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Literature summary extracted from

  • Sharma, R.; Soll, J.; Boelter, B.
    Import and localisation of nucleoside diphosphate kinase 2 in chloroplasts (2007), J. Plant Res., 120, 451-456.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.6 transient expression of GFP-tagged wild-type and mutant NDPK2s in Pisum sativum protoplasts Pisum sativum

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.6 A77T site-directed mutagenesis, the mutant shows an altered import behaviour into chloroplasts compared to the wild-type enzyme, overview Pisum sativum
2.7.4.6 R63D site-directed mutagenesis, the mutant shows an altered import behaviour into chloroplasts compared to the wild-type enzyme, overview Pisum sativum
2.7.4.6 R74D site-directed mutagenesis, the mutant shows an altered import behaviour into chloroplasts compared to the wild-type enzyme, overview Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.6 chloroplast chloroplast stroma Pisum sativum 9507
-
2.7.4.6 chloroplast stroma NDPK2 contains a predicted chloroplast targeting transit peptide of 63 amino acids, import mechanism, overview Pisum sativum 9570
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.6 17400
-
 x * 25000, precursor protein, x * 18500, and x * 17400, mature enzyme forms Pisum sativum
2.7.4.6 18500
-
 x * 25000, precursor protein, x * 18500, and x * 17400, mature enzyme forms Pisum sativum
2.7.4.6 18500
-
 x * 18500, mature form, SDS-PAGE, x * 25000, precursor form, SDS-PAGE Pisum sativum
2.7.4.6 25000
-
 x * 25000, precursor protein, x * 18500, and x * 17400, mature enzyme forms Pisum sativum
2.7.4.6 25000
-
 x * 18500, mature form, SDS-PAGE, x * 25000, precursor form, SDS-PAGE Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.6 additional information Pisum sativum key enzyme involved in the homeostasis of nucleoside triphosphates ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.6 Pisum sativum
-
 two mature isoforms of NDPK2 in plastids
-
2.7.4.6 Pisum sativum P47923
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.4.6 proteolytic modification 25000 Da, precursor protein, 18500 Da and 17400 Da, mature enzyme forms starting at amino acids 65 and 78 resp. Pisum sativum

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.6 native enzyme from chloroplasts to homogeneity, from cytosol and membranes Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.6 leaf
-
 Pisum sativum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.6 additional information key enzyme involved in the homeostasis of nucleoside triphosphates Pisum sativum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.6 ? x * 25000, precursor protein, x * 18500, and x * 17400, mature enzyme forms Pisum sativum
2.7.4.6 ? x * 18500, mature form, SDS-PAGE, x * 25000, precursor form, SDS-PAGE Pisum sativum

Synonyms

EC Number Synonyms Comment Organism
2.7.4.6 NDPK2
-
 Pisum sativum
2.7.4.6 nucleoside diphosphate kinase 2
-
 Pisum sativum