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Literature summary extracted from

  • Cardoso, F.S.; Castro, R.F.; Borges, N.; Santos, H.
    Biochemical and genetic characterization of the pathways for trehalose metabolism in Propionibacterium freudenreichii, and their role in stress response (2007), Microbiology, 153, 270-280.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.15 heparin activates Propionibacterium freudenreichii
2.4.1.347 heparin activates Propionibacterium freudenreichii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.15 expression in Escherichia coli Propionibacterium freudenreichii
2.4.1.347 expression in Escherichia coli Propionibacterium freudenreichii
3.1.3.12 expressed in Escherichia coli strain JM107 Propionibacterium freudenreichii subsp. shermanii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.15 Cu2+
-
 Propionibacterium freudenreichii
2.4.1.15 NaCl an increase in the concentration of NaCl from 0 to 100 mM leads to a decrease in the OtsA activity by more than 35% when ADP-glucose or TDP-glucose is the substrate. When UDP-glucose or GDP-glucose is used as substrate, the OtsA activity is increased by 10–30% Propionibacterium freudenreichii
2.4.1.347 Cu2+
-
 Propionibacterium freudenreichii
2.4.1.347 NaCl an increase in the concentration of NaCl from 0 to 100 mM leads to a decrease in the OtsA activity by more than 35% when ADP-glucose or TDP-glucose is the substrate. When UDP-glucose or GDP-glucose is used as substrate, the OtsA activity is increased by 10-30% Propionibacterium freudenreichii
3.1.3.12 NaF strongly inhibited (70%) by 10 mM NaF Propionibacterium freudenreichii subsp. shermanii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.15 0.3
-
 UDP-glucose 37°C, pH 5.5 Propionibacterium freudenreichii
2.4.1.15 0.4
-
 GDP-glucose 37°C, pH 5.5 Propionibacterium freudenreichii
2.4.1.15 0.6
-
 ADP-glucose 37°C, pH 5.5 Propionibacterium freudenreichii
2.4.1.15 2
-
 TDP-glucose 37°C, pH 5.5 Propionibacterium freudenreichii
2.4.1.15 3
-
 D-glucose 6-phosphate 37°C, pH 5.5, cosubstrate: UDP-glucose Propionibacterium freudenreichii
2.4.1.15 4.2
-
 D-glucose 6-phosphate 37°C, pH 5.5, cosubstrate: ADP-glucose Propionibacterium freudenreichii
2.4.1.15 5.3
-
 D-glucose 6-phosphate 37°C, pH 5.5, cosubstrate: TDP-glucose Propionibacterium freudenreichii
2.4.1.15 7.1
-
 D-glucose 6-phosphate 37°C, pH 5.5, cosubstrate: GDP-glucose Propionibacterium freudenreichii
2.4.1.347 0.3
-
 UDP-glucose 37°C, pH 5.5 Propionibacterium freudenreichii
2.4.1.347 0.4
-
 GDP-glucose 37°C, pH 5.5 Propionibacterium freudenreichii
2.4.1.347 0.6
-
 ADP-glucose 37°C, pH 5.5 Propionibacterium freudenreichii
2.4.1.347 2
-
 TDP-glucose 37°C, pH 5.5 Propionibacterium freudenreichii
2.4.1.347 3
-
 D-glucose 6-phosphate 37°C, pH 5.5, cosubstrate: UDP-glucose Propionibacterium freudenreichii
2.4.1.347 4.2
-
 D-glucose 6-phosphate 37°C, pH 5.5, cosubstrate: ADP-glucose Propionibacterium freudenreichii
2.4.1.347 5.3
-
 D-glucose 6-phosphate 37°C, pH 5.5, cosubstrate: TDP-glucose Propionibacterium freudenreichii
2.4.1.347 7.1
-
 D-glucose 6-phosphate 37°C, pH 5.5, cosubstrate: GDP-glucose Propionibacterium freudenreichii
3.1.3.12 0.7
-
 trehalose 6-phosphate in MES buffer (50 mM, pH 6.5), 10 mM MgCl2, and 5 mM trehalose 6-phosphate, at 37°C Propionibacterium freudenreichii subsp. shermanii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.15 KCl upon an increase in the KCl concentration from 0 to 100 mM, the OtsA activity decreases by more than 40%, whereas it is not significantly affected when UDP or GDP-glucose is used as the substrate Propionibacterium freudenreichii
2.4.1.15 Mg2+ 10 mM, required for maximal activity Propionibacterium freudenreichii
2.4.1.15 NaCl an increase in the concentration of NaCl from 0 to 100 mM leads to a decrease in the OtsA activity by more than 35% when ADP-glucose or TDP-glucose is the substrate. When UDP-glucose or GDP-glucose is used as substrate, the OtsA activity is increased by 10–30% Propionibacterium freudenreichii
2.4.1.347 KCl upon an increase in the KCl concentration from 0 to 100 mM, the OtsA activity decreases by more than 40%, whereas it is not significantly affected when UDP or GDP-glucose is used as the substrate Propionibacterium freudenreichii
2.4.1.347 Mg2+ 10 mM, required for maximal activity Propionibacterium freudenreichii
2.4.1.347 NaCl an increase in the concentration of NaCl from 0 to 100 mM leads to a decrease in the OtsA activity by more than 35% when ADP-glucose or TDP-glucose is the substrate. When UDP-glucose or GDP-glucose is used as substrate, the OtsA activity is increased by 10-30% Propionibacterium freudenreichii
3.1.3.12 Mg2+ 10 mM, required for full activity Propionibacterium freudenreichii subsp. shermanii
3.1.3.12 additional information not activated by Co2+, Cu2+, Ca2+, and Mn2+ Propionibacterium freudenreichii subsp. shermanii
3.1.3.12 Na+ NaCl has a slightly negative effect on OtsB activity in the range of 0-250 mM, but is clearly inhibitory (over 30%) at higher concentrations Propionibacterium freudenreichii subsp. shermanii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.15 additional information Propionibacterium freudenreichii the trehalose-6-phosphate synthase/phosphatase (OtsA–OtsB) pathway plays an important role in the synthesis of trehalose in response to stress ?
-
 ?
2.4.1.347 additional information Propionibacterium freudenreichii the trehalose-6-phosphate synthase/phosphatase (OtsA-OtsB) pathway plays an important role in the synthesis of trehalose in response to stress ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.15 Propionibacterium freudenreichii
-
-
-
2.4.1.347 Propionibacterium freudenreichii
-
-
-
3.1.3.12 Propionibacterium freudenreichii subsp. shermanii
-
-
-
3.1.3.12 Propionibacterium freudenreichii subsp. shermanii NIZO B365
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.15
-
 Propionibacterium freudenreichii
2.4.1.347
-
 Propionibacterium freudenreichii
3.1.3.12 PD-10 column chromatography Propionibacterium freudenreichii subsp. shermanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.15 ADP-alpha-D-glucose + D-glucose 6-phosphate in crude extracts of Propionibacterium freudenreichii, OtsA is specific for ADP-glucose, in contrast to the pure recombinant OtsA, which uses UDP-glucose, GDP-glucose and TDP-glucose, in addition to ADP-glucose. The substrate specificity of OtsA in cell extracts is lost during purification, and the recombinant OtsA becomes specific to ADP-glucose upon incubation with a dialysed cell extract Propionibacterium freudenreichii ADP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.15 GDP-alpha-D-glucose + D-glucose 6-phosphate in crude extracts of Propionibacterium freudenreichii, OtsA is specific for ADP-glucose, in contrast to the pure recombinant OtsA, which uses UDP-glucose, GDP-glucose and TDP-glucose, in addition to ADP-glucose. The substrate specificity of OtsA in cell extracts is lost during purification, and the recombinant OtsA becomes specific to ADP-glucose upon incubation with a dialysed cell extract Propionibacterium freudenreichii GDP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.15 additional information the trehalose-6-phosphate synthase/phosphatase (OtsA–OtsB) pathway plays an important role in the synthesis of trehalose in response to stress Propionibacterium freudenreichii ?
-
 ?
2.4.1.15 TDP-alpha-D-glucose + D-glucose 6-phosphate in crude extracts of Propionibacterium freudenreichii, OtsA is specific for ADP-glucose, in contrast to the pure recombinant OtsA, which uses UDP-glucose, GDP-glucose and TDP-glucose, in addition to ADP-glucose. The substrate specificity of OtsA in cell extracts is lost during purification, and the recombinant OtsA becomes specific to ADP-glucose upon incubation with a dialysed cell extract Propionibacterium freudenreichii TDP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.15 UDP-alpha-D-glucose + D-glucose 6-phosphate in crude extracts of Propionibacterium freudenreichii, OtsA is specific for ADP-glucose, in contrast to the pure recombinant OtsA, which uses UDP-glucose, GDP-glucose and TDP-glucose, in addition to ADP-glucose. The substrate specificity of OtsA in cell extracts is lost during purification, and the recombinant OtsA becomes specific to ADP-glucose upon incubation with a dialysed cell extract Propionibacterium freudenreichii UDP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.347 ADP-alpha-D-glucose + D-glucose 6-phosphate in crude extracts of Propionibacterium freudenreichii, OtsA is specific for ADP-glucose, in contrast to the pure recombinant OtsA, which uses UDP-glucose, GDP-glucose and TDP-glucose, in addition to ADP-glucose. The substrate specificity of OtsA in cell extracts is lost during purification, and the recombinant OtsA becomes specific to ADP-glucose upon incubation with a dialysed cell extract Propionibacterium freudenreichii ADP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.347 GDP-alpha-D-glucose + D-glucose 6-phosphate in crude extracts of Propionibacterium freudenreichii, OtsA is specific for ADP-glucose, in contrast to the pure recombinant OtsA, which uses UDP-glucose, GDP-glucose and TDP-glucose, in addition to ADP-glucose. The substrate specificity of OtsA in cell extracts is lost during purification, and the recombinant OtsA becomes specific to ADP-glucose upon incubation with a dialysed cell extract Propionibacterium freudenreichii GDP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.347 additional information the trehalose-6-phosphate synthase/phosphatase (OtsA-OtsB) pathway plays an important role in the synthesis of trehalose in response to stress Propionibacterium freudenreichii ?
-
 ?
2.4.1.347 TDP-alpha-D-glucose + D-glucose 6-phosphate in crude extracts of Propionibacterium freudenreichii, OtsA is specific for ADP-glucose, in contrast to the pure recombinant OtsA, which uses UDP-glucose, GDP-glucose and TDP-glucose, in addition to ADP-glucose. The substrate specificity of OtsA in cell extracts is lost during purification, and the recombinant OtsA becomes specific to ADP-glucose upon incubation with a dialysed cell extract Propionibacterium freudenreichii TDP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.347 UDP-alpha-D-glucose + D-glucose 6-phosphate in crude extracts of Propionibacterium freudenreichii, OtsA is specific for ADP-glucose, in contrast to the pure recombinant OtsA, which uses UDP-glucose, GDP-glucose and TDP-glucose, in addition to ADP-glucose. The substrate specificity of OtsA in cell extracts is lost during purification, and the recombinant OtsA becomes specific to ADP-glucose upon incubation with a dialysed cell extract Propionibacterium freudenreichii UDP + alpha,alpha-trehalose 6-phosphate
-
 ?
3.1.3.12 trehalose 6-phosphate + H2O
-
 Propionibacterium freudenreichii subsp. shermanii trehalose + phosphate
-
 ?
3.1.3.12 trehalose 6-phosphate + H2O
-
 Propionibacterium freudenreichii subsp. shermanii NIZO B365 trehalose + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.15 OtsA
-
 Propionibacterium freudenreichii
2.4.1.347 OtsA
-
 Propionibacterium freudenreichii
3.1.3.12 OtsB
-
 Propionibacterium freudenreichii subsp. shermanii
3.1.3.12 TPP
-
 Propionibacterium freudenreichii subsp. shermanii
3.1.3.12 trehalose-6-phosphate phosphatase
-
 Propionibacterium freudenreichii subsp. shermanii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.15 45
-
-
 Propionibacterium freudenreichii
2.4.1.347 45
-
-
 Propionibacterium freudenreichii
3.1.3.12 50
-
-
 Propionibacterium freudenreichii subsp. shermanii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.15 5.3
-
 activity with ADP-glucose, UDP-glucose and TDP-glucose Propionibacterium freudenreichii
2.4.1.15 5.5
-
 activitry with GDP-glucose Propionibacterium freudenreichii
2.4.1.347 5.3
-
 activity with ADP-glucose, UDP-glucose and TDP-glucose Propionibacterium freudenreichii
2.4.1.347 5.5
-
 activitry with GDP-glucose Propionibacterium freudenreichii
3.1.3.12 6.5
-
-
 Propionibacterium freudenreichii subsp. shermanii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.15 5 6 pH 5.0: about 85% of maximal activity, pH 6.0: about 50% of maximal activity Propionibacterium freudenreichii
2.4.1.347 5 6 pH 5.0: about 85% of maximal activity, pH 6.0: about 50% of maximal activity Propionibacterium freudenreichii