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Literature summary extracted from

  • Martinez-Rodriguez, S.; Clemente-Jimenez, J.M.; Rodriguez-Vico, F.; Las Heras-Vazquez, F.J.
    Molecular cloning and biochemical characterization of L-N-carbamoylase from Sinorhizobium meliloti CECT4114 (2005), J. Mol. Microbiol. Biotechnol., 9, 16-25.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.87 Co2+ 2 mM, specific activity 2.27, relative activity 339% Sinorhizobium meliloti
3.5.1.87 Fe2+ 2 mM, specific activity 2.03, relative activity 303% Sinorhizobium meliloti
3.5.1.87 Mn2+ 2 mM, specific activity 3.24, relative activity 484% Sinorhizobium meliloti
3.5.1.87 Ni2+ 2 mM, specific activity 9.79, relative activity 1461% Sinorhizobium meliloti

Application

EC Number Application Comment Organism
3.5.1.87 biotechnology production of optically pure L-amino acids by an enzymatic method named hydantoinase process Sinorhizobium meliloti

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.87 the L-N-carbamoylase gene is amplified by PCR and cloned into the pBSK vector for expression of the protein in Escherichia coli BL21(DE3) cells Sinorhizobium meliloti

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.87 5,5'-dithiobis-(2-nitrobenzoic acid) specific activity 0.39, relative activity 58% Sinorhizobium meliloti
3.5.1.87 beta-mercaptoethanol specific activity 0.44, relative activity 66% Sinorhizobium meliloti
3.5.1.87 Cu2+ 2 mM, specific activity 0.2, relative activity 30% Sinorhizobium meliloti
3.5.1.87 EDTA 10 mM, specific activity 0, relative activity 0% Sinorhizobium meliloti
3.5.1.87 Fe3+ 2 mM, specific activity 0.44, relative activity 66% Sinorhizobium meliloti
3.5.1.87 Hg2+ 2 mM, specific activity 0, relative activity 0% Sinorhizobium meliloti
3.5.1.87 K+ 2 mM, specific activity 0.56, relative activity 84% Sinorhizobium meliloti
3.5.1.87 Na+ 2 mM, specific activity 0.57, relative activity 85% Sinorhizobium meliloti
3.5.1.87 Pb2+ 2 mM, specific activity 0.39, relative activity 58% Sinorhizobium meliloti
3.5.1.87 Zn2+ 2 mM, specific activity 0.29, relative activity 43% Sinorhizobium meliloti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.87 0.65
-
 N-carbamoyl-L-tryptophan determined at 40°C for 20 min at pH 8.0 after preincubation with 2 mM Ni2+ Sinorhizobium meliloti
3.5.1.87 0.69
-
 N-carbamoyl-L-methionine determined at 40°C for 20 min at pH 8.0 after preincubation with 2 mM Ni2+ Sinorhizobium meliloti
3.5.1.87 0.94
-
 N-carbamoyl-L-alanine determined at 40°C for 20 min at pH 8.0 after preincubation with 2 mM Ni2+ Sinorhizobium meliloti
3.5.1.87 2.61
-
 N-carbamoyl-L-phenylalanine determined at 40°C for 20 min at pH 8.0 after preincubation with 2 mM Ni2+ Sinorhizobium meliloti
3.5.1.87 4.8
-
 N-carbamoyl-L-tyrosine determined at 40°C for 20 min at pH 8.0 after preincubation with 2 mM Ni2+ Sinorhizobium meliloti
3.5.1.87 4.91
-
 N-carbamoyl-L-cysteine determined at 40°C for 20 min at pH 8.0 after preincubation with 2 mM Ni2+ Sinorhizobium meliloti
3.5.1.87 5.5
-
 N-acetyl-L-methionine determined at 40°C for 20 min at pH 8.0 after preincubation with 2 mM Ni2+ Sinorhizobium meliloti
3.5.1.87 12.9
-
 N-formyl-L-methionine determined at 40°C for 20 min at pH 8.0 after preincubation with 2 mM Ni2+ Sinorhizobium meliloti
3.5.1.87 34.47
-
 N-carbamoyl-L-valine determined at 40°C for 20 min at pH 8.0 after preincubation with 2 mM Ni2+ Sinorhizobium meliloti
3.5.1.87 51.23
-
 N-carbamoyl-L-glutamic acid determined at 40°C for 20 min at pH 8.0 after preincubation with 2 mM Ni2+ Sinorhizobium meliloti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.87 42000
-
 SDS-PAGE Sinorhizobium meliloti
3.5.1.87 44860
-
 molecular mass deduced from the amino acid sequence Sinorhizobium meliloti
3.5.1.87 90000
-
 gel filtration Sinorhizobium meliloti

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.87 Sinorhizobium meliloti
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.87 a one-step purification procedure of the recombinant L-N-carbamoylase fused to the HIS6-tag is employed using immobilized cobalt affinity chromatography Sinorhizobium meliloti

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.87 0.66
-
 N-carbamoyl-L-methionine Sinorhizobium meliloti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.87 N-acetyl-L-methionine + H2O
-
 Sinorhizobium meliloti L-methionine + acetate
-
 ?
3.5.1.87 N-carbamoyl-L-alanine + H2O
-
 Sinorhizobium meliloti L-alanine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-cysteine + H2O
-
 Sinorhizobium meliloti L-cysteine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-glutamic acid + H2O
-
 Sinorhizobium meliloti L-glutamic acid + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-methionine + H2O activity assay Sinorhizobium meliloti L-methionine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-phenylalanine + H2O
-
 Sinorhizobium meliloti L-phenylalanine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-tryptophan + H2O
-
 Sinorhizobium meliloti L-tryptophan + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-tyrosine + H2O
-
 Sinorhizobium meliloti L-tyrosine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-valine + H2O
-
 Sinorhizobium meliloti L-valine + CO2 + NH3
-
 ?
3.5.1.87 N-formyl-L-methionine + H2O
-
 Sinorhizobium meliloti L-methionine + HCOOH
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.87 homodimer
-
 Sinorhizobium meliloti

Synonyms

EC Number Synonyms Comment Organism
3.5.1.87 L-N-carbamoylase
-
 Sinorhizobium meliloti
3.5.1.87 N-carbamoyl-L-amino-acid hydrolase
-
 Sinorhizobium meliloti

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.87 60
-
 activity assay Sinorhizobium meliloti

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.87 30 70
-
 Sinorhizobium meliloti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.87 8
-
 activity assay Sinorhizobium meliloti

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.87 6 11
-
 Sinorhizobium meliloti