Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sureka, K.; Dey, S.; Datta, P.; Singh, A.K.; Dasgupta, A.; Rodrigue, S.; Basu, J.; Kundu, M.
    Polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria (2007), Mol. Microbiol., 65, 261-276.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.4.1 medicine PPK1 is necessary for survival under anaerobic conditions or oxidative stress Mycolicibacterium smegmatis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.1 gene ppk1, expression of wild-type and mutant enzymes Mycolicibacterium smegmatis
2.7.4.1 gene ppk1, expression of wild-type and mutant enzymes Mycobacterium tuberculosis
2.7.4.1 overexpression in Escherichia coli Mycolicibacterium smegmatis

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.1 D395A site-directed mutagenesis, slightly reduced forward and reverse reaction activities compared to the wild-type enzyme Mycolicibacterium smegmatis
2.7.4.1 F176A site-directed mutagenesis, highly reduced forward and reverse reaction activities compared to the wild-type enzyme Mycolicibacterium smegmatis
2.7.4.1 F176Y site-directed mutagenesis, slightly reduced forward and reverse reaction activities compared to the wild-type enzyme Mycolicibacterium smegmatis
2.7.4.1 G347A site-directed mutagenesis, slightly reduced forward and reverse reaction activities compared to the wild-type enzyme Mycolicibacterium smegmatis
2.7.4.1 H491A site-directed mutagenesis, reduced forward and reverse reaction activities compared to the wild-type enzyme Mycolicibacterium smegmatis
2.7.4.1 H491A/H510A site-directed mutagenesis, highly reduced forward and reverse reaction activities compared to the wild-type enzyme Mycolicibacterium smegmatis
2.7.4.1 H510A site-directed mutagenesis, reduced forward and reverse reaction activities compared to the wild-type enzyme Mycolicibacterium smegmatis
2.7.4.1 L234A site-directed mutagenesis, slightly reduced forward and reverse reaction activities compared to the wild-type enzyme Mycolicibacterium smegmatis
2.7.4.1 additional information PPK knockout organism is susceptible to oxidative stress Mycolicibacterium smegmatis
2.7.4.1 additional information downregulation of ppk1 leads to impaired survival of Mycobacterium tuberculosis in macrophages, the stringent response regulator ppGpp is impaired in the ppk1 knockout mutant and is restored after complementation with ppk1 of Mycobacterium tuberculosis, overview Mycobacterium tuberculosis
2.7.4.1 additional information effect of oxidative and surface stress as well as anaerobiosis on the survival of a ppk1 knockout mutant PPK-KO, overview. Effect of inactivation of ppk1 on the transcription of mprA, mprB, sigE and rel, overview Mycolicibacterium smegmatis
2.7.4.1 P225A site-directed mutagenesis, slightly reduced forward and reverse reaction activities compared to the wild-type enzyme Mycolicibacterium smegmatis
2.7.4.1 R230A site-directed mutagenesis, highly reduced forward and reverse reaction activities compared to the wild-type enzyme Mycolicibacterium smegmatis
2.7.4.1 R230K site-directed mutagenesis, slightly reduced forward and reverse reaction activities compared to the wild-type enzyme Mycolicibacterium smegmatis
2.7.4.1 R309A site-directed mutagenesis, reduced forward and reverse reaction activities compared to the wild-type enzyme Mycolicibacterium smegmatis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.1 membrane
-
 Mycolicibacterium smegmatis 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.1 Mg2+
-
 Mycolicibacterium smegmatis
2.7.4.1 Mg2+
-
 Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.1 81000
-
 Western blot Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.1 ATP + (phosphate)n Mycolicibacterium smegmatis
-
 ADP + (phosphate)n+1
-
 r
2.7.4.1 ATP + (phosphate)n Mycobacterium tuberculosis
-
 ADP + (phosphate)n+1
-
 r
2.7.4.1 ATP + (phosphate)n Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
-
 ADP + (phosphate)n+1
-
 r
2.7.4.1 ATP + (phosphate)n Mycobacterium tuberculosis H37Rv
-
 ADP + (phosphate)n+1
-
 r
2.7.4.1 ATP + (phosphate)n Mycolicibacterium smegmatis mc(2)155
-
 ADP + (phosphate)n+1
-
 r
2.7.4.1 additional information Mycolicibacterium smegmatis polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria, mprAB-sigE-rel signalling cascade, overview. PPK1 is required for the stringent response, under conditions of stress polyP acts as a preferred donor for MprB-mediated phosphorylation of MprA facilitating transcription of the sigE gene thereby leading finally to the enhancement of the transcription of rel in Mycobacterium smegmatis, overview ?
-
 ?
2.7.4.1 additional information Mycobacterium tuberculosis polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria, PPK1 is required for the stringent response, under conditions of stress polyP acts as a preferred donor for MprB-mediated phosphorylation of MprA facilitating transcription of the sigE gene thereby leading finally to the enhancement of the transcription of rel in Mycobacterium tuberculosis, overview ?
-
 ?
2.7.4.1 additional information Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria, mprAB-sigE-rel signalling cascade, overview. PPK1 is required for the stringent response, under conditions of stress polyP acts as a preferred donor for MprB-mediated phosphorylation of MprA facilitating transcription of the sigE gene thereby leading finally to the enhancement of the transcription of rel in Mycobacterium smegmatis, overview ?
-
 ?
2.7.4.1 additional information Mycobacterium tuberculosis H37Rv polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria, PPK1 is required for the stringent response, under conditions of stress polyP acts as a preferred donor for MprB-mediated phosphorylation of MprA facilitating transcription of the sigE gene thereby leading finally to the enhancement of the transcription of rel in Mycobacterium tuberculosis, overview ?
-
 ?
2.7.4.1 additional information Mycolicibacterium smegmatis mc(2)155 polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria, mprAB-sigE-rel signalling cascade, overview. PPK1 is required for the stringent response, under conditions of stress polyP acts as a preferred donor for MprB-mediated phosphorylation of MprA facilitating transcription of the sigE gene thereby leading finally to the enhancement of the transcription of rel in Mycobacterium smegmatis, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.1 Mycobacterium tuberculosis P9WHV9 gene ppk
-
2.7.4.1 Mycobacterium tuberculosis H37Rv P9WHV9 gene ppk
-
2.7.4.1 Mycolicibacterium smegmatis
-
-
-
2.7.4.1 Mycolicibacterium smegmatis
-
 gene ppk1
-
2.7.4.1 Mycolicibacterium smegmatis mc(2)155
-
-
-
2.7.4.1 Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
-
 gene ppk1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.1 of the recombinant His x6-tagged protein Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.1 additional information
-
 relative activity of mutant enzymes compared to the wild-type in both reaction directions, overview Mycolicibacterium smegmatis
2.7.4.1 0.7
-
 synthesis of ATP, reversed reaction Mycolicibacterium smegmatis
2.7.4.1 4.2
-
 of the recombinant protein Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.1 ATP + (phosphate)n
-
 Mycolicibacterium smegmatis ADP + (phosphate)n+1
-
 r
2.7.4.1 ATP + (phosphate)n
-
 Mycobacterium tuberculosis ADP + (phosphate)n+1
-
 r
2.7.4.1 ATP + (phosphate)n
-
 Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 ADP + (phosphate)n+1
-
 r
2.7.4.1 ATP + (phosphate)n
-
 Mycobacterium tuberculosis H37Rv ADP + (phosphate)n+1
-
 r
2.7.4.1 ATP + (phosphate)n
-
 Mycolicibacterium smegmatis mc(2)155 ADP + (phosphate)n+1
-
 r
2.7.4.1 additional information polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria, mprAB-sigE-rel signalling cascade, overview. PPK1 is required for the stringent response, under conditions of stress polyP acts as a preferred donor for MprB-mediated phosphorylation of MprA facilitating transcription of the sigE gene thereby leading finally to the enhancement of the transcription of rel in Mycobacterium smegmatis, overview Mycolicibacterium smegmatis ?
-
 ?
2.7.4.1 additional information polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria, PPK1 is required for the stringent response, under conditions of stress polyP acts as a preferred donor for MprB-mediated phosphorylation of MprA facilitating transcription of the sigE gene thereby leading finally to the enhancement of the transcription of rel in Mycobacterium tuberculosis, overview Mycobacterium tuberculosis ?
-
 ?
2.7.4.1 additional information polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria, mprAB-sigE-rel signalling cascade, overview. PPK1 is required for the stringent response, under conditions of stress polyP acts as a preferred donor for MprB-mediated phosphorylation of MprA facilitating transcription of the sigE gene thereby leading finally to the enhancement of the transcription of rel in Mycobacterium smegmatis, overview Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 ?
-
 ?
2.7.4.1 additional information polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria, PPK1 is required for the stringent response, under conditions of stress polyP acts as a preferred donor for MprB-mediated phosphorylation of MprA facilitating transcription of the sigE gene thereby leading finally to the enhancement of the transcription of rel in Mycobacterium tuberculosis, overview Mycobacterium tuberculosis H37Rv ?
-
 ?
2.7.4.1 additional information polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria, mprAB-sigE-rel signalling cascade, overview. PPK1 is required for the stringent response, under conditions of stress polyP acts as a preferred donor for MprB-mediated phosphorylation of MprA facilitating transcription of the sigE gene thereby leading finally to the enhancement of the transcription of rel in Mycobacterium smegmatis, overview Mycolicibacterium smegmatis mc(2)155 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.1 dimer
-
 Mycolicibacterium smegmatis

Synonyms

EC Number Synonyms Comment Organism
2.7.4.1 ATP:polyphosphate phosphotransferase
-
 Mycolicibacterium smegmatis
2.7.4.1 kinase, polyphosphate (phosphorylating)
-
 Mycolicibacterium smegmatis
2.7.4.1 polyphosphate kinase
-
 Mycolicibacterium smegmatis
2.7.4.1 polyphosphate kinase 1
-
 Mycolicibacterium smegmatis
2.7.4.1 polyphosphate kinase 1
-
 Mycobacterium tuberculosis
2.7.4.1 PPK1
-
 Mycolicibacterium smegmatis
2.7.4.1 PPK1
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.4.1 ATP
-
 Mycolicibacterium smegmatis
2.7.4.1 ATP
-
 Mycobacterium tuberculosis