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Literature summary extracted from

  • Ali, V.; Nozaki, T.
    Biochemical and functional characterization of phosphoserine aminotransferase from Entamoeba histolytica, which possesses both phosphorylated and non-phosphorylated serine metabolic pathways (2006), Mol. Biochem. Parasitol., 145, 71-83.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.52 expression in Escherichia coli Arabidopsis thaliana
2.6.1.52 expression in Escherichia coli Entamoeba histolytica

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.52 O-acetylserine 10 mM, 50% inhibition Entamoeba histolytica
2.6.1.52 o-phospho-D-serine 10 mM, 70% inhibition Entamoeba histolytica
2.6.1.52 O-phospho-L-threonine 10 mM, 65% inhibition Entamoeba histolytica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.52 0.0368
-
 O-phospho-L-serine pH 8.5, 25°C Entamoeba histolytica
2.6.1.52 0.043
-
 2-oxoglutarate pH 8.5, 25°C Entamoeba histolytica
2.6.1.52 0.058
-
 3-phosphohydroxypyruvate pH 8.5, 25°C Entamoeba histolytica
2.6.1.52 0.0651
-
 2-oxoglutarate pH 8.5, 25°C Arabidopsis thaliana
2.6.1.52 0.225
-
 O-phospho-L-serine pH 8.5, 25°C Arabidopsis thaliana
2.6.1.52 0.788
-
 L-glutamate pH 8.5, 25°C Entamoeba histolytica
2.6.1.52 0.797
-
 3-phosphohydroxypyruvate pH 8.5, 25°C Arabidopsis thaliana
2.6.1.52 5.05
-
 L-glutamate pH 8.5, 25°C Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.52 Arabidopsis thaliana
-
-
-
2.6.1.52 Entamoeba histolytica Q60I38
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.52
-
 Arabidopsis thaliana
2.6.1.52
-
 Entamoeba histolytica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.52 0.47
-
 substrate: O-phospho-L-serine, pH 8.5, 25°C Arabidopsis thaliana
2.6.1.52 0.66
-
 substrate: 2-oxoglutarate, pH 8.5, 25°C Entamoeba histolytica
2.6.1.52 0.67
-
 substrate: O-phospho-L-serine, pH 8.5, 25°C Entamoeba histolytica
2.6.1.52 0.74
-
 substrate: 2-oxoglutarate, pH 8.5, 25°C Arabidopsis thaliana
2.6.1.52 5.82
-
 substrate: L-glutamate, pH 8.5, 25°C Entamoeba histolytica
2.6.1.52 6.14
-
 substrate: 3-phosphohydroxypyruvate, pH 8.5, 25°C Entamoeba histolytica
2.6.1.52 9.65
-
 substrate: 3-phosphohydroxypyruvate, pH 8.5, 25°C Arabidopsis thaliana
2.6.1.52 21.22
-
 substrate: L-glutamate, pH 8.5, 25°C Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.52 L-glutamate + 3-phosphohydroxypyruvate
-
 Arabidopsis thaliana O-phospho-L-serine + 2-oxoglutarate
-
 r
2.6.1.52 L-glutamate + 3-phosphohydroxypyruvate
-
 Entamoeba histolytica O-phospho-L-serine + 2-oxoglutarate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.6.1.52 AtPSAT
-
 Arabidopsis thaliana
2.6.1.52 EhPSAT
-
 Entamoeba histolytica
2.6.1.52 PSAT
-
 Arabidopsis thaliana
2.6.1.52 PSAT
-
 Entamoeba histolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.52 8 8.5 formation of 3-phosphohydroxypyruvate Entamoeba histolytica
2.6.1.52 8 9 formation of O-phospho-L-serine Entamoeba histolytica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.52 5.5 11 about 75% of maximal activit at pH 5.5 and pH 11.0, formation of 3-phosphohydroxypyruvate Entamoeba histolytica
2.6.1.52 7 10 about 50% of maximal activity at pH 7.0 and at pH 10.0, formation of O-phospho-L-serine Entamoeba histolytica

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.6.1.52 Entamoeba histolytica His-tagged recombinant enzyme
-
 8.43