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Literature summary extracted from
- Identification and functional analysis of common human flavin-containing monooxygenase 3 genetic variants (2007), J. Pharmacol. Exp. Ther., 320, 266-273.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.13.8 | DNA and amino acid sequence determination and analysis of multiple samples of gene FMO3, expression of clones in Spodoptera frugiperda Sf9 insect cell microsomes via baculovirus transfection system, the FMO multigene family consists of a five-gene cluster at 1q24.3, comprising FMO1-4 and FMO6p, and a second cluster of five genes at 1q24.2, comprising FMO7p-11p, and a single gene, FMO5, at 1q21.1, encoding a total of five active proteins in humans | Homo sapiens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.14.13.8 | E158K | naturally occuring polymorphism of FMO3, frequency in different human populations, the mutation has an impact on protein structure, overview | Homo sapiens |
| 1.14.13.8 | E24D | naturally occuring polymorphism of FMO3, frequency in different human populations, the mutation has an impact on protein structure, overview, the mutant shows altered substrate specificity compared to the wild-type mutant | Homo sapiens |
| 1.14.13.8 | E308G | naturally occuring polymorphism of FMO3, frequency in different human populations, the mutation has an impact on protein structure, overview | Homo sapiens |
| 1.14.13.8 | K416N | naturally occuring polymorphism of FMO3, frequency in different human populations, the mutation has an impact on protein structure, overview, the mutant shows altered substrate specificity compared to the wild-type mutant | Homo sapiens |
| 1.14.13.8 | additional information | determination and analysis of frequencies of 18 FMO3 single-nucleotide polymorphisms in 202 Hispanics of Mexican descent, 201 African Americans, and 200 non-Latino whites, synonymous mutations, and hypomorphic haplotypes, overview | Homo sapiens |
| 1.14.13.8 | N61K | naturally occuring polymorphism of FMO3, frequency in different human populations, the mutation has an impact on protein structure, overview, the mutant shows altered substrate specificity compared to the wild-type mutant | Homo sapiens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.8 | additional information | - |
additional information | kinetics of wild-type and mutant structural variants | Homo sapiens | |
| 1.14.13.8 | 0.0209 | - |
trimethylamine | recombinant wild-type enzyme, pH 8.5 | Homo sapiens |  |
| 1.14.13.8 | 0.0228 | - |
trimethylamine | recombinant mutant E24D, pH 8.5 | Homo sapiens | |
| 1.14.13.8 | 0.0373 | - |
trimethylamine | recombinant mutant K416N, pH 8.5 | Homo sapiens | |
| 1.14.13.8 | 0.0502 | - |
ethylenethiourea | recombinant mutant K416N, pH 8.5 | Homo sapiens | |
| 1.14.13.8 | 0.0557 | - |
ethylenethiourea | recombinant wild-type enzyme, pH 8.5 | Homo sapiens | |
| 1.14.13.8 | 0.0665 | - |
sulindac | recombinant mutant K416N, pH 8.5 | Homo sapiens | |
| 1.14.13.8 | 0.0718 | - |
Methimazole | recombinant wild-type enzyme, pH 8.5 | Homo sapiens | |
| 1.14.13.8 | 0.0761 | - |
ethylenethiourea | recombinant mutant E24D, pH 8.5 | Homo sapiens | |
| 1.14.13.8 | 0.0773 | - |
Methimazole | recombinant mutant K416N, pH 8.5 | Homo sapiens | |
| 1.14.13.8 | 0.0854 | - |
Methimazole | recombinant mutant E24D, pH 8.5 | Homo sapiens | |
| 1.14.13.8 | 0.1208 | - |
sulindac | recombinant mutant E24D, pH 8.5 | Homo sapiens | |
| 1.14.13.8 | 0.1233 | - |
Methimazole | recombinant mutant N61K, pH 8.5 | Homo sapiens | |
| 1.14.13.8 | 0.1501 | - |
sulindac | recombinant wild-type enzyme, pH 8.5 | Homo sapiens | |
| 1.14.13.8 | 0.2071 | - |
sulindac | recombinant mutant N61K, pH 8.5 | Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.13.8 | microsome | - |
Homo sapiens | - |
- |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.8 | additional information | Homo sapiens | the enzyme catalyzes the NADPH-dependent N-and S-oxidation of a variety of therapeutics, environmental toxicants, carcinogens, and nutrients | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.8 | Homo sapiens | - |
isozyme FMO3 | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.8 | additional information | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.8 | ethylenethiourea + NADPH + O2 | - |
Homo sapiens | ethylenethiourea S-oxide + NADP+ + H2O | - |
? | |
| 1.14.13.8 | methimazole + NADPH + O2 | - |
Homo sapiens | ? | - |
? | |
| 1.14.13.8 | additional information | the enzyme catalyzes the NADPH-dependent N-and S-oxidation of a variety of therapeutics, environmental toxicants, carcinogens, and nutrients | Homo sapiens | ? | - |
? | |
| 1.14.13.8 | sulindac sulfide + NADPH + O2 | - |
Homo sapiens | (S,R)-sulindac + NADP+ + H2O | - |
? | |
| 1.14.13.8 | trimethylamine + NADPH + O2 | - |
Homo sapiens | trimethylamine N-oxide + NADP+ + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.13.8 | FMO | - |
Homo sapiens |
| 1.14.13.8 | More | the enzyme belongs to the FMO multigene family | Homo sapiens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.8 | 0.003 | - |
sulindac | recombinant mutant N61K, pH 8.5 | Homo sapiens | |
| 1.14.13.8 | 0.004 | - |
Methimazole | recombinant mutant N61K, pH 8.5 | Homo sapiens | |
| 1.14.13.8 | 0.034 | - |
trimethylamine | recombinant wild-type enzyme, pH 8.5 | Homo sapiens | |
| 1.14.13.8 | 0.036 | - |
ethylenethiourea | recombinant wild-type enzyme, pH 8.5 | Homo sapiens | |
| 1.14.13.8 | 0.042 | - |
ethylenethiourea | recombinant mutant K416N, pH 8.5 | Homo sapiens | |
| 1.14.13.8 | 0.044 | - |
sulindac | recombinant mutant E24D, pH 8.5 | Homo sapiens | |
| 1.14.13.8 | 0.047 | - |
Methimazole | recombinant mutant K416N, pH 8.5 | Homo sapiens | |
| 1.14.13.8 | 0.047 | - |
sulindac | recombinant wild-type enzyme, pH 8.5 | Homo sapiens | |
| 1.14.13.8 | 0.05 | - |
ethylenethiourea | recombinant mutant E24D, pH 8.5 | Homo sapiens | |
| 1.14.13.8 | 0.051 | - |
trimethylamine | recombinant mutant K416N, pH 8.5 | Homo sapiens | |
| 1.14.13.8 | 0.065 | - |
trimethylamine | recombinant mutant E24D, pH 8.5 | Homo sapiens | |
| 1.14.13.8 | 0.068 | - |
sulindac | recombinant mutant K416N, pH 8.5 | Homo sapiens | |
| 1.14.13.8 | 0.124 | - |
Methimazole | recombinant wild-type enzyme, pH 8.5 | Homo sapiens | |
| 1.14.13.8 | 0.22 | - |
Methimazole | recombinant mutant E24D, pH 8.5 | Homo sapiens | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.8 | 8.5 | - |
assay at | Homo sapiens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.8 | FAD | - |
Homo sapiens | |
| 1.14.13.8 | NADPH | - |
Homo sapiens | |
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