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Literature summary extracted from

  • Lamba, J.K.; Crews, K.; Pounds, S.; Schuetz, E.; Gresham, J.; Gandhi, V.; Plunkett, W.; Rubnitz, J.; Ribeiro, R.
    Pharmacogenetics of deoxycytidine kinase: Identification and characterization of novel genetic variants (2007), J. Pharmacol. Exp. Ther., 323, 935-945.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.1.74 medicine sequencing of gene from European and African individuals reveals 64 genetic polymorphisms. In general, African ancestry subjects show higher mRNA expression compared with subjects with European ancestry. In both groups, single nucleotide polymorphism 35708 C>T of a 3'-untranslated region is significantly associated with lower mRNA expression and with lower blast 1-beta-D-arabinofuranosylcytosine 5'-triphosphate levels in acute myeloid leukemia patients Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.74 native and mutant proteins expressed in Escherichia coli BL21 star Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.74 A119G naturally occurring mutation, 66% of activity of the wild type protein Homo sapiens
2.7.1.74 A119G genetic polymorphism, 66% of wild-type activity, decrease in Km value Homo sapiens
2.7.1.74 I24V naturally occurring mutation, 85% of activity of the wild type protein Homo sapiens
2.7.1.74 I24V genetic polymorphism, 85% of wild-type activity Homo sapiens
2.7.1.74 additional information sequencing of gene from European and African individuals reveals 64 genetic polymorphisms. In general, African ancestry subjects show higher mRNA expression compared with subjects with European ancestry. In both groups, single nucleotide polymorphism 35708 C>T of a 3'-untranslated region is significanlty associated with lower mRNA expression and with lower blast 1-beta-D-arabinofuranosylcytosine 5'-triphosphate levels in acute myeloid leukemia patients Homo sapiens
2.7.1.74 P122S naturally occurring mutation, 43% of activity of the wild type protein Homo sapiens
2.7.1.74 P122S genetic polymorphism, 43% of wild-type activity, decrease in Km value Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.74 0.004
-
2-chloro-2'-deoxyadenosine A119G mutant enzyme, 37°C, pH 7.4 Homo sapiens
2.7.1.74 0.004
-
2-chloro-2'-deoxyadenosine P122S mutant enzyme, 37°C, pH 7.4 Homo sapiens
2.7.1.74 0.006
-
2-chloro-2'-deoxyadenosine wild type enzyme, 37°C, pH 7.4 Homo sapiens
2.7.1.74 0.009
-
2-chloro-2'-deoxyadenosine I24V mutant enzyme, 37°C, pH 7.4 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.74 Mg2+ in complex with ATP Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.74 NTP + 1-beta-D-arabinofuranosylcytosine Homo sapiens i.e. cytarabine, rate limiting enzyme in the activation of the prodrug NDP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.74 Homo sapiens
-
differences in mRNA levels in African and European individuals detected
-
2.7.1.74 Homo sapiens P27707
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.74 B-lymphoblastoid cell line
-
Homo sapiens
-
2.7.1.74 lymphoblast
-
Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.74 ATP + 2-chloro-2'-deoxyadenosine i.e. cladribine Homo sapiens ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
?
2.7.1.74 NTP + 1-beta-D-arabinofuranosylcytosine i.e. cytarabine, rate limiting enzyme in the activation of the prodrug Homo sapiens NDP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.74 dCK
-
Homo sapiens