Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rosati, F.; Bardazzi, I.; De Blasi, P.; Simi, L.; Scarpi, D.; Guarna, A.; Serio, M.; Racchi, M.L.; Danza, G.
    5alpha-Reductase activity in Lycopersicon esculentum: cloning and functional characterization of LeDET2 and evidence of the presence of two isoenzymes (2005), J. Steroid Biochem. Mol. Biol., 96, 287-299.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.3.1.22 additional information two isoenzymes of 5alphaR is probably characteristic of the whole plant kingdom Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.22 into vector pTarget and expressed in COS-7 cells Arabidopsis thaliana
1.3.1.22 into vector pTarget and expressed in COS-7 cells Solanum lycopersicum

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.22 K204E det2-1 mutant, substitution of lysine for Glu-204 totally inactivates the steroid 5alpha-reductase activity, residual activity for campestenone and progesterone Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.22 4-MA active at 0.01 mM Solanum lycopersicum
1.3.1.22 AFA131 active at 0.01 mM Solanum lycopersicum
1.3.1.22 AFA192 active at 0.01 mM Solanum lycopersicum
1.3.1.22 AFA27 active at 0.01 mM Solanum lycopersicum
1.3.1.22 AFA76 active at 0.01 mM, most potent inhibitor among non-steroid compounds tested Solanum lycopersicum
1.3.1.22 AS601811 active at 0.01 mM Solanum lycopersicum
1.3.1.22 finasteride active at 0.01 mM Solanum lycopersicum
1.3.1.22 PD17 active at 0.01 mM Solanum lycopersicum
1.3.1.22 PD91 active at 0.01 mM Solanum lycopersicum
1.3.1.22 VG106 active at 0.01 mM Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.22 0.00075
-
 progesterone
-
 Solanum lycopersicum
1.3.1.22 0.02634
-
 androstenedione
-
 Solanum lycopersicum
1.3.1.22 0.0446
-
 testosterone
-
 Solanum lycopersicum

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.22 Arabidopsis thaliana
-
-
-
1.3.1.22 Solanum lycopersicum Q5K2N1
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.22 callus
-
 Solanum lycopersicum
-
1.3.1.22 leaf
-
 Arabidopsis thaliana
-
1.3.1.22 leaf higher levels Solanum lycopersicum
-
1.3.1.22 root
-
 Solanum lycopersicum
-
1.3.1.22 seed
-
 Arabidopsis thaliana
-
1.3.1.22 seed
-
 Solanum lycopersicum
-
1.3.1.22 stem
-
 Solanum lycopersicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.22 androstenedione + NADPH + H+
-
 Solanum lycopersicum 5alpha-androstan-3,17-dione + NADP+
-
 ?
1.3.1.22 campestenone + NADPH reduction at very low levels Solanum lycopersicum 5alpha-campestan-3-one + NADP+
-
 ?
1.3.1.22 additional information no activity for campestenone Arabidopsis thaliana ?
-
 ?
1.3.1.22 progesterone + NADPH + H+
-
 Arabidopsis thaliana 5alpha-pregnan-3-20-dione + NADP+
-
 ?
1.3.1.22 progesterone + NADPH + H+
-
 Solanum lycopersicum 5alpha-pregnan-3-20-dione + NADP+
-
 ?
1.3.1.22 testosterone + NADPH + H+
-
 Solanum lycopersicum 5alpha-dihydrotestosterone + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.22 5alphaR
-
 Arabidopsis thaliana
1.3.1.22 5alphaR
-
 Solanum lycopersicum
1.3.1.22 AtDET2
-
 Arabidopsis thaliana
1.3.1.22 LeDET2
-
 Solanum lycopersicum
1.3.1.22 steroid 5alpha-reductase
-
 Arabidopsis thaliana
1.3.1.22 steroid 5alpha-reductase
-
 Solanum lycopersicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.22 NADPH
-
 Solanum lycopersicum