Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sakai, T.; Yamasaki, A.; Toyofuku, S.; Nishiki, T.; Yunoki, M.; Komoto, N.; Tobimatsu, T.; Toraya, T.
    Construction and characterization of hybrid dehydratases between adenosylcobalamin-dependent diol and glycerol dehydratases (2007), J. Nutr. Sci. Vitaminol., 53, 102-108.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.28 expression in E.coli Klebsiella oxytoca
4.2.1.28 expression in Escherichia coli Klebsiella pneumoniae
4.2.1.30 expression in Escherichia coli Klebsiella pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.30 glycerol suicide inactivation Klebsiella pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.28 0.04
-
 1,2-propanediol
-
 Klebsiella pneumoniae
4.2.1.28 0.04
-
 1,2-propanediol
-
 Klebsiella oxytoca
4.2.1.30 0.32
-
 1,2-propanediol
-
 Klebsiella pneumoniae
4.2.1.30 1.1
-
 glycerol
-
 Klebsiella pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.28 K+
-
 Klebsiella pneumoniae
4.2.1.28 K+
-
 Klebsiella oxytoca
4.2.1.28 Rb+ comparable activity to K+ Klebsiella pneumoniae
4.2.1.28 Rb+ comparable activity to K+ Klebsiella oxytoca
4.2.1.30 K+ bound in the inner part of the triose phosphate isomereas barrel of the alpha-subunit Klebsiella pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.28 Klebsiella oxytoca
-
-
-
4.2.1.28 Klebsiella pneumoniae
-
-
-
4.2.1.30 Klebsiella pneumoniae
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.30 120
-
-
 Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.28 1,2-propanediol
-
 Klebsiella pneumoniae propanal + H2O
-
 ?
4.2.1.28 1,2-propanediol
-
 Klebsiella oxytoca propanal + H2O
-
 ?
4.2.1.30 1,2-propanediol
-
 Klebsiella pneumoniae propionaldehyde + H2O
-
 ?
4.2.1.30 glycerol
-
 Klebsiella pneumoniae 3-hydroxypropionaldehyde + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.30 trimer (alpha/beta/gamma)2-structure, alpha 60000-61000 Da, beta 21000-24000 Da, gamma 16000-19000 Da Klebsiella pneumoniae

Synonyms

EC Number Synonyms Comment Organism
4.2.1.28 diol dehydratase
-
 Klebsiella pneumoniae
4.2.1.28 diol dehydratase
-
 Klebsiella oxytoca
4.2.1.30 glycerol dehydratase
-
 Klebsiella pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.28 adenosylcobalamin
-
 Klebsiella pneumoniae
4.2.1.28 adenosylcobalamin
-
 Klebsiella oxytoca
4.2.1.30 coenzyme B12
-
 Klebsiella pneumoniae