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Literature summary extracted from

  • Athanasopoulos, V.I.; Niranjan, K.; Rastall, R.A.
    Regioselective synthesis of mannobiose and mannotriose by reverse hydrolysis using a novel 1,6-alpha-D-mannosidase from Aspergillus phoenicis (2004), J. Mol. Catal. B, 27, 215-219.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.163 synthesis regioselective synthesis of mannobiose and mannotriose by reverse hydrolysis using the 1,6-alpha-D-mannosidase from Aspergillus phoenicis, method optimization, overview Aspergillus phoenicis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.163 Aspergillus phoenicis
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 ATCC 14332, syn. Aspergillus saitoi
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.163 alpha-D-Manp-(1,6)-D-Manp + H2O the enzyme cleaves specifically (1-6)-linked mannobiose and has no activity towards alpha-D-Manp-1,2-D-Manp or alpha-D-Manp-1, 3-D-Manp Aspergillus phoenicis alpha-D-mannose
-
 ?
3.2.1.163 additional information regioselective synthesis of mannobiose and mannotriose by reverse hydrolysis of commercially available manno-oligosaccharide Aspergillus phoenicis ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.163 55
-
-
 Aspergillus phoenicis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.163 45 70 21% of maximal activity at 45°C, 16% at 70°C Aspergillus phoenicis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.163 4.5
-
-
 Aspergillus phoenicis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.163 3.5 6
-
 Aspergillus phoenicis