EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.198 | expressed in Escherichia coli DH5alpha | Aspergillus fumigatus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.1.198 | additional information | afpig-a null mutant, strain CEA17 (pyrG-). This mutant shows a complete loss in glycosylphosphatidylinositol-N-acetylglucoaminyltransferase activity. None of the membrane bound glycosylphosphatidylinositol-anchored phospholipases, phosphatase, eCM33por Gl1p is found in the membrane preparation of the mutant. The mutant can grow at temperatures from 30°C to 50°C, but the growth rates are greatly inhibited. When the mutant is grown in the presence of various cell wall-disrupting agents, it is more sensitive to Calcofluor white, Congo-red, Nikkomycin Z and SDS, than the wild type. The mannoproteins and beta-glucans in mycelial cell wall of the mutant is 2.5fold and 2fold of the wild type or revertant respectively. The deletion of the afpig-a gene leads to earlier polarity, germ tube emergence and septation of mutant conidiospores in the early duplication cycles and to significant changes in developmental events and morphogenesis during conidiation. Two virulence factors, mycelial catalase Cat1 and Asp-haemolysin, are missing in the mutant, but the chitinase ChiB is found remarkably increased. After the inoculation of wild-type and mutant conidia into immunocompromised mice, early mortality is nearly identical among all groups. The remaining mice receiving the deletion mutant survived significantly longer. | Aspergillus fumigatus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.198 | Aspergillus fumigatus | Q6Q2B4 | afpig-a; Most common opportunistic fungal pathogen of human | - |
2.4.1.198 | Aspergillus fumigatus YJ-407 | Q6Q2B4 | afpig-a; Most common opportunistic fungal pathogen of human | - |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.198 | afpig-a | - |
Aspergillus fumigatus |
2.4.1.198 | afpig-a | gene is the homologue of GPI3 /SPT14 /pig-A gene (encode the catalytic subunit of glycosylphosphatidylinositol-N-acetylglucoaminyltransferase complex) in Aspergillus fumigatus | Aspergillus fumigatus |
2.4.1.198 | GPI-GnT | - |
Aspergillus fumigatus |
2.4.1.198 | GPI-N-acetylglucosaminyltransferase complex | - |
Aspergillus fumigatus |