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Literature summary extracted from

  • Müller, U.; van Assema, F.; Gunsior, M.; Orf, S.; Kremer, S.; Schipper, D.; Wagemans, A.; Townsend, C.A.; Sonke, T.; Bovenberg, R.; Wubbolts, M.
    Metabolic engineering of the E. coli L-phenylalanine pathway for the production of D-phenylglycine (D-Phg) (2006), Metab. Eng., 8, 196-208.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.6.1.72 synthesis metabolic engineering of the Escherichia coli L-phenylalanine pathway for the production of D-phenylglycine. Expression of hydroxymandelate synthase from Amycolatopsis orientalis, hydroxymandelate oxidase from Streptomyces coelicolor and hydroxyphenylglycine aminotransferase from Pseudomonas putida in Escherichia coli Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.46 expressed in Escherichia coli Top10 cells Streptomyces coelicolor
1.1.3.46 expressed in Escherichia coli Top10 cells Amycolatopsis orientalis
2.6.1.72 expression in Escherichia coli Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.46 (S)-4-hydroxymandelate + O2 Streptomyces coelicolor
-
 2-(4-hydroxyphenyl)-2-oxoacetate + H2O2
-
 ?
1.1.3.46 (S)-4-hydroxymandelate + O2 Amycolatopsis orientalis
-
 2-(4-hydroxyphenyl)-2-oxoacetate + H2O2
-
 ?
2.6.1.72 4-hydroxyphenylglyoxylate + L-glutamate Pseudomonas putida enzyme is involved in biosynthesis of D-4-hydroxyphenylglycine D-4-hydroxyphenylglycine + 2-oxoglutarate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.46 Amycolatopsis orientalis O52791
-
-
1.1.3.46 Streptomyces coelicolor
-
-
-
2.6.1.72 Pseudomonas putida
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.46 (S)-4-hydroxymandelate + O2
-
 Streptomyces coelicolor 2-(4-hydroxyphenyl)-2-oxoacetate + H2O2
-
 ?
1.1.3.46 (S)-4-hydroxymandelate + O2
-
 Amycolatopsis orientalis 2-(4-hydroxyphenyl)-2-oxoacetate + H2O2
-
 ?
1.1.3.46 4-hydroxyphenylpyruvate + O2
-
 Streptomyces coelicolor phenylpyruvate + H2O2
-
 ?
1.1.3.46 4-hydroxyphenylpyruvate + O2
-
 Amycolatopsis orientalis phenylpyruvate + H2O2
-
 ?
2.6.1.72 4-hydroxyphenylglyoxylate + L-glutamate enzyme is involved in biosynthesis of D-4-hydroxyphenylglycine Pseudomonas putida D-4-hydroxyphenylglycine + 2-oxoglutarate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.46 HmO
-
 Streptomyces coelicolor
1.1.3.46 HmO
-
 Amycolatopsis orientalis
1.1.3.46 hydroxymandelate oxidase
-
 Streptomyces coelicolor
1.1.3.46 hydroxymandelate oxidase
-
 Amycolatopsis orientalis
2.6.1.72 HpgAT
-
 Pseudomonas putida
2.6.1.72 hydroxyphenylglycine aminotransferase
-
 Pseudomonas putida