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Literature summary extracted from

  • Winge, I.; McKinney, J.A.; Knappskog, P.M.; Haavik, J.
    Characterization of wild-type and mutant forms of human tryptophan hydroxylase 2 (2007), J. Neurochem., 100, 1648-1657.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.16.4 DTT
-
 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.16.4 expression as maltose-binding protein fusion protein in Escherichia coli Homo sapiens
1.14.16.4 gene tph2, expression of TPH2 wild-type and mutants in Escherichia coli and in HEK-293 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.14.16.4 additional information effects of TPH2 mutations on their heterologous expression levels, solubility, thermal stability, secondary structure, and catalytic properties, overview Homo sapiens
1.14.16.4 P449R reduced thermal stability compared to the wild-type enzyme Homo sapiens
1.14.16.4 P449R site-directed mutagenesis, the TPH2 genetic variant, shows comparable catalytic activity (Vmax) and solubility relative to the wild type, but has decreased thermal stability Homo sapiens
1.14.16.4 R441H reduced solubility compared to the wild-type enzyme and reduced thermal stability. The mutant shows a 2fold lower Km for (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin Homo sapiens
1.14.16.4 R441H site-directed mutagenesis, the TPH2 genetic variant, shows had decreased activity, solubility and stability compared to the wild-type enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.16.4 dopamine 50% inhibition at 32 microM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.16.4 additional information
-
 additional information isozymes TPH1 and TPH2 kinetics, overview Homo sapiens
1.14.16.4 0.0147
-
 tetrahydrobiopterin pH 7.0, 30°C, recombinant mutant P449R fusion enzyme Homo sapiens
1.14.16.4 0.0186
-
 L-tryptophan pH 7.0, 30°C, recombinant mutant R441H fusion enzyme Homo sapiens
1.14.16.4 0.0217
-
 tetrahydrobiopterin pH 7.0, 30°C, recombinant wild-type TPH2 fusion enzyme Homo sapiens
1.14.16.4 0.0249
-
 tetrahydrobiopterin pH 7.0, 30°C, recombinant mutant R441H fusion enzyme Homo sapiens
1.14.16.4 0.0255
-
 L-tryptophan pH 7.0, 30°C, recombinant mutant P449R fusion enzyme Homo sapiens
1.14.16.4 0.0282
-
 L-tryptophan pH 7.0, 30°C, recombinant wild-type TPH2 fusion enzyme Homo sapiens
1.14.16.4 0.0289
-
 tetrahydrobiopterin pH 7.0, 30°C, recombinant detagged mutant R441H Homo sapiens
1.14.16.4 0.0334
-
 L-tryptophan pH 7.0, 30°C, recombinant detagged mutant R441H Homo sapiens
1.14.16.4 0.0413
-
 L-tryptophan
-
 Homo sapiens
1.14.16.4 0.0413
-
 L-tryptophan pH 7.0, 30°C, recombinant detagged wild-type TPH2 Homo sapiens
1.14.16.4 0.0511
-
 L-tryptophan pH 7.0, 30°C, recombinant detagged mutant P449R Homo sapiens
1.14.16.4 0.0518
-
 tetrahydrobiopterin pH 7.0, 30°C, recombinant detagged mutant P449R Homo sapiens
1.14.16.4 0.0684
-
 (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin
-
 Homo sapiens
1.14.16.4 0.0684
-
 tetrahydrobiopterin pH 7.0, 30°C, recombinant detagged wild-type TPH2 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.16.4 240300
-
 gel-filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.16.4 L-tryptophan + tetrahydrobiopterin + O2 Homo sapiens rate-limiting step in the biosynthesis of serotonin, regulation of isozymes TPH1 and TPH2, overview 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.4 Homo sapiens
-
-
-
1.14.16.4 Homo sapiens Q8IWU9 genes tph1 and tph2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.16.4 recombinant His6-MBP-fusion TPH2 wild-type and mutants from Escherichia coli by affinity chromatography, fusion protein cleavage, and gel filtration Homo sapiens
1.14.16.4 two chromatography steps Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.16.4 cell culture
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.16.4 0.000064
-
 recombinant mutant R441H TPH2 in HEK-293 cells Homo sapiens
1.14.16.4 0.00052
-
 recombinant wild-type TPH2 in HEK-293 cells Homo sapiens
1.14.16.4 0.00165
-
 recombinant mutant P449R TPH2 in HEK-293 cells Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.4 L-tryptophan + (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + O2
-
 Homo sapiens 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin
-
 ?
1.14.16.4 L-tryptophan + tetrahydrobiopterin + O2
-
 Homo sapiens 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin
-
 ?
1.14.16.4 L-tryptophan + tetrahydrobiopterin + O2 rate-limiting step in the biosynthesis of serotonin, regulation of isozymes TPH1 and TPH2, overview Homo sapiens 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.16.4 tetramer structure molecular modelling of TPH2 wild-type and mutants Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.14.16.4 TPH2
-
 Homo sapiens
1.14.16.4 tryptophan hydroxylase
-
 Homo sapiens
1.14.16.4 tryptophan hydroxylase 2
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.16.4 30
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.16.4 7
-
 assay at Homo sapiens