-
2.7.4.14 Homo sapiens P30085
-
-
2.7.4.25 Escherichia coli P0A6I0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.14 of the recombinant proteins Homo sapiens
2.7.4.25 of the recombinant proteins Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.9 D-dTMP + ATP
-
 Homo sapiens D-dTDP + ADP
-
 ?
2.7.4.9 L-dTMP + ATP
-
 Homo sapiens L-dTDP + ADP
-
 ?
2.7.4.14 ATP + beta-D-2'-azido-2'-dCMP
-
 Homo sapiens ADP + beta-D-2'-azido-2'-dCDP
-
 ?
2.7.4.14 ATP + beta-D-2'-azido-2'-dUMP
-
 Homo sapiens ADP + beta-D-2'-azido-2'-dUDP
-
 ?
2.7.4.14 ATP + beta-L-2'-azido-2'-dCMP
-
 Homo sapiens ADP + beta-L-2'-azido-2'-dCDP
-
 ?
2.7.4.14 ATP + beta-L-2'-azido-2'-dUMP
-
 Homo sapiens ADP + beta-L-2'-azido-2'-dUDP
-
 ?
2.7.4.14 ATP + D-CMP
-
 Homo sapiens ADP + D-CDP
-
 ?
2.7.4.14 ATP + D-dCMP
-
 Homo sapiens ADP + D-dCDP
-
 ?
2.7.4.14 ATP + D-dUMP
-
 Homo sapiens ADP + D-dUDP
-
 ?
2.7.4.14 ATP + D-UMP
-
 Homo sapiens ADP + D-UDP
-
 ?
2.7.4.14 ATP + L-3TCMP
-
 Homo sapiens ADP + L-3TCMP
-
 ?
2.7.4.14 ATP + L-CMP poor substrate Homo sapiens ADP + L-CDP
-
 ?
2.7.4.14 ATP + L-dCMP slower phosphorylation than the D-enantiomers Homo sapiens ADP + L-dCDP
-
 ?
2.7.4.14 ATP + L-dUMP slower phosphorylation than the D-enantiomers Homo sapiens ADP + L-dUDP
-
 ?
2.7.4.25 ATP + CMP
-
 Escherichia coli ADP + CDP
-
 ?
2.7.4.25 ATP + dCMP
-
 Escherichia coli ADP + dCDP
-
 ?
2.7.4.25 ATP + UMP
-
 Escherichia coli ADP + UDP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.4.9 dTMP kinases
-
 Homo sapiens
2.7.4.9 human thymidine monophosphate kinase (hTMPK)
-
 Homo sapiens
2.7.4.9 thymidylate kinase
-
 Homo sapiens
2.7.4.14 cytidylate kinase
-
 Homo sapiens
2.7.4.14 UCK
-
 Homo sapiens
2.7.4.14 UMP-CMP kinase
-
 Homo sapiens
2.7.4.25 CMP kinase
-
 Escherichia coli
2.7.4.25 cytidylate kinase
-
 Escherichia coli
2.7.4.25 UMP-CMP kinase
-
 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.25 43
-
 mutant D132A Escherichia coli
2.7.4.25 45
-
 mutant R110M Escherichia coli
2.7.4.25 47
-
 mutant D132N Escherichia coli
2.7.4.25 48
-
 mutant D132S, mutant D132H Escherichia coli
2.7.4.25 51
-
 mutant R188M Escherichia coli
2.7.4.25 52
-
 wild type, mutant S36A Escherichia coli