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Literature summary extracted from

  • Gitter, S.J.; Cooper, C.L.; Friesen, J.A.; Jones, M.A.
    Investigation of the catalytic and structural roles of conserved histidines of human coproporphyrinogen oxidase using site-directed mutagenesis (2006), Med. Sci. Monit., 13, BR1-BR10.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.3.3.3 medicine His158 may have a role in the active site of CPO, none of the conserved histidine residues of the enzyme are essential for catalytic activity although changes in histidines are implicated in the disease state hereditary coproporphyria Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.3.3 expressed in Escherichia coli strain BL21(DE3)RIL Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.3.3.3 H148A retains 39% of wild type enzyme activity for the overall conversion of coproporphyrinogen-III to protoporphyrinogen-IX Homo sapiens
1.3.3.3 H158A the mutant exhibits approximately 50fold lower activity than wild type recombinant CPO for the conversion of coproporphyrinogen-III to protoporphyrinogen-IX Homo sapiens
1.3.3.3 H197A the mutant exhibits approximately 50fold lower activity for the overall conversion of coproporphyrinogen-III to protoporphyrinogen-IX than wild type recombinant CPO, but the second oxidative decarboxylation step is not impaired, with mutant enzyme H197A retaining 100% of the wild type activity using harderoporphyrinogen as substrate Homo sapiens
1.3.3.3 H227A catalyzes the conversion of coproporphyrinogen-III to protoporphyrinogen-IX at a rate almost 2fold that of the wild type enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.3.3 0.00015
-
 Coproporphyrinogen-III mutant enzyme H158A, at 37°C Homo sapiens
1.3.3.3 0.00015
-
 Harderoporphyrinogen mutant enzyme H158A, at 37°C Homo sapiens
1.3.3.3 0.0003
-
 Coproporphyrinogen-III wild type enzyme, at 37°C Homo sapiens
1.3.3.3 0.0012
-
 Harderoporphyrinogen wild type enzyme, at 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.3 Homo sapiens P36551 precursor
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.3 TALON metal affinity resin column chromatography Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.3.3 0.0017
-
 wild type enzyme, for mesoporphyrinogen-VI, at 37°C Homo sapiens
1.3.3.3 0.0049
-
 wild type enzyme, for coproporphyrinogen-III, at 37°C Homo sapiens
1.3.3.3 0.0051
-
 wild type enzyme, for harderoporphyrinogen, at 37°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.3 coproporphyrinogen-III + O2 + 2 H+
-
 Homo sapiens protoporphyrinogen-IX + 2 CO2 + 2 H2O
-
 ?
1.3.3.3 coproporphyrinogen-III + O2 + 2 H+
-
 Homo sapiens harderoporphyrinogen + CO2 + 2 H2O
-
 ?
1.3.3.3 harderoporphyrinogen + O2 + 2 H+
-
 Homo sapiens protoporphyrinogen-IX + CO2 + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.3.3 CPO
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.3.3 0.00025
-
 Coproporphyrinogen-III mutant H158A Homo sapiens
1.3.3.3 0.000383
-
 Harderoporphyrinogen mutant H158A Homo sapiens
1.3.3.3 0.06
-
 Coproporphyrinogen-III wild-type Homo sapiens
1.3.3.3 0.1317
-
 Harderoporphyrinogen wild-type Homo sapiens