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Literature summary extracted from

  • Zhao, H.; Chen, D.; Li, Y.; Cai, B.
    Overexpression, purification and characterization of a new salicylate hydroxylase from naphthalene-degrading Pseudomonas sp. strain ND6 (2005), Microbiol. Res., 160, 307-313.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.1 expressed in Escherichia coli strain BL21(DE3) Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.1 0.0005
-
salicylate enzyme form NahG Pseudomonas sp.
1.14.13.1 0.00494
-
salicylate enzyme form NahU Pseudomonas sp.
1.14.13.1 0.02326
-
NADH enzyme form NahG Pseudomonas sp.
1.14.13.1 0.07974
-
NADH enzyme form NahU Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.1 47000
-
SDS-PAGE Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.1 Pseudomonas sp.
-
-
-
1.14.13.1 Pseudomonas sp. ND6
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.1 Ni-NTA His Bind resin column chromatography Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.1 additional information
-
no activity of enzyme form NahU when using 3,5-dinitrosalicylate as substrate Pseudomonas sp.
1.14.13.1 1.27
-
enzyme form NahG, using 3,5-dinitrosalicylate as substrate, at 20°C Pseudomonas sp.
1.14.13.1 1.53
-
enzyme form NahU, using sulfosalicylate as substrate, at 20°C Pseudomonas sp.
1.14.13.1 3.17
-
enzyme form NahG, using salicylate as substrate, at 20°C Pseudomonas sp.
1.14.13.1 3.63
-
enzyme form NahG, using 5-chlorosalicylate as substrate, at 20°C Pseudomonas sp.
1.14.13.1 3.91
-
enzyme form NahG, using 5-methylsalicylate as substrate, at 20°C Pseudomonas sp.
1.14.13.1 4.04
-
enzyme form NahG, using 3-methylsalicylate as substrate, at 20°C Pseudomonas sp.
1.14.13.1 4.12
-
enzyme form NahG, using aspirin as substrate, at 20°C Pseudomonas sp.
1.14.13.1 4.24
-
enzyme form NahG, using sulfosalicylate as substrate, at 20°C Pseudomonas sp.
1.14.13.1 5.25
-
enzyme form NahU, using 5-chlorosalicylate as substrate, at 20°C Pseudomonas sp.
1.14.13.1 10.59
-
enzyme form NahU, using 3-methylsalicylate as substrate, at 20°C Pseudomonas sp.
1.14.13.1 18.79
-
enzyme form NahU, using 5-methylsalicylate as substrate, at 20°C Pseudomonas sp.
1.14.13.1 23.44
-
enzyme form NahU, using salicylate as substrate, at 20°C Pseudomonas sp.
1.14.13.1 25.64
-
enzyme form NahU, using aspirin as substrate, at 20°C Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.1 3,5-dinitrosalicylate + NADH + O2
-
Pseudomonas sp. ? + NAD+ + H2O + CO2
-
?
1.14.13.1 3,5-dinitrosalicylate + NADH + O2
-
Pseudomonas sp. ND6 ? + NAD+ + H2O + CO2
-
?
1.14.13.1 3-methylsalicylate + NADH + O2
-
Pseudomonas sp. 1,2-dihydroxy-3-methylbenzene + NAD+ + H2O + CO2
-
?
1.14.13.1 3-methylsalicylate + NADH + O2
-
Pseudomonas sp. ND6 1,2-dihydroxy-3-methylbenzene + NAD+ + H2O + CO2
-
?
1.14.13.1 5-chlorosalicylate + NADH + O2
-
Pseudomonas sp. 1,2-dihydroxy-5-chlorobenzene + NAD+ + H2O + CO2
-
?
1.14.13.1 5-chlorosalicylate + NADH + O2
-
Pseudomonas sp. ND6 1,2-dihydroxy-5-chlorobenzene + NAD+ + H2O + CO2
-
?
1.14.13.1 5-methylsalicylate + NADH + H+ + O2
-
Pseudomonas sp. 1,2-dihydroxy-5-methylbenzene + CO2 + NAD+ + H2O
-
?
1.14.13.1 5-methylsalicylate + NADH + H+ + O2
-
Pseudomonas sp. ND6 1,2-dihydroxy-5-methylbenzene + CO2 + NAD+ + H2O
-
?
1.14.13.1 aspirin + NADH + O2
-
Pseudomonas sp. ? + NAD+ + H2O + CO2
-
?
1.14.13.1 salicylate + NADH + H+ + O2
-
Pseudomonas sp. catechol + NAD+ + H2O + CO2
-
?
1.14.13.1 salicylate + NADH + H+ + O2
-
Pseudomonas sp. ND6 catechol + NAD+ + H2O + CO2
-
?
1.14.13.1 sulfosalicylate + NADH + O2
-
Pseudomonas sp. ? + NAD+ + H2O + CO2
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.1 NahG
-
Pseudomonas sp.
1.14.13.1 NahU
-
Pseudomonas sp.
1.14.13.1 salicylate hydroxylase
-
Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.1 FAD
-
Pseudomonas sp.
1.14.13.1 NADH
-
Pseudomonas sp.