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Literature summary extracted from

  • Dawson, A.; Gibellini, F.; Sienkiewicz, N.; Tulloch, L.B.; Fyfe, P.K.; McLuskey, K.; Fairlamb, A.H.; Hunter, W.N.
    Structure and reactivity of Trypanosoma brucei pteridine reductase: inhibition by the archetypal antifolate methotrexate (2006), Mol. Microbiol., 61, 1457-1468.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.33
-
 Trypanosoma brucei

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.5.1.33
-
 Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.33 dihydrobiopterin substrate inhibition Trypanosoma brucei
1.5.1.33 methotrexate
-
 Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.33 0.0109
-
 dihydrobiopterin pH 3.7 Trypanosoma brucei

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.33 Trypanosoma brucei
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.33 0.1
-
 pH 3.7, substrate dihydrofolate Trypanosoma brucei
1.5.1.33 0.1
-
 pH 3.7, substrate folate Trypanosoma brucei
1.5.1.33 2
-
 pH 3.7, substrate biopterin Trypanosoma brucei
1.5.1.33 2.3
-
 pH 3.7, substrate dihydrobiopterin Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.33 Biopterin + NADPH
-
 Trypanosoma brucei 5,6,7,8-Tetrahydrobiopterin + NADP+
-
 ?
1.5.1.33 dihydrobiopterin + NADPH
-
 Trypanosoma brucei 5,6,7,8-tetrahydrobiopterin + NADP+
-
 ?
1.5.1.33 Dihydrofolate + NADPH
-
 Trypanosoma brucei 5,6,7,8-Tetrahydrofolate + NADP+
-
 ?
1.5.1.33 folate + NADPH
-
 Trypanosoma brucei 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.33 tetramer crystallization data Trypanosoma brucei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.33 4.7
-
 dihydrobiopterin pH 3.7 Trypanosoma brucei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.33 3.7
-
-
 Trypanosoma brucei

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.33 NADPH
-
 Trypanosoma brucei

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.33 0.000152
-
 methotrexate pH 3.7 Trypanosoma brucei
1.5.1.33 0.0038
-
 dihydrobiopterin pH 3.7 Trypanosoma brucei