EC Number | Cloned (Comment) | Organism |
---|---|---|
7.2.2.13 | in pSD5 vector, expression of proteins in Xenopus laevis oocytes | Rhinella marina |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
7.2.2.13 | C111S | control | Rhinella marina |
7.2.2.13 | G803C | the effect of cysteine mutations on the K+ activated and ouabain-sensitive currents, the effect of acid pH and omeprazole, the effect of MTSET on Na+, K+-ATPase cysteine mutants and the effect of MTSET on the voltage-dependent kinetics of activation by extracellular K+ is measured. | Rhinella marina |
7.2.2.13 | L802C | the effect of cysteine mutations on the K+ activated and ouabain-sensitive currents, the effect of acid pH and omeprazole, the effect of MTSET on Na+, K+-ATPase cysteine mutants and the effect of MTSET on the voltage-dependent kinetics of activation by extracellular K+ is measured. | Rhinella marina |
7.2.2.13 | P801C | the effect of cysteine mutations on the K+ activated and ouabain-sensitive currents, the effect of acid pH and omeprazole, the effect of MTSET on Na+, K+-ATPase cysteine mutants and the effect of MTSET on the voltage-dependent kinetics of activation by extracellular K+ is measured. | Rhinella marina |
7.2.2.13 | T804C | the effect of cysteine mutations on the K+ activated and ouabain-sensitive currents, the effect of acid pH and omeprazole, the effect of MTSET on Na+, K+-ATPase cysteine mutants and the effect of MTSET on the voltage-dependent kinetics of activation by extracellular K+ is measured. | Rhinella marina |
7.2.2.13 | V805C | the effect of cysteine mutations on the K+ activated and ouabain-sensitive currents, the effect of acid pH and omeprazole, the effect of MTSET on Na+, K+-ATPase cysteine mutants and the effect of MTSET on the voltage-dependent kinetics of activation by extracellular K+ is measured. | Rhinella marina |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
7.2.2.13 | MTSET | [2-(trimethylammonium)ethyl]methanethiosulphonate bromide, inhibits mutants G803C, V805C | Rhinella marina | |
7.2.2.13 | Omeprazole | inhibit mutants G803C, T804C, V805C | Rhinella marina | |
7.2.2.13 | Ouabain | g-strophanthin | Rhinella marina |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
7.2.2.13 | membrane | - |
Rhinella marina | 16020 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
7.2.2.13 | 55000 | - |
glycosylated beta subunit | Rhinella marina |
7.2.2.13 | 110000 | - |
alpha subunit | Rhinella marina |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.2.2.13 | ADP + phosphate + Na+/out | Rhinella marina | - |
ATP + H2O + Na+/in | - |
? | |
7.2.2.13 | ATP + H2O + K+/in | Rhinella marina | - |
ADP + phosphate + K+/out | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
7.2.2.13 | Rhinella marina | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.2.2.13 | ADP + phosphate + Na+/out | - |
Rhinella marina | ATP + H2O + Na+/in | - |
? | |
7.2.2.13 | ATP + H2O + K+/in | - |
Rhinella marina | ADP + phosphate + K+/out | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
7.2.2.13 | dimer | heterodimer, alpha and beta subunit | Rhinella marina |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
7.2.2.13 | Na+,K+-ATPase | - |
Rhinella marina |