EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.3.5 | the chromosomal gene encoding the 2-methyl-cis-aconitate hydratase is disrupted by direct insertion of a copy of an additional 2-methylcitrate synthase gene (prpc). 2-Methylcitrate synthase is expressed under the control of the constitutive kanamycin-resistance gene resulting in up to 20fold higher specific 2-methylcitrate synthase activities in comparison to the wild-type. The disruption of the acnM gene by insertion of prpC leads to a propionate- and levulinate-negative phenotype of the engineered strains and accumulation of 2-methylcitrate in the medium | Cupriavidus necator |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.3.5 | Cupriavidus necator | - |
- |
- |
2.3.3.5 | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | - |
- |
- |