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Literature summary extracted from

  • Chen, H.; Hopper, S.L.; Cerniglia, C.E.
    Biochemical and molecular characterization of an azoreductase from Staphylococcus aureus, a tetrameric NADPH-dependent flavoprotein (2005), Microbiology, 151, 1433-1441.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.1.6 expressed in Escherichia coli Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.6 0.057
-
 2-[4-(dimethylamino)phenylazo]benzoic acid in 25 mM potassium phosphate buffer (pH 7.1), 0.25 mM 2-[4-(dimethylamino)phenylazo]benzoic acid and 0.1 mM NADPH Staphylococcus aureus
1.7.1.6 0.074
-
 NADPH in 25 mM potassium phosphate buffer (pH 7.1), 0.25 mM azo dye and 0.1 mM NADPH Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.6 20000
-
 SDS-PAGE Staphylococcus aureus
1.7.1.6 85000
-
 gel filtration Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.6 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.6 HiPrep 16/10 Q XL anion-exchange column chromatography and HiLoad 26/60 Superdex 200 gel fltration Staphylococcus aureus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.6 0.015
-
 crude cell extract Staphylococcus aureus
1.7.1.6 0.14
-
 after purification Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.6 2-[4-(dimethylamino)phenylazo]benzoic acid + NADPH
-
 Staphylococcus aureus N,N-dimethyl-p-phenylenediamine + 2-aminobenzoic acid + NADP+
-
 ?
1.7.1.6 Amaranth + NADPH
-
 Staphylococcus aureus ? + NADP+
-
 ?
1.7.1.6 additional information does not react with NADH as electron donor Staphylococcus aureus ?
-
 ?
1.7.1.6 Orange II + NADPH
-
 Staphylococcus aureus ? + NADP+
-
 ?
1.7.1.6 Ponceau BS + NADPH
-
 Staphylococcus aureus ? + NADP+
-
 ?
1.7.1.6 Ponceau S + NADPH
-
 Staphylococcus aureus ? + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.1.6 tetramer 4 * 20000, SDS-PAGE Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
1.7.1.6 Azo 1
-
 Staphylococcus aureus
1.7.1.6 NADPH-flavin azoreductase
-
 Staphylococcus aureus

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.6 FMN four non-covalently bound FMN Staphylococcus aureus
1.7.1.6 NADPH
-
 Staphylococcus aureus