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Literature summary extracted from

  • Kawata, M.; Kinoshita, K.; Takahashi, S.; Ogura, K.; Komoto, N.; Yamanishi, M.; Tobimatsu, T.; Toraya, T.
    Survey of Catalytic Residues and Essential Roles of Glutamate-aplpha170 and Aspartate-alpha335 in Coenzyme B12-dependent Diol Dehydratase (2006), J. Biol. Chem., 281, 18327-18334.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.28 as complexes with coenzyme analogs in the substrate-bound and substrate-free forms Klebsiella oxytoca

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.28 D335A inactive mutant Klebsiella oxytoca
4.2.1.28 D335A does not form (alpha-beta-gamma)complex Klebsiella oxytoca
4.2.1.28 D335N inactive mutant Klebsiella oxytoca
4.2.1.28 E170A inactive mutant Klebsiella oxytoca
4.2.1.28 E170H does not form (alpha-beta-gamma)complex Klebsiella oxytoca
4.2.1.28 E221A does not form (alpha-beta-gamma)complex Klebsiella oxytoca
4.2.1.28 H143A only very little activity Klebsiella oxytoca
4.2.1.28 Q141A partial activity Klebsiella oxytoca
4.2.1.28 Q296A increased Km for substrate(1,2-propanediol) by a factor of 250 Klebsiella oxytoca
4.2.1.28 S362A partial activity Klebsiella oxytoca

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.28 0.04
-
 1,2-propanediol
-
 Klebsiella oxytoca
4.2.1.28 0.08
-
 1,2-propanediol mutant S362A Klebsiella oxytoca
4.2.1.28 0.1
-
 1,2-propanediol mutant Q141A Klebsiella oxytoca
4.2.1.28 10
-
 1,2-propanediol mutant Q296A Klebsiella oxytoca

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.28 K+
-
 Klebsiella oxytoca

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.28 Klebsiella oxytoca
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.28
-
 Klebsiella oxytoca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.28 1,2-ethanediol
-
 Klebsiella oxytoca acetaldehyde + H2O
-
 ?
4.2.1.28 1,2-propanediol
-
 Klebsiella oxytoca propanal + H2O
-
 ?
4.2.1.28 glycerol
-
 Klebsiella oxytoca beta-hydroxypropionaldehyde + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.28 hexamer dimer of a heterotrimer Klebsiella oxytoca

Synonyms

EC Number Synonyms Comment Organism
4.2.1.28 diol dehydratase
-
 Klebsiella oxytoca

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.28 adenosylcobalamin
-
 Klebsiella oxytoca