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Literature summary extracted from

  • Mohammadi, T.; Karczmarek, A.; Crouvoisier, M.; Bouhss, A.; Mengin-Lecreulx, D.; den Blaauwen, T.
    The essential peptidoglycan glycosyltransferase MurG forms a complex with proteins involved in lateral envelope growth as well as with proteins involved in cell division in Escherichia coli (2007), Mol. Microbiol., 65, 1106-1121.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.4.1.227 biotechnology MurG is interesting to evaluate as a potential antibiotic target, as it has no counterpart in mammalian cells Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.227 the coding sequence is amplified from the Escherichia coli K12 chromosome and cloned for expression of His-tagged MurG in JM109 cells Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.227 cell envelope
-
 Escherichia coli 30313
-
2.4.1.227 inner membrane
-
 Escherichia coli
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.227 37800
-
 determined by SDS-PAGE and Western Blot analysis Escherichia coli
2.7.8.13 40000
-
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.227 UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol Escherichia coli
-
 UDP + GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 ?
2.4.1.227 UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol Escherichia coli LMC500
-
 UDP + GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.227 Escherichia coli
-
-
-
2.7.8.13 Escherichia coli
-
-
-
2.7.8.13 Escherichia coli LMC500
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.227 native MurG is extracted from inner membrane vesicles of Escherichia coli cells, His-tagged MurG is purified by Ni-affinity chromatography using His-bind resin Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.227 UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 Escherichia coli UDP + GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 ?
2.4.1.227 UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 Escherichia coli LMC500 UDP + GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 ?
2.7.8.13 UDP-NAc-muramoyl-pentapeptide + undecaprenyl phosphate
-
 Escherichia coli UMP + undecaprenyl-phospho-NAc-muramoyl-pentapeptide
-
 ?
2.7.8.13 UDP-NAc-muramoyl-pentapeptide + undecaprenyl phosphate
-
 Escherichia coli LMC500 UMP + undecaprenyl-phospho-NAc-muramoyl-pentapeptide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.227 MurG
-
 Escherichia coli
2.4.1.227 peptidoglycan glycosyltransferase
-
 Escherichia coli
2.7.8.13 MraY MurG, MreB and MraY are cross-linked in the same protein complex Escherichia coli