Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lung, S.C.; Weselake, R.J.
    Diacylglycerol acyltransferase: a key mediator of plant triacylglycerol synthesis (2006), Lipids, 41, 1073-1088.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.20 ATP
-
 Brassica napus
2.3.1.20 bovine serum albumin 5fold at 3-4 mg/ml Brassica napus
2.3.1.20 CoA
-
 Brassica napus
2.3.1.20 additional information effectivity of detergents used in in vitro assays, overview Spinacia oleracea
2.3.1.20 additional information effectivity of detergents used in in vitro assays, overview Zea mays
2.3.1.20 additional information effectivity of detergents used in in vitro assays, overview Glycine max
2.3.1.20 additional information effectivity of detergents used in in vitro assays, overview Umbelopsis ramanniana
2.3.1.20 additional information effectivity of detergents used in in vitro assays, overview Ricinus communis
2.3.1.20 additional information effectivity of detergents used in in vitro assays, overview Mus musculus
2.3.1.20 additional information effectivity of detergents used in in vitro assays, overview Brassica napus
2.3.1.20 additional information effectivity of detergents used in in vitro assays, overview Cuphea sp.
2.3.1.20 additional information effectivity of detergents used in in vitro assays, overview Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.20 DGAT1, a single copy gene, DNA and amino acid sequence determination and analysis, phylogenetic tree, functional overexpression in tobacco plants Arabidopsis thaliana
2.3.1.20 DGAT1, DNA and amino acid sequence determination and analysis Arachis hypogaea
2.3.1.20 DGAT1, DNA and amino acid sequence determination and analysis Olea europaea
2.3.1.20 DGAT1, DNA and amino acid sequence determination and analysis, phylogenetic tree Ricinus communis
2.3.1.20 DGAT1, DNA and amino acid sequence determination and analysis, phylogenetic tree Brassica napus
2.3.1.20 DGAT1, DNA and amino acid sequence determination and analysis, phylogenetic tree Nicotiana tabacum
2.3.1.20 DGAT2, DNA and amino acid sequence determination and analysis, DGAT2 is a truncated enzyme version, phylogenetic tree Brassica napus
2.3.1.20 DNA and amino acid sequence determination and analysis Acinetobacter calcoaceticus
2.3.1.20 DNA and amino acid sequence determination and analysis, phylogenetic tree Carthamus tinctorius
2.3.1.20 DNA and amino acid sequence determination and analysis, phylogenetic tree, expression in insect cells Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.20 additional information a recombinant acyl-CoA binding protein increased the activity of Arabidopsis thaliana DGAT1 expressed in insect cell culture by up to 70%, overexpression of AtDGAT1 cDNA from Arabidopsis does not complement the knocked-out acyl-CoA:cholesterol acyltransferases, ACAT, EC 2.3.1.26, in a yeast double-mutant Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.20 2-Bromooctanoate
-
 Brassica napus
2.3.1.20 bovine serum albumin strong inhibition Carthamus tinctorius
2.3.1.20 Ca2+
-
 Carthamus tinctorius
2.3.1.20 Hg2+
-
 Carthamus tinctorius
2.3.1.20 Iodide
-
 Brassica napus
2.3.1.20 Mg2+
-
 Carthamus tinctorius
2.3.1.20 niacin
-
 Arabidopsis thaliana
2.3.1.20 Soybean trypsin inhibitor
-
 Brassica napus
2.3.1.20 tannin
-
 Brassica napus
2.3.1.20 Zn2+
-
 Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.20 chloroplast thylakoid and envelope membranes, not in the stroma Arabidopsis thaliana 9507
-
2.3.1.20 cytosol
-
 Arachis hypogaea 5829
-
2.3.1.20 endoplasmic reticulum
-
 Spinacia oleracea 5783
-
2.3.1.20 endoplasmic reticulum
-
 Glycine max 5783
-
2.3.1.20 endoplasmic reticulum
-
 Umbelopsis ramanniana 5783
-
2.3.1.20 endoplasmic reticulum
-
 Ricinus communis 5783
-
2.3.1.20 endoplasmic reticulum
-
 Mus musculus 5783
-
2.3.1.20 endoplasmic reticulum
-
 Brassica napus 5783
-
2.3.1.20 endoplasmic reticulum
-
 Cuphea sp. 5783
-
2.3.1.20 membrane
-
 Rattus norvegicus 16020
-
2.3.1.20 membrane
-
 Zea mays 16020
-
2.3.1.20 membrane
-
 Acinetobacter calcoaceticus 16020
-
2.3.1.20 membrane
-
 Carthamus tinctorius 16020
-
2.3.1.20 membrane
-
 Nicotiana tabacum 16020
-
2.3.1.20 microsome
-
 Rattus norvegicus
-
-
2.3.1.20 microsome
-
 Carthamus tinctorius
-
-
2.3.1.20 microsome
-
 Ricinus communis
-
-
2.3.1.20 microsome
-
 Brassica napus
-
-
2.3.1.20 microsome
-
 Cuphea sp.
-
-
2.3.1.20 oil body
-
 Carthamus tinctorius
-
-
2.3.1.20 oil body
-
 Umbelopsis ramanniana
-
-
2.3.1.20 rough endoplasmic reticulum
-
 Zea mays 5791
-
2.3.1.20 thylakoid membrane
-
 Arabidopsis thaliana 42651
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.20 Mg2+
-
 Rattus norvegicus
2.3.1.20 Mg2+
-
 Zea mays
2.3.1.20 Mg2+
-
 Carthamus tinctorius
2.3.1.20 Mg2+
-
 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.20 1,2-diacylglycerol + acyl-CoA Rattus norvegicus
-
 triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA Spinacia oleracea
-
 triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA Zea mays
-
 triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA Glycine max
-
 triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA Acinetobacter calcoaceticus
-
 triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA Arachis hypogaea
-
 triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA Carthamus tinctorius
-
 triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA Umbelopsis ramanniana
-
 triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA Olea europaea
-
 triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA Ricinus communis
-
 triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA Mus musculus
-
 triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA Cuphea sp.
-
 triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA Nicotiana tabacum
-
 triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA Brassica napus DGAT catalyzes the transfer of an acyl moiety between two DAG molecules to form triacylglycerol and monoacylglycerol triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA Arabidopsis thaliana role in leaf metabolism, overview triacylglycerol + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Acinetobacter calcoaceticus
-
-
-
2.3.1.20 Arabidopsis thaliana
-
-
-
2.3.1.20 Arabidopsis thaliana Q9SLD2
-
-
2.3.1.20 Arachis hypogaea
-
-
-
2.3.1.20 Brassica napus
-
-
-
2.3.1.20 Brassica napus Q9XGR5 DGAT1
-
2.3.1.20 Brassica napus Q9XGV4 DGAT2
-
2.3.1.20 Carthamus tinctorius
-
-
-
2.3.1.20 Cuphea sp.
-
-
-
2.3.1.20 Glycine max
-
-
-
2.3.1.20 Mus musculus
-
-
-
2.3.1.20 Mus musculus Q9Z2A7 DGAT1
-
2.3.1.20 Nicotiana tabacum Q9SEG9
-
-
2.3.1.20 Olea europaea
-
-
-
2.3.1.20 Rattus norvegicus
-
-
-
2.3.1.20 Ricinus communis
-
-
-
2.3.1.20 Ricinus communis Q67C39
-
-
2.3.1.20 Spinacia oleracea
-
-
-
2.3.1.20 Umbelopsis ramanniana
-
-
-
2.3.1.20 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.20 native enzyme from cotyledons to near homogeneity Glycine max
2.3.1.20 native enzyme from the oil body fraction to homogeneity Umbelopsis ramanniana
2.3.1.20 native intestinal enzyme Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.20 cotyledon
-
 Glycine max
-
2.3.1.20 endosperm
-
 Ricinus communis
-
2.3.1.20 fruit
-
 Olea europaea
-
2.3.1.20 intestine
-
 Rattus norvegicus
-
2.3.1.20 leaf
-
 Spinacia oleracea
-
2.3.1.20 leaf
-
 Arabidopsis thaliana
-
2.3.1.20 leaf
-
 Nicotiana tabacum
-
2.3.1.20 liver
-
 Rattus norvegicus
-
2.3.1.20 seed
-
 Ricinus communis
-
2.3.1.20 seed
-
 Cuphea sp.
-
2.3.1.20 seed developing Glycine max
-
2.3.1.20 seed maturing Carthamus tinctorius
-
2.3.1.20 seed developing oilseed Brassica napus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 1,2-diacylglycerol + acyl-CoA
-
 Rattus norvegicus triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA
-
 Spinacia oleracea triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA
-
 Zea mays triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA
-
 Glycine max triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA
-
 Acinetobacter calcoaceticus triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA
-
 Arachis hypogaea triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA
-
 Carthamus tinctorius triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA
-
 Umbelopsis ramanniana triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA
-
 Olea europaea triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA
-
 Ricinus communis triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA
-
 Mus musculus triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA
-
 Brassica napus triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA
-
 Cuphea sp. triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA
-
 Arabidopsis thaliana triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA
-
 Nicotiana tabacum triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA DGAT catalyzes the transfer of an acyl moiety between two DAG molecules to form triacylglycerol and monoacylglycerol Brassica napus triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA role in leaf metabolism, overview Arabidopsis thaliana triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA acyl-CoA specificity, no acylation activity with phosphoatidylcholine or oleic acid, overview Umbelopsis ramanniana triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA acyl-CoA specificity, overview Spinacia oleracea triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA acyl-CoA specificity, overview Zea mays triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA acyl-CoA specificity, overview Glycine max triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA acyl-CoA specificity, overview Olea europaea triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA acyl-CoA specificity, overview Mus musculus triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA microsomal DGAT from castor bean exhibited high activity toward diacylglycerols containing unusual fatty acids, e.g. ricinoleic acids, at both sn-1 and sn-2 positions, acyl-CoA specificity, overview Ricinus communis triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA microsomal DGAT from Cuphea exhibits high activity toward diacylglycerols containing unusual fatty acids, e.g. lauric acids, at both sn-1 and sn-2 positions, acyl-CoA specificity, overview Cuphea sp. triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + erucoyl-CoA
-
 Brassica napus 1,2-diacyl-3-erucoylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + oleoyl-CoA
-
 Brassica napus 1,2-diacyl-3-oleoylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + palmitoyl-CoA
-
 Brassica napus 1,2-diacyl-3-palmitoylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + stearoyl-CoA
-
 Brassica napus 1,2-diacyl-3-stearoylglycerol + CoA
-
 ?
2.3.1.20 1-palmitoyl-2-oleoyl glycerol + acyl-CoA
-
 Mus musculus 1-palmitoyl-2-oleoyl-3-acylglycerol + CoA
-
 ?
2.3.1.20 1-palmitoyl-2-oleoyl glycerol + acyl-CoA
-
 Rattus norvegicus 1-palmitoyl-2-oleoyl-3-acylglycerol + CoA
-
 ?
2.3.1.20 1-palmitoyl-2-oleoyl glycerol + acyl-CoA
-
 Spinacia oleracea 1-palmitoyl-2-oleoyl-3-acylglycerol + CoA
-
 ?
2.3.1.20 1-palmitoyl-2-oleoyl glycerol + acyl-CoA
-
 Zea mays 1-palmitoyl-2-oleoyl-3-acylglycerol + CoA
-
 ?
2.3.1.20 1-palmitoyl-2-oleoyl glycerol + acyl-CoA
-
 Glycine max 1-palmitoyl-2-oleoyl-3-acylglycerol + CoA
-
 ?
2.3.1.20 1-palmitoyl-2-oleoyl glycerol + acyl-CoA
-
 Arabidopsis thaliana 1-palmitoyl-2-oleoyl-3-acylglycerol + CoA
-
 ?
2.3.1.20 1-palmitoyl-2-oleoyl glycerol + acyl-CoA
-
 Ricinus communis 1-palmitoyl-2-oleoyl-3-acylglycerol + CoA
-
 ?
2.3.1.20 1-palmitoyl-2-oleoyl glycerol + acyl-CoA
-
 Carthamus tinctorius 1-palmitoyl-2-oleoyl-3-acylglycerol + CoA
-
 ?
2.3.1.20 1-palmitoyl-2-oleoyl glycerol + acyl-CoA
-
 Umbelopsis ramanniana 1-palmitoyl-2-oleoyl-3-acylglycerol + CoA
-
 ?
2.3.1.20 1-palmitoyl-2-oleoyl glycerol + acyl-CoA
-
 Olea europaea 1-palmitoyl-2-oleoyl-3-acylglycerol + CoA
-
 ?
2.3.1.20 1-palmitoyl-2-oleoyl glycerol + acyl-CoA
-
 Cuphea sp. 1-palmitoyl-2-oleoyl-3-acylglycerol + CoA
-
 ?
2.3.1.20 1-palmitoyl-2-oleoylglycerol + acyl-CoA
-
 Brassica napus 1-palmitoyl-2-oleoyl-3-acylglycerol + CoA
-
 ?
2.3.1.20 2,3-diacylglycerol + acyl-CoA
-
 Rattus norvegicus triacylglycerol + CoA
-
 ?
2.3.1.20 2,3-diacylglycerol + acyl-CoA
-
 Carthamus tinctorius triacylglycerol + CoA
-
 ?
2.3.1.20 diolein + acyl-CoA
-
 Mus musculus ?
-
 ?
2.3.1.20 diolein + acyl-CoA
-
 Rattus norvegicus ?
-
 ?
2.3.1.20 diolein + acyl-CoA
-
 Spinacia oleracea ?
-
 ?
2.3.1.20 diolein + acyl-CoA
-
 Zea mays ?
-
 ?
2.3.1.20 diolein + acyl-CoA
-
 Glycine max ?
-
 ?
2.3.1.20 diolein + acyl-CoA
-
 Arabidopsis thaliana ?
-
 ?
2.3.1.20 diolein + acyl-CoA
-
 Brassica napus ?
-
 ?
2.3.1.20 diolein + acyl-CoA
-
 Ricinus communis ?
-
 ?
2.3.1.20 diolein + acyl-CoA
-
 Carthamus tinctorius ?
-
 ?
2.3.1.20 diolein + acyl-CoA
-
 Umbelopsis ramanniana ?
-
 ?
2.3.1.20 diolein + acyl-CoA
-
 Olea europaea ?
-
 ?
2.3.1.20 diolein + acyl-CoA
-
 Cuphea sp. ?
-
 ?
2.3.1.20 dipalmitin + acyl-CoA
-
 Mus musculus ?
-
 ?
2.3.1.20 dipalmitin + acyl-CoA
-
 Rattus norvegicus ?
-
 ?
2.3.1.20 dipalmitin + acyl-CoA
-
 Spinacia oleracea ?
-
 ?
2.3.1.20 dipalmitin + acyl-CoA
-
 Zea mays ?
-
 ?
2.3.1.20 dipalmitin + acyl-CoA
-
 Glycine max ?
-
 ?
2.3.1.20 dipalmitin + acyl-CoA
-
 Arabidopsis thaliana ?
-
 ?
2.3.1.20 dipalmitin + acyl-CoA
-
 Brassica napus ?
-
 ?
2.3.1.20 dipalmitin + acyl-CoA
-
 Ricinus communis ?
-
 ?
2.3.1.20 dipalmitin + acyl-CoA
-
 Carthamus tinctorius ?
-
 ?
2.3.1.20 dipalmitin + acyl-CoA
-
 Umbelopsis ramanniana ?
-
 ?
2.3.1.20 dipalmitin + acyl-CoA
-
 Olea europaea ?
-
 ?
2.3.1.20 dipalmitin + acyl-CoA
-
 Cuphea sp. ?
-
 ?
2.3.1.20 additional information acyl-CoA specificity, overview Brassica napus ?
-
 ?
2.3.1.20 additional information acyl-CoA specificity, overview Arabidopsis thaliana ?
-
 ?
2.3.1.20 additional information DGAT is also catalyzing the reactions of the monoacylglycerol transferase, EC 2.3.1.22 Umbelopsis ramanniana ?
-
 ?
2.3.1.20 additional information DGAT is catalyzing the reactions of the monoacylglycerol transferase, EC 2.3.1.22, of the diacylglycerol transferase, EC 2.3.1.20, of the wax synthase, EC 2.3.1.75, and of the acyl-CoA:retinol acyltransferase, EC 2.3.1.76, overview Mus musculus ?
-
 ?
2.3.1.20 additional information no activity with sn-1,3-diacylglycerols, acyl-CoA specificity, overview Rattus norvegicus ?
-
 ?
2.3.1.20 additional information no activity with sn-1,3-diacylglycerols, water-soluble diacylglycerols, including rac-1,2-diacetylglycerol and rac-1,2-dibutyrylglycerol, are poor substrates of DGAT from safflower seeds, acyl-CoA specificity, overview Carthamus tinctorius ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.20 trimer
-
 Glycine max

Synonyms

EC Number Synonyms Comment Organism
2.3.1.20 DGAT
-
 Rattus norvegicus
2.3.1.20 DGAT
-
 Spinacia oleracea
2.3.1.20 DGAT
-
 Zea mays
2.3.1.20 DGAT
-
 Glycine max
2.3.1.20 DGAT
-
 Acinetobacter calcoaceticus
2.3.1.20 DGAT
-
 Arachis hypogaea
2.3.1.20 DGAT
-
 Carthamus tinctorius
2.3.1.20 DGAT
-
 Umbelopsis ramanniana
2.3.1.20 DGAT
-
 Olea europaea
2.3.1.20 DGAT
-
 Ricinus communis
2.3.1.20 DGAT
-
 Mus musculus
2.3.1.20 DGAT
-
 Brassica napus
2.3.1.20 DGAT
-
 Cuphea sp.
2.3.1.20 DGAT
-
 Arabidopsis thaliana
2.3.1.20 DGAT
-
 Nicotiana tabacum
2.3.1.20 diacylglycerol acyltransferase
-
 Rattus norvegicus
2.3.1.20 diacylglycerol acyltransferase
-
 Spinacia oleracea
2.3.1.20 diacylglycerol acyltransferase
-
 Zea mays
2.3.1.20 diacylglycerol acyltransferase
-
 Glycine max
2.3.1.20 diacylglycerol acyltransferase
-
 Acinetobacter calcoaceticus
2.3.1.20 diacylglycerol acyltransferase
-
 Arachis hypogaea
2.3.1.20 diacylglycerol acyltransferase
-
 Carthamus tinctorius
2.3.1.20 diacylglycerol acyltransferase
-
 Umbelopsis ramanniana
2.3.1.20 diacylglycerol acyltransferase
-
 Olea europaea
2.3.1.20 diacylglycerol acyltransferase
-
 Ricinus communis
2.3.1.20 diacylglycerol acyltransferase
-
 Mus musculus
2.3.1.20 diacylglycerol acyltransferase
-
 Brassica napus
2.3.1.20 diacylglycerol acyltransferase
-
 Cuphea sp.
2.3.1.20 diacylglycerol acyltransferase
-
 Arabidopsis thaliana
2.3.1.20 diacylglycerol acyltransferase
-
 Nicotiana tabacum