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Literature summary extracted from

  • Capendeguy, O.; Horisberger, J.D.
    The role of the third extracellular loop of the Na+,K+-ATPase alpha subunit in a luminal gating mechanism (2005), J. Physiol., 565, 207-218.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.2.2.13 in pSD5 vector, expression of proteins in Xenopus laevis oocytes Rhinella marina

Protein Variants

EC Number Protein Variants Comment Organism
7.2.2.13 C111S control Rhinella marina
7.2.2.13 G803C the effect of cysteine mutations on the K+ activated and ouabain-sensitive currents, the effect of acid pH and omeprazole, the effect of MTSET on Na+, K+-ATPase cysteine mutants and the effect of MTSET on the voltage-dependent kinetics of activation by extracellular K+ is measured. Rhinella marina
7.2.2.13 L802C the effect of cysteine mutations on the K+ activated and ouabain-sensitive currents, the effect of acid pH and omeprazole, the effect of MTSET on Na+, K+-ATPase cysteine mutants and the effect of MTSET on the voltage-dependent kinetics of activation by extracellular K+ is measured. Rhinella marina
7.2.2.13 P801C the effect of cysteine mutations on the K+ activated and ouabain-sensitive currents, the effect of acid pH and omeprazole, the effect of MTSET on Na+, K+-ATPase cysteine mutants and the effect of MTSET on the voltage-dependent kinetics of activation by extracellular K+ is measured. Rhinella marina
7.2.2.13 T804C the effect of cysteine mutations on the K+ activated and ouabain-sensitive currents, the effect of acid pH and omeprazole, the effect of MTSET on Na+, K+-ATPase cysteine mutants and the effect of MTSET on the voltage-dependent kinetics of activation by extracellular K+ is measured. Rhinella marina
7.2.2.13 V805C the effect of cysteine mutations on the K+ activated and ouabain-sensitive currents, the effect of acid pH and omeprazole, the effect of MTSET on Na+, K+-ATPase cysteine mutants and the effect of MTSET on the voltage-dependent kinetics of activation by extracellular K+ is measured. Rhinella marina

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.2.2.13 MTSET [2-(trimethylammonium)ethyl]methanethiosulphonate bromide, inhibits mutants G803C, V805C Rhinella marina
7.2.2.13 Omeprazole inhibit mutants G803C, T804C, V805C Rhinella marina
7.2.2.13 Ouabain g-strophanthin Rhinella marina

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.2.2.13 membrane
-
Rhinella marina 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.2.2.13 55000
-
glycosylated beta subunit Rhinella marina
7.2.2.13 110000
-
alpha subunit Rhinella marina

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.2.2.13 ADP + phosphate + Na+/out Rhinella marina
-
ATP + H2O + Na+/in
-
?
7.2.2.13 ATP + H2O + K+/in Rhinella marina
-
ADP + phosphate + K+/out
-
?

Organism

EC Number Organism UniProt Comment Textmining
7.2.2.13 Rhinella marina
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.2.13 ADP + phosphate + Na+/out
-
Rhinella marina ATP + H2O + Na+/in
-
?
7.2.2.13 ATP + H2O + K+/in
-
Rhinella marina ADP + phosphate + K+/out
-
?

Subunits

EC Number Subunits Comment Organism
7.2.2.13 dimer heterodimer, alpha and beta subunit Rhinella marina

Synonyms

EC Number Synonyms Comment Organism
7.2.2.13 Na+,K+-ATPase
-
Rhinella marina