EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.74 | mutant protein expressed in Escherichia coli | Homo sapiens |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.7.1.74 | hanging drop vapour diffusion method, mutant enzyme crystallized in complex with its substrates (-)-beta-2,3-dideoxy-3-thiacytidine and troxacitabine | Homo sapiens |
2.7.1.74 | mutant C9S/C45S/C59S/C146S in complex with L-nucleosides (-)-beta-2',3'-dideoxy-3'-thiacytidine and troxacitabine. The nucleoside binding-site tolerates substrates with different chiral configurations by maintaining virtually all of the protein-ligand interactions responsible for productive substrate positioning. The pseudo-symmetry of nucleosides and nucleoside analogs in combination with their conformational flexibility allows the L- and D-enantiomeric forms to adopt similar shapes when bound to the enzyme | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.1.74 | C9S/C45S/C59S/C146S | engineered protein has a significantly improved crystallization behaviour compared with the wild type protein | Homo sapiens |
2.7.1.74 | C9S/C45S/C59S/C146S | mutation of the four surface-exposed Cys residues, constructued for crystallization. Mutant displays moderate kinetic differences in the catalytic efficiency when compared with wild-type | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.74 | 0.003 | - |
(-)-beta-2',3'-dideoxy-3'-thiacytidine | wild type enzyme with ATP as phosphate donor, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.0034 | - |
ATP | cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, wild-type, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.008 | - |
(-)-beta-2',3'-dideoxy-3'-thiacytidine | C9S/C45S/C59S/C146S mutant enzyme with ATP as phosphate donor, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.008 | - |
(-)-beta-2',3'-dideoxy-3'-thiacytidine | wild type enzyme with UTP as phosphate donor, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.008 | - |
UTP | cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, wild-type, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.0084 | - |
ATP | cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.0106 | - |
UTP | cosubstrate troxacitabine, wild-type, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.011 | - |
troxacitabine | wild type enzyme with UTP as phosphate donor, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.013 | - |
troxacitabine | wild type enzyme with ATP as phosphate donor, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.0132 | - |
ATP | cosubstrate troxacitabine, wild-type, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.0149 | - |
UTP | cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.015 | - |
(-)-beta-2',3'-dideoxy-3'-thiacytidine | C9S/C45S/C59S/C146S mutant enzyme with UTP as phosphate donor, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.0568 | - |
ATP | cosubstrate troxacitabine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.057 | - |
troxacitabine | C9S/C45S/C59S/C146S mutant enzyme with ATP as phosphate donor, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.0578 | - |
UTP | cosubstrate troxacitabine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.058 | - |
troxacitabine | C9S/C45S/C59S/C146Smutant enzyme with UTP as phosphate donor, 37°C, pH 7.5 | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.74 | Mg2+ | in complex with ATP or UTP | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.74 | additional information | Homo sapiens | dCK is able to phosphorylate both D- and L-nucleosides and nucleoside analogs | ? | - |
? | |
2.7.1.74 | NTP + (-)-beta-2',3'-dideoxy-3'-thiacytidine | Homo sapiens | activation of the clinically relevant substrate | NDP + (-)-beta-2',3'-dideoxy-3'-thiacytidine 5'-phosphate | - |
? | |
2.7.1.74 | NTP + troxacitabine | Homo sapiens | activation of the clinically relevant substrate | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.74 | Homo sapiens | - |
- |
- |
2.7.1.74 | Homo sapiens | P27707 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.74 | recombinant protein from Escherichia coli | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.74 | ATP + (-)-beta-2',3'-dideoxy-3'-thiacytidine | - |
Homo sapiens | ADP + (-)-beta-2',3'-dideoxy-3'-thiacytidine 5'-phosphate | - |
? | |
2.7.1.74 | ATP + (-)-beta-2',3'-dideoxy-3'-thiacytidine | i.e. lamivudine | Homo sapiens | ADP + (-)-beta-2',3'-dideoxy-3'-thiacytidine 5'-phosphate | - |
? | |
2.7.1.74 | ATP + D-2'-deoxycytidine | - |
Homo sapiens | ADP + D-2'-deoxycytidine 5'-phosphate | - |
? | |
2.7.1.74 | ATP + L-2'-deoxycytidine | - |
Homo sapiens | ADP + L-2'-deoxycytidine 5'-phosphate | - |
? | |
2.7.1.74 | ATP + troxacitabine | - |
Homo sapiens | ADP + troxacitabine 5'-phosphate | - |
? | |
2.7.1.74 | additional information | dCK is able to phosphorylate both D- and L-nucleosides and nucleoside analogs | Homo sapiens | ? | - |
? | |
2.7.1.74 | NTP + (-)-beta-2',3'-dideoxy-3'-thiacytidine | activation of the clinically relevant substrate | Homo sapiens | NDP + (-)-beta-2',3'-dideoxy-3'-thiacytidine 5'-phosphate | - |
? | |
2.7.1.74 | NTP + troxacitabine | activation of the clinically relevant substrate | Homo sapiens | ? | - |
? | |
2.7.1.74 | UTP + (-)-beta-2',3'-dideoxy-3'-thiacytidine | - |
Homo sapiens | UDP + (-)-beta-2',3'-dideoxy-3'-thiacytidine 5'-phosphate | - |
? | |
2.7.1.74 | UTP + D-2'-deoxycytidine | - |
Homo sapiens | UDP + D-2'-deoxycytidine 5'-phosphate | - |
? | |
2.7.1.74 | UTP + L-2'-deoxycytidine | - |
Homo sapiens | UDP + L-2'-deoxycytidine 5'-phosphate | - |
? | |
2.7.1.74 | UTP + troxacitabine | - |
Homo sapiens | UDP + troxacitabine 5'-phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.74 | dCK | - |
Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.74 | 0.01 | - |
UTP | cosubstrate L-2'-deoxycytidine, wild-type, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.012 | - |
ATP | cosubstrate L-2'-deoxycytidine, wild-type, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.028 | - |
(-)-beta-2',3'-dideoxy-3'-thiacytidine | C9S/C45S/C59S/C146S mutant enzyme with ATP as phosphate donor, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.028 | - |
ATP | cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.03 | - |
(-)-beta-2',3'-dideoxy-3'-thiacytidine | wild type enzyme with ATP as phosphate donor, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.03 | - |
ATP | cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, wild-type, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.033 | - |
ATP | cosubstrate D-2'-deoxycytidine, wild-type, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.036 | - |
ATP | cosubstrate L-2'-deoxycytidine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.042 | - |
UTP | cosubstrate L-2'-deoxycytidine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.049 | - |
UTP | cosubstrate D-2'-deoxycytidine, wild-type, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.072 | - |
(-)-beta-2',3'-dideoxy-3'-thiacytidine | C9S/C45S/C59S/C146S mutant enzyme with UTP as phosphate donor, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.072 | - |
UTP | cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.095 | - |
troxacitabine | wild type enzyme with ATP as phosphate donor, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.095 | - |
ATP | cosubstrate troxacitabine, wild-type, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.102 | - |
(-)-beta-2',3'-dideoxy-3'-thiacytidine | wild type enzyme with UTP as phosphate donor, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.102 | - |
UTP | cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, wild-type, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.173 | - |
ATP | cosubstrate D-2'-deoxycytidine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.179 | - |
troxacitabine | wild type enzyme with UTP as phosphate donor, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.179 | - |
UTP | cosubstrate troxacitabine, wild-type, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.192 | - |
troxacitabine | C9S/C45S/C59S/C146S mutant enzyme with ATP as phosphate donor, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.192 | - |
ATP | cosubstrate troxacitabine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.217 | - |
UTP | cosubstrate D-2'-deoxycytidine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.524 | - |
troxacitabine | C9S/C45S/C59S/C146S mutant enzyme with UTP as phosphate donor, 37°C, pH 7.5 | Homo sapiens | |
2.7.1.74 | 0.524 | - |
UTP | cosubstrate troxacitabine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5 | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.74 | ATP | - |
Homo sapiens | |
2.7.1.74 | UTP | - |
Homo sapiens |