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Literature summary extracted from

  • Dupret, J.M.; Dairou, J.; Atmane, N.; Rodrigues-Lima, F.
    Inactivation of human arylamine N-acetyltransferase 1 by hydrogen peroxide and peroxynitrite (2005), Methods Enzymol., 400, 215-229.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.5 expression of His-tagged isozyme NAT1 in Escherichia coli strain BL21(DE3) Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.5 hydrogen peroxide inactivates isozyme NAT1, in vivo effect, overview Homo sapiens
2.3.1.5 peroxynitrite inactivates isozyme NAT1, in vivo effect, overview Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.5 acetyl-CoA + an arylamine Homo sapiens NATs are xenobiotic metabolizing enzymes responsible for the acetylation of many arylamine and heterocyclic amines, they therefore play an important role in the detoxification and activation of numerous drugs and carcinogens, regulation, overview CoA + an N-acetylarylamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.5 Homo sapiens P11245 isozyme NAT2; isozyme NAT2
-
2.3.1.5 Homo sapiens P18440 isozyme NAT1; isozyme NAT1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.5 recombinant His-tagged isozyme NAT1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.5 intestine isozyme NAT2 mainly Homo sapiens
-
2.3.1.5 liver isozyme NAT2 mainly Homo sapiens
-
2.3.1.5 MCF-7 cell mammary carcinoma cell line, NAT1 Homo sapiens
-
2.3.1.5 additional information isozyme NAT1 shows a broad tissue distribution Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.5 4-nitrophenyl acetate + 5-aminosalicylic acid
-
 Homo sapiens 4-nitrophenol + 5-acetylaminosalicylic acid
-
 ?
2.3.1.5 acetyl-CoA + 4-dimethylaminobenzaldehyde
-
 Homo sapiens CoA + 5-acetyl-4-dimethylaminobenzaldehyde
-
 ?
2.3.1.5 acetyl-CoA + 5-aminosalicylic acid
-
 Homo sapiens CoA + 5-acetylaminosalicylic acid
-
 ?
2.3.1.5 acetyl-CoA + an arylamine
-
 Homo sapiens CoA + an N-acetylarylamine
-
 ?
2.3.1.5 acetyl-CoA + an arylamine NATs are xenobiotic metabolizing enzymes responsible for the acetylation of many arylamine and heterocyclic amines, they therefore play an important role in the detoxification and activation of numerous drugs and carcinogens, regulation, overview Homo sapiens CoA + an N-acetylarylamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.5 NAT
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.5 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.5 7.5
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.5 acetyl-CoA
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.5 additional information
-
 additional information inactivation kinetics of NAT1 by oxidants, overview Homo sapiens