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Literature summary extracted from

  • Walraven, J.M.; Doll, M.A.; Hein, D.W.
    Identification and characterization of functional rat arylamine N-acetyltransferase 3: comparisons with rat arylamine N-acetyltransferases 1 and 2 (2006), J. Pharmacol. Exp. Ther., 319, 369-375.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.5 gene nat3, DNA and amino acid sequence determination and analysis, functional expression of genes nat1, nat2, and nat3 in Escherichia coli strain JM105 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.5 0.0058
-
 4-aminobiphenyl recombinant isozyme Nat1 Rattus norvegicus
2.3.1.5 0.018
-
 4-aminobiphenyl recombinant isozyme Nat3 Rattus norvegicus
2.3.1.5 0.033
-
 2-Aminofluorene recombinant isozyme Nat3 Rattus norvegicus
2.3.1.5 0.037
-
 2-Aminofluorene recombinant isozyme Nat1 Rattus norvegicus
2.3.1.5 0.058
-
 N-Hydroxy-4-aminobiphenyl recombinant isozyme Nat1 Rattus norvegicus
2.3.1.5 0.062
-
 N-Hydroxy-4-aminobiphenyl recombinant isozyme Nat3 Rattus norvegicus
2.3.1.5 0.568
-
 2-Aminofluorene recombinant isozyme Nat2 Rattus norvegicus
2.3.1.5 1.57
-
 4-aminobiphenyl recombinant isozyme Nat2 Rattus norvegicus
2.3.1.5 3.3
-
 N-Hydroxy-4-aminobiphenyl recombinant isozyme Nat2 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.5 33500
-
 x * 33500, recombinant isozyme Nat3, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.5 acetyl-CoA + an arylamine Rattus norvegicus
-
 CoA + an N-acetylarylamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.5 Rattus norvegicus Q80SX3 isozyme Nat3; inbred strains F344, WKY, and SPRD, isozymes Nat1, Nat2, and Nat3
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.5 additional information
-
 substrate specificities of isozymes Nat1-Nat3 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.5 acetyl-CoA + 2-aminofluorene
-
 Rattus norvegicus CoA + 2-acetylaminofluorene
-
 ?
2.3.1.5 acetyl-CoA + 3,5-dimethylaniline
-
 Rattus norvegicus CoA + N-acetyl-3,5-dimethylaniline
-
 ?
2.3.1.5 acetyl-CoA + 3-ethylaniline
-
 Rattus norvegicus CoA + N-acetyl-3-ethylaniline
-
 ?
2.3.1.5 acetyl-CoA + 4,4'-methylenebis(2-chloroaniline)
-
 Rattus norvegicus CoA + N-acetyl-4,4'-methylenebis(2-chloroaniline)
-
 ?
2.3.1.5 acetyl-CoA + 4,4'-methylenedianiline
-
 Rattus norvegicus CoA + N-acetyl-4,4'-methylenedianiline
-
 ?
2.3.1.5 acetyl-CoA + 4-aminobiphenyl
-
 Rattus norvegicus CoA + 4-acetylaminobiphenyl
-
 ?
2.3.1.5 acetyl-CoA + 5-aminosalicylic acid
-
 Rattus norvegicus CoA + 5-acetylaminosalicylic acid
-
 ?
2.3.1.5 acetyl-CoA + an arylamine
-
 Rattus norvegicus CoA + an N-acetylarylamine
-
 ?
2.3.1.5 acetyl-CoA + N-hydroxy-4-aminobiphenyl O-acetylation activity Rattus norvegicus N-hydroxy-O-acetyl-4-aminobiphenyl + CoA
-
 ?
2.3.1.5 additional information substrate specificities of isozymes Nat1-Nat3, overview Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.5 ? x * 33500, recombinant isozyme Nat3, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.5 NAT
-
 Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.5 50
-
 recombinant isozyme Nat1 is completely stable for 120 min, recombinant isozyme Nat3 loses most activity within 80 min, recombinant isozyme Nat2 is almost inactivated within 5 min Rattus norvegicus