EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.1 | expressed in Escherichia coli strain BL21(DE3) | Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.1 | 0.0005 | - |
salicylate | enzyme form NahG | Pseudomonas sp. | |
1.14.13.1 | 0.00494 | - |
salicylate | enzyme form NahU | Pseudomonas sp. | |
1.14.13.1 | 0.02326 | - |
NADH | enzyme form NahG | Pseudomonas sp. | |
1.14.13.1 | 0.07974 | - |
NADH | enzyme form NahU | Pseudomonas sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.13.1 | 47000 | - |
SDS-PAGE | Pseudomonas sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.1 | Pseudomonas sp. | - |
- |
- |
1.14.13.1 | Pseudomonas sp. ND6 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.1 | Ni-NTA His Bind resin column chromatography | Pseudomonas sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.13.1 | additional information | - |
no activity of enzyme form NahU when using 3,5-dinitrosalicylate as substrate | Pseudomonas sp. |
1.14.13.1 | 1.27 | - |
enzyme form NahG, using 3,5-dinitrosalicylate as substrate, at 20°C | Pseudomonas sp. |
1.14.13.1 | 1.53 | - |
enzyme form NahU, using sulfosalicylate as substrate, at 20°C | Pseudomonas sp. |
1.14.13.1 | 3.17 | - |
enzyme form NahG, using salicylate as substrate, at 20°C | Pseudomonas sp. |
1.14.13.1 | 3.63 | - |
enzyme form NahG, using 5-chlorosalicylate as substrate, at 20°C | Pseudomonas sp. |
1.14.13.1 | 3.91 | - |
enzyme form NahG, using 5-methylsalicylate as substrate, at 20°C | Pseudomonas sp. |
1.14.13.1 | 4.04 | - |
enzyme form NahG, using 3-methylsalicylate as substrate, at 20°C | Pseudomonas sp. |
1.14.13.1 | 4.12 | - |
enzyme form NahG, using aspirin as substrate, at 20°C | Pseudomonas sp. |
1.14.13.1 | 4.24 | - |
enzyme form NahG, using sulfosalicylate as substrate, at 20°C | Pseudomonas sp. |
1.14.13.1 | 5.25 | - |
enzyme form NahU, using 5-chlorosalicylate as substrate, at 20°C | Pseudomonas sp. |
1.14.13.1 | 10.59 | - |
enzyme form NahU, using 3-methylsalicylate as substrate, at 20°C | Pseudomonas sp. |
1.14.13.1 | 18.79 | - |
enzyme form NahU, using 5-methylsalicylate as substrate, at 20°C | Pseudomonas sp. |
1.14.13.1 | 23.44 | - |
enzyme form NahU, using salicylate as substrate, at 20°C | Pseudomonas sp. |
1.14.13.1 | 25.64 | - |
enzyme form NahU, using aspirin as substrate, at 20°C | Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.1 | 3,5-dinitrosalicylate + NADH + O2 | - |
Pseudomonas sp. | ? + NAD+ + H2O + CO2 | - |
? | |
1.14.13.1 | 3,5-dinitrosalicylate + NADH + O2 | - |
Pseudomonas sp. ND6 | ? + NAD+ + H2O + CO2 | - |
? | |
1.14.13.1 | 3-methylsalicylate + NADH + O2 | - |
Pseudomonas sp. | 1,2-dihydroxy-3-methylbenzene + NAD+ + H2O + CO2 | - |
? | |
1.14.13.1 | 3-methylsalicylate + NADH + O2 | - |
Pseudomonas sp. ND6 | 1,2-dihydroxy-3-methylbenzene + NAD+ + H2O + CO2 | - |
? | |
1.14.13.1 | 5-chlorosalicylate + NADH + O2 | - |
Pseudomonas sp. | 1,2-dihydroxy-5-chlorobenzene + NAD+ + H2O + CO2 | - |
? | |
1.14.13.1 | 5-chlorosalicylate + NADH + O2 | - |
Pseudomonas sp. ND6 | 1,2-dihydroxy-5-chlorobenzene + NAD+ + H2O + CO2 | - |
? | |
1.14.13.1 | 5-methylsalicylate + NADH + H+ + O2 | - |
Pseudomonas sp. | 1,2-dihydroxy-5-methylbenzene + CO2 + NAD+ + H2O | - |
? | |
1.14.13.1 | 5-methylsalicylate + NADH + H+ + O2 | - |
Pseudomonas sp. ND6 | 1,2-dihydroxy-5-methylbenzene + CO2 + NAD+ + H2O | - |
? | |
1.14.13.1 | aspirin + NADH + O2 | - |
Pseudomonas sp. | ? + NAD+ + H2O + CO2 | - |
? | |
1.14.13.1 | salicylate + NADH + H+ + O2 | - |
Pseudomonas sp. | catechol + NAD+ + H2O + CO2 | - |
? | |
1.14.13.1 | salicylate + NADH + H+ + O2 | - |
Pseudomonas sp. ND6 | catechol + NAD+ + H2O + CO2 | - |
? | |
1.14.13.1 | sulfosalicylate + NADH + O2 | - |
Pseudomonas sp. | ? + NAD+ + H2O + CO2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.1 | NahG | - |
Pseudomonas sp. |
1.14.13.1 | NahU | - |
Pseudomonas sp. |
1.14.13.1 | salicylate hydroxylase | - |
Pseudomonas sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.1 | FAD | - |
Pseudomonas sp. | |
1.14.13.1 | NADH | - |
Pseudomonas sp. |