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Literature summary extracted from

  • Xu, B.B.; Li, J.N.; Zhang, X.K.; Wang, R.; Xie, L.L.; Chai, Y.R.
    Cloning and molecular characterization of a functional flavonoid 3'-hydroxylase gene from Brassica napus (2007), J. Plant Physiol., 164, 350-363.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.82 gene F3'H-1, two clones, DNA and amio acid sequence determination and analysis, sequence comparison, expression analysis, alternative polyadenylation in the 3'-UTR is adopted by this gene to generate heterogeneous transcripts, phylogenetic tree, functional expression of His-tagged F3'H-1 in Escherichia coli Brassica napus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.82 56620
-
 x * 56620, amino acid sequence calculation Brassica napus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.82 dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2 Brassica napus
-
 dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 additional information Brassica napus transcription level of the F3'H genes is not the reason for the different seed colorations found in near-isogenic lines, blackseeded L1 and yellow-seeded L2, of Brassica napus ?
-
 ?
1.14.14.82 naringenin + [reduced NADPH-hemoprotein reductase] + O2 Brassica napus
-
 eriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.82 Brassica napus A1XBC6 gene F3'H-1, two clones
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.82 additional information F3'H-1 tissue expression analysis Brassica napus
-
1.14.14.82 pericarp
-
 Brassica napus
-
1.14.14.82 seed
-
 Brassica napus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.82 dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
 Brassica napus dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 additional information transcription level of the F3'H genes is not the reason for the different seed colorations found in near-isogenic lines, blackseeded L1 and yellow-seeded L2, of Brassica napus Brassica napus ?
-
 ?
1.14.14.82 naringenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Brassica napus eriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.82 ? x * 56620, amino acid sequence calculation Brassica napus
1.14.14.82 More the secondary structure is dominated by alpha-helices and random coils Brassica napus

Synonyms

EC Number Synonyms Comment Organism
1.14.14.82 F3'H
-
 Brassica napus
1.14.14.82 flavonoid 3'-hydroxylase
-
 Brassica napus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.14.82 Brassica napus amino acid sequence calculation
-
 7.1