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Literature summary extracted from

  • Fox, D.T.; Poulter, C.D.
    Synthesis and evaluation of 1-deoxy-D-xylulose 5-phosphoric acid analogues as alternate substrates for methylerythritol phosphate synthase (2005), J. Org. Chem., 70, 1978-1985.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.267 1,1,1-trifluoro-1-deoxy-D-xylulose 5-phosphoric acid i.e. CF3-DXP, very low inhibition Escherichia coli
1.1.1.267 1,1-difluoro-1-deoxy-D-xylulose 5-phosphoric acid i.e. CF2-DXP, very low inhibition Escherichia coli
1.1.1.267 1,2-dideoxy-D-hexulose 6-phosphate i.e. Et-DXP, very low inhibition Escherichia coli
1.1.1.267 fosmidomycin
-
 Escherichia coli
1.1.1.267 FR900098 specific inhibition Escherichia coli
1.1.1.267 additional information inhibitor design and synthesis, overview Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.267 0.0005
-
 NADPH pH 7.6, 37°C, recombinant enzyme Escherichia coli
1.1.1.267 0.05
-
 1-deoxy-D-xylulose 5-phosphate pH 7.6, 37°C, recombinant enzyme Escherichia coli
1.1.1.267 0.227
-
 1-fluoro-1-deoxy-D-xylulose 5-phosphate pH 7.6, 37°C, recombinant enzyme Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ Escherichia coli
-
 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.267 Escherichia coli
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.267 2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ reaction mechanism Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+
-
 Escherichia coli 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 r
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ i.e. DXP Escherichia coli 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 r
1.1.1.267 1-fluoro-1-deoxy-D-xylulose 5-phosphate + NADPH i.e. CF-DXP Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.267 MEP synthase
-
 Escherichia coli
1.1.1.267 methylerythritol phosphate synthase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.267 37
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.267 29
-
 1-deoxy-D-xylulose 5-phosphate pH 7.6, 37°C, recombinant enzyme Escherichia coli
1.1.1.267 38
-
 1-deoxy-D-xylulose 5-phosphate pH 7.6, 37°C, recombinant enzyme Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.267 7.6
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.267 NADPH
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.267 2
-
 1,1,1-trifluoro-1-deoxy-D-xylulose 5-phosphoric acid pH 7.6, 37°C, recombinant enzyme Escherichia coli
1.1.1.267 3.4
-
 1,1-difluoro-1-deoxy-D-xylulose 5-phosphoric acid pH 7.6, 37°C, recombinant enzyme Escherichia coli
1.1.1.267 6.2
-
 1,2-dideoxy-D-hexulose 6-phosphate pH 7.6, 37°C, recombinant enzyme Escherichia coli