EC Number | Cloned (Comment) | Organism |
---|---|---|
5.1.1.3 | Escherichia coli strains DH5a and BL26(DE3) are used for cloning and overexpression of mycobacterial MurI, respectively | Mycobacterium tuberculosis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.1.1.3 | 28800 | - |
MALDI-TOF analysis | Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.1.1.3 | Mycobacterium tuberculosis | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
5.1.1.3 | additional information | - |
it is demonstrated that mycobacterial MurI inhibits DNA gyrase activity. Inhibition is not species-specific as Escherichia coli gyrase is also inhibited but is enzyme-specific as topoisomerase I activity remains unaltered. MurI binds to GyrA subunit of the enzyme leading to a decrease in DNA-binding of the holoenzyme. The sequestration of the gyrase by MurI results in inhibition of all reactions catalysed by DNA gyrase. MurI is thus not a typical potent inhibitor of DNA gyrase and instead its role could be in modulation of the gyrase activity | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.1.1.3 | L-glutamate | - |
Mycobacterium tuberculosis | D-glutamate | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.1.1.3 | glutamate racemase | - |
Mycobacterium tuberculosis |
5.1.1.3 | MurI | - |
Mycobacterium tuberculosis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.1.1.3 | 37 | - |
assay at | Mycobacterium tuberculosis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.1.1.3 | 8 | - |
assay at | Mycobacterium tuberculosis |