Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Koga, K.; Harada, T.; Shimizu, H.; Tanaka, K.
    Bacterial luciferase activity and the intracellular redox pool in Escherichia coli (2005), Mol. Genet. Genomics, 274, 180-188.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.8 expressed in Escherichia coli BL21(DE3)(pLysS) cells Aliivibrio fischeri
1.14.14.3 genes luxA and luxB, expression under the control of a consensus-type promoter, lacUV5, in Escherichia coli, activity declines abruptly upon entry into the stationary growth phase, while the levels of luciferase proteins remian constant, the phenomenon, termed ADLA, i.e. abrupt decline of luciferase activity, is caused by a decrease in the availability of flavin mononucleotide Aliivibrio fischeri

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.3 beetle luciferin + FMNH2 + O2 Aliivibrio fischeri
-
 ?
-
 ir
1.14.14.3 n-caprinaldehyde + FMNH2 + O2 Aliivibrio fischeri
-
 n-caprinoate + FMN + H2O + hv
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.8 Aliivibrio fischeri
-
-
-
1.14.14.3 Aliivibrio fischeri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.8 Ni-NTA agarose chromatography Aliivibrio fischeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.8 long-chain aldehyde + FMNH2 + O2
-
 Aliivibrio fischeri long-chain fatty acid + FMN + H2O + hv
-
 ?
1.14.14.3 beetle luciferin + FMNH2 + O2
-
 Aliivibrio fischeri ?
-
 ir
1.14.14.3 n-caprinaldehyde + FMNH2 + O2
-
 Aliivibrio fischeri n-caprinoate + FMN + H2O + hv
-
 ir

Synonyms

EC Number Synonyms Comment Organism
1.13.12.8 bacterial luciferase
-
 Aliivibrio fischeri
1.13.12.8 LuxAB
-
 Aliivibrio fischeri
1.14.14.3 bacterial luciferase
-
 Aliivibrio fischeri
1.14.14.3 LuxA
-
 Aliivibrio fischeri
1.14.14.3 LuxB
-
 Aliivibrio fischeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.3 7
-
 assay at Aliivibrio fischeri

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.3 FMNH2
-
 Aliivibrio fischeri