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Literature summary extracted from

  • Ji, Y.; Moon, I.; Zlatkovic, J.; Salavaggione, O.E.; Thomae, B.A.; Eckloff, B.W.; Wieben, E.D.; Schaid, D.J.; Weinshilboum, R.M.
    Human hydroxysteroid sulfotransferase SULT2B1 pharmacogenomics: gene sequence variation and functional genomics (2007), J. Pharmacol. Exp. Ther., 322, 529-540.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.2 expression of GFP-tagged wild-type allozymes and mutant truncated SULT2B1a and SULT2B1b variants lacking the SULT2B1 proline-rich tail in COS-1 cells Homo sapiens
2.8.2.2 isozymes SULT2B1a and SULT2B1b are encoded by a single SULT2B1 gene as a result of alternative transcription initiation and alternative splicing, genotyping of African-American and Caucasian-American individuals, transient expression of GFP-tagged SULT2B1a and SULT2B1b in COS-1 cells, in vitro transcription and translation of truncated SULT2B1a and SULT2B1b variants that lack the SULT2B1 proline-rich tail using rabbit reticulocyte lysate Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.8.2.2 D176N naturally occuring polymorphism in SULT2B1a Homo sapiens
2.8.2.2 L36S naturally occuring polymorphism in SULT2B1a Homo sapiens
2.8.2.2 additional information construction of truncated SULT2B1a and SULT2B1b variants that lack the SULT2B1 proline-rich tail, genotype-to-phenotype approach to identify and characterize common sequence variation in SULT2B1, determination and analysis of 100 polymorphisms in African-American and Caucasian-American individuals, including four nonsynonymous coding single nucleotide polymorphisms and one 6-base pair deletion, within the shared region of the open reading frame, overview Homo sapiens
2.8.2.2 additional information construction of truncated SULT2b1a and SULT2B1b variants by site-directed mutagenesis to introduce a translation termination codon immediately before the final 53 amino acids at the C terminus of the protein. Pharmacogenomics, gene sequence variation and functional genomics, determination of 100 polymorphisms, including four nonsynonymous coding single nucleotide polymorphisms and one 6-base pair deletion, in African-American and Caucasian-American individuals, overview Homo sapiens
2.8.2.2 P330L naturally occuring polymorphism in SULT2B1a Homo sapiens
2.8.2.2 R215H naturally occuring polymorphism in SULT2B1a Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.2 cytosol
-
 Homo sapiens 5829
-
2.8.2.2 additional information subcellular localization of isozymes SULT2B1a and SULT2B1b, overview Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.2 Mg2+
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.2 Homo sapiens
-
 isozymes SULT2B1a and SULT2B1b
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.2 MCF-7 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.2 3'-phosphoadenylyl sulfate + cholesterol
-
 Homo sapiens adenosine 3',5'-bisphosphate + cholesterol 3-sulfate
-
 ?
2.8.2.2 3'-phosphoadenylyl sulfate + cholesterol preferred substrate of SULT2B1b Homo sapiens adenosine 3',5'-bisphosphate + cholesterol 3-sulfate
-
 ?
2.8.2.2 3'-phosphoadenylyl sulfate + dehydroepiandrosterone
-
 Homo sapiens adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
 ?
2.8.2.2 3'-phosphoadenylyl sulfate + dehydroepiandrosterone preferred substrate of SULT2B1b Homo sapiens adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
 ?
2.8.2.2 3'-phosphoadenylyl sulfate + pregnenolone
-
 Homo sapiens adenosine 3',5'-bisphosphate + pregnenolone 3-sulfate
-
 ?
2.8.2.2 3'-phosphoadenylyl sulfate + pregnenolone preferred substrate of SULT2B1a Homo sapiens adenosine 3',5'-bisphosphate + pregnenolone 3-sulfate
-
 ?
2.8.2.2 additional information SULT2B1b catalyzes the sulfonation of 3beta-hydroxysteroid hormones and cholesterol, whereas SULT2B1a preferentially catalyzes pregnenolone sulfonation Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.8.2.2 hydroxysteroid sulfotransferase
-
 Homo sapiens
2.8.2.2 More SULT2B1 is a member of the cytosolic SULT gene family Homo sapiens
2.8.2.2 SULT2B1
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.2 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.2 5.5
-
 assay at Homo sapiens
2.8.2.2 5.5 6.5 assay at Homo sapiens