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Literature summary extracted from

  • Abergel, C.; Rudinger-Thirion, J.; Giege, R.; Claverie, J.M.
    Virus-encoded aminoacyl-tRNA synthetases: structural and functional characterization of mimivirus TyrRS and MetRS (2007), J. Virol., 81, 12406-12417.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.1 TyrRS, phylogenetic analysis, the viral aminoacyl tRNA synthetases have not been acquired recently by horizontal gene transfer from a cellular host but rather militate in favor of an intricate evolutionary relationship between large DNA viruses and ancestral eukaryotes Acanthamoeba polyphaga Mimivirus
6.1.1.10 MetRS, phylogenetic analysis, the viral aminoacyl tRNA synthetases have not been acquired recently by horizontal gene transfer from a cellular host but rather militate in favor of an intricate evolutionary relationship between large DNA viruses and ancestral eukaryotes Acanthamoeba polyphaga Mimivirus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.1 purified selenomethionyl-TyrRSapm in complex with tyrosinol, crystallization is improved by introducing anion-exchange chromatography, vapour diffusion method, 0.002 ml of 14 mg/ml protein in 20 mM Tris-HCl, pH 7.4, 25°C, is mixed with 500 nl reservoir solution containing 0.1 M sodium citrate, pH 5.5, and 6-9% PEG 4000 w/v, 15% 2-methyl-2,4-pentane-d12-diol, 0.1 M KCl, 1 mM MgCl2, X-ray diffraction structure determination and analysis at 2.2 A resolution, molecular replacement Acanthamoeba polyphaga Mimivirus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.1 additional information
-
 additional information kinetics with Escherichia coli tRNATyr, Plasmodium falciparum tRNATyr, and diverse wild-type and mutant Saccharomyces cerevisiae tRNATyrs, overview Acanthamoeba polyphaga Mimivirus
6.1.1.10 additional information
-
 additional information kinetics with Escherichia coli native tRNAMet and initiator tRNAMet, and Saccharomyces cerevisiae native tRNAMet and initiator tRNAMet, overview Acanthamoeba polyphaga Mimivirus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.1 Mg2+
-
 Acanthamoeba polyphaga Mimivirus
6.1.1.10 Mg2+
-
 Acanthamoeba polyphaga Mimivirus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.1 ATP + L-tyrosine + tRNATyr Acanthamoeba polyphaga Mimivirus the TyrRS specificity for tyrosine and conformity with the identity rules for tRNATyr for archea/eukarya, anticodon binding site, overview AMP + diphosphate + L-tyrosyl-tRNATyr
-
 ?
6.1.1.1 additional information Acanthamoeba polyphaga Mimivirus mimivirus aminoacyl-tRNA synthetases function as regular translation enzymes in infected amoebas ?
-
 ?
6.1.1.10 ATP + L-methionine + tRNAMet Acanthamoeba polyphaga Mimivirus the MetRS specificity for methionine and conformity with the identity rules for tRNAMet for archea/eukarya, anticodon binding site, overview AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
6.1.1.10 additional information Acanthamoeba polyphaga Mimivirus mimivirus aminoacyl-tRNA synthetases function as regular translation enzymes in infected amoebas ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.1 Acanthamoeba polyphaga Mimivirus Q5UPJ7
-
-
6.1.1.10 Acanthamoeba polyphaga Mimivirus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.1 additional information
-
 activity with Escherichia coli tRNATyr, Plasmodium falciparum tRNATyr, and diverse wild-type and mutant Saccharomyces cerevisiae tRNATyrs, overview Acanthamoeba polyphaga Mimivirus
6.1.1.10 additional information
-
 activity with Escherichia coli native tRNAMet and initiator tRNAMet, and Saccharomyces cerevisiae native tRNAMet and initiator tRNAMet, overview Acanthamoeba polyphaga Mimivirus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.1 ATP + L-tyrosine + tRNATyr the TyrRS specificity for tyrosine and conformity with the identity rules for tRNATyr for archea/eukarya, anticodon binding site, overview Acanthamoeba polyphaga Mimivirus AMP + diphosphate + L-tyrosyl-tRNATyr
-
 ?
6.1.1.1 ATP + L-tyrosine + tRNATyr functional idiosyncrasies of the viral TyrRS, activity with Escherichia coli tRNATyr, Plasmodium falciparum tRNATyr, and diverse wild-type and mutant Saccharomyces cerevisiae tRNATyrs, overview, the TyrRS specificity for tyrosine and recognition of the tRNATyr acceptor stem show conformity with the identity rules for tRNATyr for archea/eukarya, anticodon binding site, overview Acanthamoeba polyphaga Mimivirus AMP + diphosphate + L-tyrosyl-tRNATyr
-
 ?
6.1.1.1 additional information mimivirus aminoacyl-tRNA synthetases function as regular translation enzymes in infected amoebas Acanthamoeba polyphaga Mimivirus ?
-
 ?
6.1.1.10 ATP + L-methionine + tRNAMet the MetRS specificity for methionine and conformity with the identity rules for tRNAMet for archea/eukarya, anticodon binding site, overview Acanthamoeba polyphaga Mimivirus AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
6.1.1.10 ATP + L-methionine + tRNAMet functional idiosyncrasies of the viral MetRS, activity with Escherichia coli native tRNAMet and initiator tRNAMet, and Saccharomyces cerevisiae native tRNAMet and initiator tRNAMet, overview, the MetRS specificity for methionine and recognition of the tRNAMet acceptor stem show conformity with the identity rules for tRNAMet for archea/eukarya, overview Acanthamoeba polyphaga Mimivirus AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
6.1.1.10 additional information mimivirus aminoacyl-tRNA synthetases function as regular translation enzymes in infected amoebas Acanthamoeba polyphaga Mimivirus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.1 dimer dimer interface structure from crystal structure analysis, modeling, overview Acanthamoeba polyphaga Mimivirus

Synonyms

EC Number Synonyms Comment Organism
6.1.1.1 Tyrosyl-tRNA synthetase
-
 Acanthamoeba polyphaga Mimivirus
6.1.1.1 TyrRS
-
 Acanthamoeba polyphaga Mimivirus
6.1.1.10 methionyl-tRNA synthetase
-
 Acanthamoeba polyphaga Mimivirus
6.1.1.10 MetRS
-
 Acanthamoeba polyphaga Mimivirus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.1 30
-
 assay at Acanthamoeba polyphaga Mimivirus
6.1.1.10 30
-
 assay at Acanthamoeba polyphaga Mimivirus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.1 7.5
-
 assay at Acanthamoeba polyphaga Mimivirus
6.1.1.10 7.5
-
 assay at Acanthamoeba polyphaga Mimivirus

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.1 ATP
-
 Acanthamoeba polyphaga Mimivirus
6.1.1.10 ATP
-
 Acanthamoeba polyphaga Mimivirus