BRENDA - Enzyme Database show

Structural basis for activation of the therapeutic L-nucleoside analogs 3TC and troxacitabine by human deoxycytidine kinase

Sabini, E.; Hazra, S.; Konrad, M.; Burley, S.K.; Lavie, A.; Nucleic Acids Res. 35, 186-192 (2007)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.74
mutant protein expressed in Escherichia coli
Homo sapiens
Crystallization (Commentary)
EC Number
Crystallization
Organism
2.7.1.74
hanging drop vapour diffusion method, mutant enzyme crystallized in complex with its substrates (-)-beta-2’,3’-dideoxy-3’-thiacytidine and troxacitabine; mutant C9S/C45S/C59S/C146S in complex with L-nucleosides (-)-beta-2',3'-dideoxy-3'-thiacytidine and troxacitabine. The nucleoside binding-site tolerates substrates with different chiral configurations by maintaining virtually all of the protein-ligand interactions responsible for productive substrate positioning. The pseudo-symmetry of nucleosides and nucleoside analogs in combination with their conformational flexibility allows the L- and D-enantiomeric forms to adopt similar shapes when bound to the enzyme
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.7.1.74
C9S/C45S/C59S/C146S
engineered protein has a significantly improved crystallization behaviour compared with the wild type protein; mutation of the four surface-exposed Cys residues, constructued for crystallization. Mutant displays moderate kinetic differences in the catalytic efficiency when compared with wild-type
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.003
-
(-)-beta-2',3'-dideoxy-3'-thiacytidine
wild type enzyme with ATP as phosphate donor, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.0034
-
ATP
cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, wild-type, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.008
-
(-)-beta-2',3'-dideoxy-3'-thiacytidine
C9S/C45S/C59S/C146S mutant enzyme with ATP as phosphate donor, 37°C, pH 7.5; wild type enzyme with UTP as phosphate donor, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.008
-
UTP
cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, wild-type, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.0084
-
ATP
cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.0106
-
UTP
cosubstrate troxacitabine, wild-type, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.011
-
troxacitabine
wild type enzyme with UTP as phosphate donor, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.013
-
troxacitabine
wild type enzyme with ATP as phosphate donor, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.0132
-
ATP
cosubstrate troxacitabine, wild-type, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.0149
-
UTP
cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.015
-
(-)-beta-2',3'-dideoxy-3'-thiacytidine
C9S/C45S/C59S/C146S mutant enzyme with UTP as phosphate donor, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.0568
-
ATP
cosubstrate troxacitabine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.057
-
troxacitabine
C9S/C45S/C59S/C146S mutant enzyme with ATP as phosphate donor, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.0578
-
UTP
cosubstrate troxacitabine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.058
-
troxacitabine
C9S/C45S/C59S/C146Smutant enzyme with UTP as phosphate donor, 37°C, pH 7.5
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
in complex with ATP or UTP
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.74
additional information
Homo sapiens
dCK is able to phosphorylate both D- and L-nucleosides and nucleoside analogs
?
-
-
-
2.7.1.74
NTP + (-)-beta-2',3'-dideoxy-3'-thiacytidine
Homo sapiens
activation of the clinically relevant substrate
NDP + (-)-beta-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
-
-
?
2.7.1.74
NTP + troxacitabine
Homo sapiens
activation of the clinically relevant substrate
?
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Homo sapiens
-
-
-
2.7.1.74
Homo sapiens
P27707
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.74
recombinant protein from Escherichia coli
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + (-)-beta-2',3'-dideoxy-3'-thiacytidine
-
676221
Homo sapiens
ADP + (-)-beta-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + (-)-beta-2',3'-dideoxy-3'-thiacytidine
i.e. lamivudine
676221
Homo sapiens
ADP + (-)-beta-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + D-2'-deoxycytidine
-
676221
Homo sapiens
ADP + D-2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + L-2'-deoxycytidine
-
676221
Homo sapiens
ADP + L-2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + troxacitabine
-
676221
Homo sapiens
ADP + troxacitabine 5'-phosphate
-
-
-
?
2.7.1.74
additional information
dCK is able to phosphorylate both D- and L-nucleosides and nucleoside analogs
676221
Homo sapiens
?
-
-
-
-
2.7.1.74
NTP + (-)-beta-2',3'-dideoxy-3'-thiacytidine
activation of the clinically relevant substrate
676221
Homo sapiens
NDP + (-)-beta-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
-
-
-
?
2.7.1.74
NTP + troxacitabine
activation of the clinically relevant substrate
676221
Homo sapiens
?
-
-
-
?
2.7.1.74
UTP + (-)-beta-2',3'-dideoxy-3'-thiacytidine
-
676221
Homo sapiens
UDP + (-)-beta-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + D-2'-deoxycytidine
-
676221
Homo sapiens
UDP + D-2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + L-2'-deoxycytidine
-
676221
Homo sapiens
UDP + L-2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + troxacitabine
-
676221
Homo sapiens
UDP + troxacitabine 5'-phosphate
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.01
-
UTP
cosubstrate L-2'-deoxycytidine, wild-type, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.012
-
ATP
cosubstrate L-2'-deoxycytidine, wild-type, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.028
-
ATP
cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.028
-
(-)-beta-2',3'-dideoxy-3'-thiacytidine
C9S/C45S/C59S/C146S mutant enzyme with ATP as phosphate donor, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.03
-
ATP
cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, wild-type, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.03
-
(-)-beta-2',3'-dideoxy-3'-thiacytidine
wild type enzyme with ATP as phosphate donor, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.033
-
ATP
cosubstrate D-2'-deoxycytidine, wild-type, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.036
-
ATP
cosubstrate L-2'-deoxycytidine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.042
-
UTP
cosubstrate L-2'-deoxycytidine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.049
-
UTP
cosubstrate D-2'-deoxycytidine, wild-type, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.072
-
(-)-beta-2',3'-dideoxy-3'-thiacytidine
C9S/C45S/C59S/C146S mutant enzyme with UTP as phosphate donor, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.072
-
UTP
cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.095
-
ATP
cosubstrate troxacitabine, wild-type, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.095
-
troxacitabine
wild type enzyme with ATP as phosphate donor, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.102
-
(-)-beta-2',3'-dideoxy-3'-thiacytidine
wild type enzyme with UTP as phosphate donor, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.102
-
UTP
cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, wild-type, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.173
-
ATP
cosubstrate D-2'-deoxycytidine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.179
-
UTP
cosubstrate troxacitabine, wild-type, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.192
-
ATP
cosubstrate troxacitabine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.192
-
troxacitabine
C9S/C45S/C59S/C146S mutant enzyme with ATP as phosphate donor, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.217
-
UTP
cosubstrate D-2'-deoxycytidine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.524
-
UTP
cosubstrate troxacitabine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
Homo sapiens
2.7.1.74
6.08
-
troxacitabine
C9S/C45S/C59S/C146S mutant enzyme with UTP as phosphate donor, 37°C, pH 7.5
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.74
ATP
-
Homo sapiens
2.7.1.74
UTP
-
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.74
mutant protein expressed in Escherichia coli
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.74
ATP
-
Homo sapiens
2.7.1.74
UTP
-
Homo sapiens
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
2.7.1.74
hanging drop vapour diffusion method, mutant enzyme crystallized in complex with its substrates (-)-beta-2’,3’-dideoxy-3’-thiacytidine and troxacitabine
Homo sapiens
2.7.1.74
mutant C9S/C45S/C59S/C146S in complex with L-nucleosides (-)-beta-2',3'-dideoxy-3'-thiacytidine and troxacitabine. The nucleoside binding-site tolerates substrates with different chiral configurations by maintaining virtually all of the protein-ligand interactions responsible for productive substrate positioning. The pseudo-symmetry of nucleosides and nucleoside analogs in combination with their conformational flexibility allows the L- and D-enantiomeric forms to adopt similar shapes when bound to the enzyme
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.7.1.74
C9S/C45S/C59S/C146S
engineered protein has a significantly improved crystallization behaviour compared with the wild type protein
Homo sapiens
2.7.1.74
C9S/C45S/C59S/C146S
mutation of the four surface-exposed Cys residues, constructued for crystallization. Mutant displays moderate kinetic differences in the catalytic efficiency when compared with wild-type
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.003
-
(-)-beta-2',3'-dideoxy-3'-thiacytidine
wild type enzyme with ATP as phosphate donor, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.0034
-
ATP
cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, wild-type, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.008
-
(-)-beta-2',3'-dideoxy-3'-thiacytidine
C9S/C45S/C59S/C146S mutant enzyme with ATP as phosphate donor, 37°C, pH 7.5; wild type enzyme with UTP as phosphate donor, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.008
-
UTP
cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, wild-type, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.0084
-
ATP
cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.0106
-
UTP
cosubstrate troxacitabine, wild-type, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.011
-
troxacitabine
wild type enzyme with UTP as phosphate donor, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.013
-
troxacitabine
wild type enzyme with ATP as phosphate donor, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.0132
-
ATP
cosubstrate troxacitabine, wild-type, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.0149
-
UTP
cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.015
-
(-)-beta-2',3'-dideoxy-3'-thiacytidine
C9S/C45S/C59S/C146S mutant enzyme with UTP as phosphate donor, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.0568
-
ATP
cosubstrate troxacitabine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.057
-
troxacitabine
C9S/C45S/C59S/C146S mutant enzyme with ATP as phosphate donor, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.0578
-
UTP
cosubstrate troxacitabine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.058
-
troxacitabine
C9S/C45S/C59S/C146Smutant enzyme with UTP as phosphate donor, 37°C, pH 7.5
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
in complex with ATP or UTP
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.74
additional information
Homo sapiens
dCK is able to phosphorylate both D- and L-nucleosides and nucleoside analogs
?
-
-
-
2.7.1.74
NTP + (-)-beta-2',3'-dideoxy-3'-thiacytidine
Homo sapiens
activation of the clinically relevant substrate
NDP + (-)-beta-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
-
-
?
2.7.1.74
NTP + troxacitabine
Homo sapiens
activation of the clinically relevant substrate
?
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.74
recombinant protein from Escherichia coli
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + (-)-beta-2',3'-dideoxy-3'-thiacytidine
-
676221
Homo sapiens
ADP + (-)-beta-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + (-)-beta-2',3'-dideoxy-3'-thiacytidine
i.e. lamivudine
676221
Homo sapiens
ADP + (-)-beta-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + D-2'-deoxycytidine
-
676221
Homo sapiens
ADP + D-2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + L-2'-deoxycytidine
-
676221
Homo sapiens
ADP + L-2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + troxacitabine
-
676221
Homo sapiens
ADP + troxacitabine 5'-phosphate
-
-
-
?
2.7.1.74
additional information
dCK is able to phosphorylate both D- and L-nucleosides and nucleoside analogs
676221
Homo sapiens
?
-
-
-
-
2.7.1.74
NTP + (-)-beta-2',3'-dideoxy-3'-thiacytidine
activation of the clinically relevant substrate
676221
Homo sapiens
NDP + (-)-beta-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
-
-
-
?
2.7.1.74
NTP + troxacitabine
activation of the clinically relevant substrate
676221
Homo sapiens
?
-
-
-
?
2.7.1.74
UTP + (-)-beta-2',3'-dideoxy-3'-thiacytidine
-
676221
Homo sapiens
UDP + (-)-beta-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + D-2'-deoxycytidine
-
676221
Homo sapiens
UDP + D-2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + L-2'-deoxycytidine
-
676221
Homo sapiens
UDP + L-2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
UTP + troxacitabine
-
676221
Homo sapiens
UDP + troxacitabine 5'-phosphate
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.01
-
UTP
cosubstrate L-2'-deoxycytidine, wild-type, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.012
-
ATP
cosubstrate L-2'-deoxycytidine, wild-type, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.028
-
ATP
cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.028
-
(-)-beta-2',3'-dideoxy-3'-thiacytidine
C9S/C45S/C59S/C146S mutant enzyme with ATP as phosphate donor, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.03
-
ATP
cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, wild-type, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.03
-
(-)-beta-2',3'-dideoxy-3'-thiacytidine
wild type enzyme with ATP as phosphate donor, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.033
-
ATP
cosubstrate D-2'-deoxycytidine, wild-type, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.036
-
ATP
cosubstrate L-2'-deoxycytidine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.042
-
UTP
cosubstrate L-2'-deoxycytidine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.049
-
UTP
cosubstrate D-2'-deoxycytidine, wild-type, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.072
-
(-)-beta-2',3'-dideoxy-3'-thiacytidine
C9S/C45S/C59S/C146S mutant enzyme with UTP as phosphate donor, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.072
-
UTP
cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.095
-
ATP
cosubstrate troxacitabine, wild-type, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.095
-
troxacitabine
wild type enzyme with ATP as phosphate donor, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.102
-
(-)-beta-2',3'-dideoxy-3'-thiacytidine
wild type enzyme with UTP as phosphate donor, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.102
-
UTP
cosubstrate (-)-beta-2',3'-dideoxy-3'-thiacytidine, wild-type, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.173
-
ATP
cosubstrate D-2'-deoxycytidine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.179
-
UTP
cosubstrate troxacitabine, wild-type, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.192
-
ATP
cosubstrate troxacitabine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.192
-
troxacitabine
C9S/C45S/C59S/C146S mutant enzyme with ATP as phosphate donor, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.217
-
UTP
cosubstrate D-2'-deoxycytidine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
Homo sapiens
2.7.1.74
0.524
-
UTP
cosubstrate troxacitabine, mutant C9S/C45S/C59S/C146S, 37°C, pH 7.5
Homo sapiens
2.7.1.74
6.08
-
troxacitabine
C9S/C45S/C59S/C146S mutant enzyme with UTP as phosphate donor, 37°C, pH 7.5
Homo sapiens