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Literature summary extracted from

  • Barrios, A.; Selleck, W.; Hnatkovich, B.; Kramer, R.; Sermwittayawong, D.; Tan, S.
    Expression and purification of recombinant yeast Ada2/Ada3/Gcn5 and Piccolo NuA4 histone acetyltransferase complexes (2007), Methods, 41, 271-277.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.48 expression of the His-tagged enzyme bound in the Ada2/Ada3/Gcn5 subcomplex of SAGA or the Piccolo NuA4 complex in Escherichia coli strain BL21(DE3), polycistronic vector, method development, overview Saccharomyces cerevisiae
2.3.1.48 successful expression using Escherichia coli BL-21 Saccharomyces cerevisiae
2.3.1.48 successful expression using Escherichia coli BL-21 by using a His-tagged Ada3 subunit of the yeast Ada2/Ada3/Gcn5 complex Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.48 nucleus
-
 Saccharomyces cerevisiae 5634
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.48 43000
-
 x * 43000, recombinant Ada2/Ada3/Gcn5-enzyme complex, SDS-PAGE, x * 43200, recombinant Piccolo NuA4-enzyme complex, SDS-PAGE Saccharomyces cerevisiae
2.3.1.48 43200
-
 x * 43000, recombinant Ada2/Ada3/Gcn5-enzyme complex, SDS-PAGE, x * 43200, recombinant Piccolo NuA4-enzyme complex, SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.48 histone + acetyl-CoA Saccharomyces cerevisiae key enzyme in post-translational modification of histones associated with transcriptionally active genes acetyl-histone + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.48 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.48 recombinant His-tagged enzyme in Ada2/Ada3/Gcn5 subcomplex of SAGA or in the Piccolo NuA4 complex from Escherichia coli strain BL21(DE3) by metal affinity chromatography followed by anion or cation exchange chromatography, and hydrophobicinteraction chromatography, method development, overview Saccharomyces cerevisiae
2.3.1.48 using talon metal affinity chromatography, anion-exchange chromatography and cation-exchange chromatography. Typical yields of the yeast Ada2/Ada3/Gcn5 complex are approximately 0.5-0.8 mg/L of culture Saccharomyces cerevisiae
2.3.1.48 using talon metal affinity chromatography, anion-exchange chromatography, cation-exchange chromatography and hydrophobic interaction chromatography. Typical yields of the Piccolo NuA4 complex are of the 1-3 mg per liter of culture Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.48 histone + acetyl-CoA key enzyme in post-translational modification of histones associated with transcriptionally active genes Saccharomyces cerevisiae acetyl-histone + CoA
-
 ?
2.3.1.48 histone H2A + acetyl-CoA acetylation of the tail of the histone, the enzyme is organized in the NuA4 subcomplex acting on the nucleosome, overview Saccharomyces cerevisiae acetyl-histone H2A + CoA
-
 ?
2.3.1.48 histone H2B + acetyl-CoA acetylation of the tail of the histone, the enzyme is organized in the catalytic Ada2/Ada3/Gcn5 subcomplex of SAGA acting on the nucleosome, overview Saccharomyces cerevisiae acetyl-histone H2B + CoA
-
 ?
2.3.1.48 histone H3 + acetyl-CoA acetylation of the tail of the histone, the enzyme is organized in the catalytic Ada2/Ada3/Gcn5 subcomplex of SAGA acting on the nucleosome, overview Saccharomyces cerevisiae acetyl-histone H3 + CoA
-
 ?
2.3.1.48 histone H4 + acetyl-CoA acetylation of the tail of the histone, the enzyme is organized in the NuA4 subcomplex acting on the nucleosome, overview Saccharomyces cerevisiae acetyl-histone H4 + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.48 ? x * 43000, recombinant Ada2/Ada3/Gcn5-enzyme complex, SDS-PAGE, x * 43200, recombinant Piccolo NuA4-enzyme complex, SDS-PAGE Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
2.3.1.48 Ada2/Ada3/Gcn5 complex
-
 Saccharomyces cerevisiae
2.3.1.48 HAT
-
 Saccharomyces cerevisiae
2.3.1.48 histone acetyltransferase
-
 Saccharomyces cerevisiae
2.3.1.48 Piccolo NuA4 complex
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.48 acetyl-CoA
-
 Saccharomyces cerevisiae