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Literature summary extracted from

  • Shen, Y.; Chou, C.Y.; Chang, G.G.; Tong, L.
    Is dimerization required for the catalytic activity of bacterial biotin carboxylase? (2006), Mol. Cell, 22, 807-818.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.4.14 sitting-drop vapor diffusion method, crystallization of mutant enzyme E23R and F363A Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.3.4.14 E23R mutant enzyme is monomeric in solution, mutant shows 3fold loss in catalytic activity, mutant enzyme forms the correct dimer at high concentrations. kcat/Km for ATP-hydrolysis is 2.6fold lower than wild-type value Escherichia coli
6.3.4.14 F363A mutant enzyme forms the correct dimer at high concentrations. kcat/Km for ATP-hydrolysis is identical to wild-type value Escherichia coli
6.3.4.14 R19E mutant enzyme is monomeric in solution, mutant shows 3fold loss in catalytic activity. kcat/Km for ATP-hydrolysis is 2.5fold lower than wild-type value Escherichia coli
6.3.4.14 R366E mutant enzyme shows no specific activity at 2.5 mM of enzyme and up to 800 mM of ATP Escherichia coli
6.3.4.14 R401E mutant enzyme shows no specific activity at 2.5 mM of enzyme and up to 800 mM of ATP Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.14 0.093
-
 ATP biotin-dependent ATP hydrolysis, mutant enzyme E23R Escherichia coli
6.3.4.14 0.0948
-
 ATP biotin-dependent ATP hydrolysis, mutant enzyme R19E Escherichia coli
6.3.4.14 0.1049
-
 ATP biotin-dependent ATP hydrolysis, mutant enzyme F363A Escherichia coli
6.3.4.14 0.1152
-
 ATP biotin-dependent ATP hydrolysis, wild-type enzyme Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.14 Escherichia coli P24182
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.14 ATP + biotin-carboxyl-carrier protein + CO2
-
 Escherichia coli ADP + phosphate + carboxybiotin-carboxyl-carrier protein
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.14 dimer wild-type enzyme, dimerization is not an absolute requirement for the catalytic activity of the Escherichia coli biotin carboxylase subunit Escherichia coli
6.3.4.14 monomer mutant enzyme R19E and E23R are monomeric in solution Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.4.14 0.071
-
 ATP biotin-dependent ATP hydrolysis, mutant enzyme E23R Escherichia coli
6.3.4.14 0.075
-
 ATP biotin-dependent ATP hydrolysis, mutant enzyme R19E Escherichia coli
6.3.4.14 0.207
-
 ATP biotin-dependent ATP hydrolysis, mutant enzyme F363A Escherichia coli
6.3.4.14 0.228
-
 ATP biotin-dependent ATP hydrolysis, wild-type enzyme Escherichia coli