Literature summary extracted from

  • Kurdrid, P.; Subudhi, S.; Hongsthong, A.; Ruengjitchatchawalya, M.; Tanticharoen, M.
    Functional expression of Spirulina-DELTA6 desaturase gene in yeast, Saccharomyces cerevisiae (2005), Mol. Biol. Rep., 32, 215-226.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.3 expressed in Saccharomyces cerevisiae, strain DBY746 with and without supplementation with linoleic acid Arthrospira platensis
1.14.19.46 expressed in Saccharomyces cerevisiae strain DBY746 Arthrospira platensis

Protein Variants

EC Number Protein Variants Comment Organism
1.14.19.3 H89G expressed in Saccharomyces cerevisiae, grown in SD medium in the presence of 50 microM of linoleic acid substrate, very low enzyme activity, can be rescued by 150 mM exogenous imidazole Arthrospira platensis
1.14.19.3 H129R expressed in Saccharomyces cerevisiae, grown in SD medium in the presence of 50 microM of linoleic acid substrate, no enzyme activity using linoleic acid as substrate Arthrospira platensis
1.14.19.3 H129G expressed in Saccharomyces cerevisiae, grown in SD medium in the presence of 50 microM of linoleic acid substrate, no enzyme activity, can be rescued by 150 mM exogenous imidazole Arthrospira platensis
1.14.19.3 H305G expressed in Saccharomyces cerevisiae, grown in SD medium in the presence of 50 microM of linoleic acid substrate, no enzyme activity, can be rescued by 150 mM exogenous imidazole Arthrospira platensis
1.14.19.3 H89R expressed in Saccharomyces cerevisiae, grown in SD medium in the presence of 50 microM of linoleic acid substrate, very low enzyme activity using linoleic acid as substrate Arthrospira platensis
1.14.19.3 H305R expressed in Saccharomyces cerevisiae, grown in SD medium in the presence of 50 microM of linoleic acid substrate, no enzyme activity using linoleic acid as substrate Arthrospira platensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.46 1-linoleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ Arthrospira platensis
-
1-gamma-linolenoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
?
1.14.19.46 1-linoleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ Arthrospira platensis C1
-
1-gamma-linolenoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.3 Arthrospira platensis
-
C1
-
1.14.19.46 Arthrospira platensis
-
-
-
1.14.19.3 Arthrospira platensis C1
-
C1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.

Synonyms

EC Number Synonyms Comment Organism

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.46 Ferredoxin
-
Arthrospira platensis