EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.22 | methanol | 20% v/v, 17% activation | Bos taurus | |
2.4.1.90 | alpha-lactalbumin | alters the substrate specificity of EC 2.4.1.38 to EC 2.4.1.90, and leads to complex formation with the enzyme as lactose synthase, EC 2.4.1.22 | Bos taurus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.1.38 | additional information | covalent immobilization of the enzyme in the absence of alpha-lactalbumin for use as biocatalyst, method evaluation, overview | Bos taurus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.22 | acetone | 20% v/v, 69% inhibition | Bos taurus | |
2.4.1.22 | acetonitrile | 20% v/v, complete inhibition | Bos taurus | |
2.4.1.22 | Dimethylsulfoxide | 20% v/v, 43% inhibition | Bos taurus | |
2.4.1.22 | dioxane | 20% v/v, 88% inhibition | Bos taurus | |
2.4.1.22 | ethanol | 20% v/v, 4% inhibition | Bos taurus | |
2.4.1.22 | N,N-Dimethylformamide | 20% v/v, 90% inhibition | Bos taurus | |
2.4.1.22 | N-methylpyrrolidone | 20% v/v, complete inhibition | Bos taurus | |
2.4.1.22 | tetrahydrofuran | 20% v/v, complete inhibition | Bos taurus | |
2.4.1.38 | alpha-lactalbumin | inhibits the reaction of EC 2.4.1.38 with the complex glucoside colchicoside | Bos taurus | |
2.4.1.90 | acetone | 20% v/v, 37% inhibition | Bos taurus | |
2.4.1.90 | acetonitrile | 20% v/v, complete inhibition | Bos taurus | |
2.4.1.90 | alpha-lactalbumin | - |
Bos taurus | |
2.4.1.90 | Dimethylsulfoxide | 20% v/v, 62% inhibition | Bos taurus | |
2.4.1.90 | ethanol | 20% v/v, 42% inhibition | Bos taurus | |
2.4.1.90 | N,N-Dimethylformamide | 20% v/v, 84% inhibition | Bos taurus | |
2.4.1.90 | N-methylpyrrolidone | 20% v/v, 9% inhibition | Bos taurus | |
2.4.1.90 | tetrahydrofuran | 20% v/v, complete inhibition | Bos taurus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.1.38 | extracellular | - |
Bos taurus | - |
- |
2.4.1.90 | extracellular | - |
Bos taurus | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.38 | Mn2+ | - |
Bos taurus | |
2.4.1.90 | Mn2+ | - |
Bos taurus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.38 | UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide | Bos taurus | - |
UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide | - |
? | |
2.4.1.90 | UDP-galactose + N-acetylglucosamine | Bos taurus | - |
UDP + N-acetyllactosamine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.22 | Bos taurus | - |
- |
- |
2.4.1.38 | Bos taurus | - |
- |
- |
2.4.1.90 | Bos taurus | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.1.22 | milk | - |
Bos taurus | - |
2.4.1.38 | milk | - |
Bos taurus | - |
2.4.1.90 | milk | - |
Bos taurus | - |
EC Number | Storage Stability | Organism |
---|---|---|
2.4.1.38 | 4°C, immobilized, 5 weeks, stable | Bos taurus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.22 | UDP-D-galactose + D-glucose | lactose synthesis is achieved only in the presence of alpha-lactalbumin. In absence of alpha-lactalbumin the enzyme catalyzes the reaction of EC 2.4.1.90 | Bos taurus | UDP + lactose | - |
? | |
2.4.1.38 | additional information | substrate specificity in presence or absence of alpha-lactalbumin, overview, the 'specifier' protein alpha-lactalbumin, which interacts with beta-1,4-GalT forming the lactose synthase complex, EC 2.4.1.2 and changing the substrate specificity to the reaction of EC 2.4.1.90, alpha-lactalbumin is not necessary when the acceptors are different glucopyranosides and, in some cases, it can even have an inhibitory effect, e.g. in reaction with the complex glucosides ginsenoside Rg1 and colchicoside, overview, in absence of alpha-lactalbumin, the enzyme is not active with glucose as acceptor | Bos taurus | ? | - |
? | |
2.4.1.38 | UDP-galactose + colchicoside | - |
Bos taurus | UDP + beta-D-galactosyl-1,4-colchicoside | - |
? | |
2.4.1.38 | UDP-galactose + ginsenoside Rg1 | - |
Bos taurus | UDP + beta-D-galactosyl-1,4-ginsenoside Rg1 | - |
? | |
2.4.1.38 | UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide | - |
Bos taurus | UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide | - |
? | |
2.4.1.38 | UDP-galactose + N-acetylglucosamine | - |
Bos taurus | UDP + N-acetyllactosamine | - |
? | |
2.4.1.90 | additional information | substrate specificity in presence or absence of alpha-lactalbumin, overview, the 'specifier' protein alpha-lactalbumin, which interacts with beta-1,4-GalT forming the lactose synthase complex, EC 2.4.1.22, is not necessary when the acceptors are different glucopyranosides, EC 2.4.1.38, and, in some cases, it can even have an inhibitory effect, e.g. in reaction with the complex glucosides ginsenoside Rg1 and colchicoside, overview | Bos taurus | ? | - |
? | |
2.4.1.90 | UDP-galactose + colchicoside | - |
Bos taurus | ? | - |
? | |
2.4.1.90 | UDP-galactose + D-glucose | reaction of EC 2.4.1.22 | Bos taurus | UDP + lactose | - |
? | |
2.4.1.90 | UDP-galactose + ginsenoside Rg1 | - |
Bos taurus | ? | - |
? | |
2.4.1.90 | UDP-galactose + N-acetyl-D-glucosamine | in presence of alpha-lactalbumin, the enzyme catalyzes reaction of EC 2.4.1.22 | Bos taurus | UDP + N-acetyllactosamine | - |
? | |
2.4.1.90 | UDP-galactose + N-acetylglucosamine | - |
Bos taurus | UDP + N-acetyllactosamine | - |
? | |
2.4.1.90 | UDP-galactose + N-acetylglucosamine | partial reaction of EC 2.4.1.22 | Bos taurus | UDP + N-acetyllactosamine | - |
? | |
2.4.1.90 | UDP-glucose + colchicoside | - |
Bos taurus | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.38 | beta-1,4-galactosyltransferase | - |
Bos taurus |
2.4.1.38 | beta-1,4-GalT | - |
Bos taurus |
2.4.1.38 | More | cf. EC 2.4.1.90 | Bos taurus |
2.4.1.90 | beta-1,4-galactosyltransferase | - |
Bos taurus |
2.4.1.90 | beta-1,4-GalT | - |
Bos taurus |
2.4.1.90 | More | cf. EC 2.4.1.38 and 2.4.1.22 | Bos taurus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.38 | 30 | 37 | assay at | Bos taurus |
2.4.1.90 | 30 | - |
assay at | Bos taurus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.38 | 7.4 | - |
assay at | Bos taurus |
2.4.1.90 | 8.4 | - |
assay at | Bos taurus |